High impact information on Slc4a2

• Among the four AE genes identified in mammals thus far, only Ae2 (Slc4a2) has been determined to be expressed in the male reproductive system, especially in developing spermatozoa and in epididymal epithelium [1].
• Here, we generated mice carrying a targeted disruption of Ae2 that prevents the expression of the three AE2 isoforms (Ae2a, Ae2b1, and Ae2b2) normally found in mouse testes [1].
• Our findings reveal an essential role of Ae2 in mouse spermiogenesis and stress the recently postulated involvement of bicarbonate in germ-cell differentiation through the bicarbonate-sensitive soluble-adenylyl-cyclase pathway [1].
• In parietal cells, basolateral Ae2 Cl(-)/HCO(3)(-) exchanger (Slc4a2) appears to compensate for luminal H(+) pumping while providing Cl(-) for apical secretion [2].
• In the oxyntic mucosa of Ae2(a,b)(-/-) mice, total Ae2 protein was nearly undetectable, indicating low gastric expression of the Ae2c isoforms [2].

Biological context of Slc4a2

• The carboxyl-terminal hydrophobic domain of B3RP has an amino acid sequence 67% identical to that of band 3, with a very similar predicted secondary structure [3].

Anatomical context of Slc4a2

• In this study, an epitope-unmasking technique was used to immunolocalize AE2 anion exchanger polypeptide to basolateral plasma membranes of tubular epithelial cells in mouse kidney [4].
• Proteolytic cleavage sites of native AE2 anion exchanger in gastric mucosal membranes [5].
• NH4Cl activates AE2 anion exchanger in Xenopus oocytes at acidic pHi [6].
• Zebrafish Ae2 also mediates Cl(-)/HCO(3)(-) exchange in X. laevis oocytes and accumulates in or near the plasma membrane in transfected HEK-293 cells [7].

Associations of Slc4a2 with chemical compounds

• In the course of experiments to define regulation by intracellular pH (pHi) of the AE2 anion exchanger expressed in Xenopus oocytes, we discovered an unexpected regulation of AE2 by NH4+ [6].

Other interactions of Slc4a2

• The other, far amino-terminal section of B3RP has no correspondent in the band 3 sequence [3].

Analytical, diagnostic and therapeutic context of Slc4a2

• Molecular cloning and genomic organization of the mouse AE2 anion exchanger gene [8].

References