Disease relevance of H2-Eb1

• Using the spheroplast fusion technique, we have introduced the cloned E beta b gene into two d haplotype cell lines, the B lymphoma line A20-2J and the macrophage tumor line P388D1 [1].

High impact information on H2-Eb1

• To define the extent of sequence shared between the I-E beta product and the mutant I-A beta product, we isolated a cDNA clone of the E beta b gene and determined its nucleotide sequence [2].
• A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes [3].
• The nucleotide sequence of the murine I-E beta b immune response gene: evidence for gene conversion events in class II genes of the major histocompatibility complex [4].
• In combination with directed mutagenesis, the system described here provides a means to study (i) E beta b sequences that are important in determining the restriction specificity of the E molecule and (ii) sequences associated with the E beta gene that may be important in the regulation of E beta chain expression [1].
• Reciprocally, the replacement of glutamic acid at beta 29 in E beta b with valine results in a gain of pigeon peptide binding [5].

Biological context of H2-Eb1

• We have determined the DNA sequence of the murine I-E beta b immune response gene of the major histocompatibility complex (MHC) of the C57BL/10 mouse and compared it with the sequence of allelic I-E and non-allelic I-A genes from the d and k haplotypes [4].
• Here we demonstrate that already the expression of single MHC class II chains (E alpha k and E beta b) is sufficient to cause diabetes without affecting beta cell morphology and cell numbers [6].
• The H2 Eb alleles were discerned by restriction fragment length polymorphism (RFLP) and heteroduplex analyses [7].

Anatomical context of H2-Eb1

• Although the transgenic E alpha k E beta b MHC class II molecules were expressed on the surface of pancreatic beta cells, the transgenic mice did not prove to be tolerant for I-E antigen [6].
• Interaction of H-2Eb with an IAP retrotransposon in the A20/2J B cell lymphoma [8].

Associations of H2-Eb1 with chemical compounds

• Sequence analysis of two independently isolated E beta k cDNA clones revealed that the E beta k molecule is identical to the E beta b molecule at the NH2-terminus [9].
• The recombinant H-2 haplotype was designated bq5 and possesses the following alleles at individual loci of the H-2 complex: Kb A beta b A alpha b E beta b or q E alpha b or q Sb or q Dq Lq [10].
• The replacement of the valine found at position beta 29 in E beta k with the glutamic acid found in E beta b results in a selective loss of pigeon cytochrome peptide but not moth cytochrome peptide binding to the resultant mutant E alpha E beta k molecule [5].

Other interactions of H2-Eb1

• We show here that the E beta b gene is identical to the non-allelic A beta bm12 DNA sequence in the mutated region and suggest, therefore, that the E beta b gene was the donor sequence for this intergenic transfer of genetic information [4].

Analytical, diagnostic and therapeutic context of H2-Eb1

• While expression of E alpha dE beta b is constitutive in A20-2J cells transfected with the E beta b gene, surface expression of E alpha dE beta b is detected in transfected macrophage cells only after treatment of cells with culture supernatants from concanavalin A (Con A)-stimulated T cells [1].

References