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Gene Review

tRNA-Met  -  tRNA

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Disease relevance of tRNA-Met


High impact information on tRNA-Met


Biological context of tRNA-Met

  • The absence of summation of the rate of methylation of positionally analogous cytidine residues in tRNA1Val, tRNAPhe, and tRNAMet in the case of simultaneous presence of two substrates in the incubation mixture was demonstrated by the method of mixed substrates [6].

Associations of tRNA-Met with chemical compounds

  • The NMR spectra of the two tRNA species in the region between 0 and 4 ppm below 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) (methyl and methylene region) were the same except for the absence of the lowest field peak at 3.8 ppm in tRNAMet f3, thus unequivocally identifying this resonance at the methyl group of m7G47 of tRNAMet f1 [1].
  • Of the two species of tRNAMet, one can be formylated in the presence of formyl tetrahydrofolate and the transformylase from mycobacteria [7].

Other interactions of tRNA-Met


Analytical, diagnostic and therapeutic context of tRNA-Met

  • To confirm this notion more clearly, the tertiary structure of Ascaris suum mt tRNAMet was analyzed by NMR using various synthetic tRNAs site-specifically labeled with stable isotopes, which were prepared by a combination of chemical synthesis and enzymatic ligation [9].


  1. Changes in tertiary structure accompanying a single base change in transfer RNA. Proton magnetic resonance and aminoacylation studies of Escherichia coli tRNAMet f1 and tRNAMet f3 and their spin-labeled (s4U8) derivatives. Daniel, W.E., Cohn, M. Biochemistry (1976) [Pubmed]
  2. The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA. Anderson, J., Phan, L., Cuesta, R., Carlson, B.A., Pak, M., Asano, K., Björk, G.R., Tamame, M., Hinnebusch, A.G. Genes Dev. (1998) [Pubmed]
  3. Wheat germ splicing endonuclease is highly specific for plant pre-tRNAs. Stange, N., Gross, H.J., Beier, H. EMBO J. (1988) [Pubmed]
  4. Mammalian mitochondrial methionyl-tRNA transformylase from bovine liver. Purification, characterization, and gene structure. Takeuchi, N., Kawakami, M., Omori, A., Ueda, T., Spremulli, L.L., Watanabe, K. J. Biol. Chem. (1998) [Pubmed]
  5. A single base change in the intron of a serine tRNA affects the rate of RNase P cleavage in vitro and suppressor activity in vivo in Saccharomyces cerevisiae. Willis, I., Frendewey, D., Nichols, M., Hottinger-Werlen, A., Schaack, J., Söll, D. J. Biol. Chem. (1986) [Pubmed]
  6. Use of the method of mixed substrates to study the specificity of tRNA methylases. Gambaryan, A.S., Venkstern, T.V., Baev, A.A. Mol. Biol. (Mosk.) (1976) [Pubmed]
  7. Studies on transfer RNA from mycobacteria. Deobagkar, D.N., Gopinathan, K.P. Can. J. Microbiol. (1978) [Pubmed]
  8. Catalytic activation of transfer ribonucleic acid by a mammalian protein. Dickman, S.R., Boll, D.J. Biochemistry (1976) [Pubmed]
  9. Stable isotope-edited NMR analysis of Ascaris suum mitochondrial tRNAMet having a TV-replacement loop. Ohtsuki, T., Kawai, G., Watanabe, K. J. Biochem. (1998) [Pubmed]
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