High impact information on CLEC5A

• Kinetics of the ATP hydrolysis cycle of the nucleotide-binding domain of Mdl1 studied by a novel site-specific labeling technique [1].
• In contrast, mutating the other DCL, MDL-1, exhibited a gene silencing frequency similar to wild type and accumulated siRNA normally [2].
• These results indicate that only MDL-2 is responsible for siRNA production, and no functional redundancy exists between MDL-1 and MDL-2 in the RNA silencing pathway in M. oryzae [2].
• Two DCL genes, MDL-1 and -2 (Magnaporthe Dicer-like-1 and -2) have been identified in the genome of the filamentous fungus Magnaporthe oryzae [2].
• Like MDL1 and MDL3 cDNAs (Z. Hu, J.E. Poulton [1997] Plant Physiol 115: 1359-1369), they had open reading frames that predicted a flavin adenine dinucleotide-binding site, multiple N-glycosylation sites, and an N-terminal signal sequence [3].

Anatomical context of CLEC5A

• TREM-1 and MDL-1 RNA expression was also more elevated in adult than fetal tissues and in normal than malignant cells [4].
• Northern and RT-PCR revealed a strong expression of TREM-1, MDL-1, and DAP12 in peripheral blood-derived CD14(+) mature monocytes in contrast to undifferentiated bone marrow CD34(+) stem cells, and in the differentiated versus undifferentiated U937 cells [4].

Other interactions of CLEC5A

• TREM-1, MDL-1, and DAP12 expression is associated with a mature stage of myeloid development [4].

Analytical, diagnostic and therapeutic context of CLEC5A

• Northern-blot analysis using gene-specific probes revealed differential expression of the genes mdl1, mdl2, mdl3, mdl4, and mdl5 [3].
• High energy pulsed dye laser lithotripsy (Candela MDL-2000), with energy output upgraded to a maximum of 140 mJ. at the laser fiber tip using the 320 mu core fiber, was compared to the initially commercialized device, with the energy output fixed at 60 mJ. using the 200 mu core fiber (Candela MDL-1) [5].

References