High impact information on Prei3

• Phocein, an intracellular protein interacting with striatin, bears a few homologies with the varsigma-subunits of clathrin adaptor proteins (Baillat, G., Moqrich, A., Castets, F., Baude, A., Bailly, Y., Benmerah, A., and Monneron, A. (2001) Mol. Biol. Cell 12, 663-673) [1].
• Molecular cloning and characterization of phocein, a protein found from the Golgi complex to dendritic spines [2].
• Fractionation of brain and HeLa cells showed that phocein is associated with membranes, as well as present in the cytosol where it behaves as a protein complex [2].
• The postsynaptic profiles containing phocein were engaged in asymmetric synapses with the main types of afferents in the cerebellum and in the hippocampus [3].
• Immunogold labeling was performed to assess subcellular localization of phocein in neurons of the rodent cerebellar cortex and hippocampal Ammon's horn [3].

Anatomical context of Prei3

• Expression and distribution of phocein and members of the striatin family in neurones of rat peripheral ganglia [4].
• Our study thus demonstrates that phocein and the members of the striatin family are expressed not only in central nervous system but also in the peripheral nervous system and, in particular, in afferent sensory neurones [4].
• The distribution appears to differ: immunoreactivity for striatin and SG2NA is found only in soma of sensory neurons, whereas immunoreactivity for phocein is observed in both soma and processes [4].
• Immunocytochemical detections demonstrate that phocein, striatin and SG2NA are expressed in neurones of vagal, petrosal and dorsal root ganglia [4].
• Immunoblotting experiments confirm these data and in addition demonstrate that: (1) the proteins phocein, striatin and SG2NA are also present in the superior cervical ganglion and (2) zinedin is detected in all studied ganglia [4].

Associations of Prei3 with chemical compounds

• Phocein and members of the striatin family (striatin, SG2NA and zinedin) are intracellular proteins, mainly expressed in neurones of the mammalian central nervous system where they are thought to be involved in vesicular traffic and Ca(2+) signalling [4].

Analytical, diagnostic and therapeutic context of Prei3

• RT-PCR experiments showed that mRNAs of phocein, striatin, SG2NA and zinedin are present in all studied peripheral ganglia [4].

References