Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

Glycam1 - glycosylation dependent cell adhesion...

Rattus norvegicus

Synonyms: Endothelial ligand FOR L-selectin, GlyCAM-1, Glycosylation-dependent cell adhesion molecule 1, SGP50, Sulfated 50 kDa glycoprotein

Dowbenko, D. et al., Kanoh, N. et al., Derry, C.J. et al.

Kanoh, Dai, Tanaka, Izawa, Li, Kawashima, Miyasaka, Derry, Faveeuw, Mordsley, Ager,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on Glycam1

This ligand has been named GlyCAM 1 (Gly-cosylation-dependent Cell Adhesion Molecule 1) because its adhesive interactions with the L selectin lectin domain require that the GlyCAM 1 polypeptide chain be appropriately modified with carbohydrates [1].
An additional interesting structure that may have relevance to the association of GlyCAM 1 with the lumenal surface of the endothelium was a potential amphipathic helix at the C terminus of the glycoprotein [1].
Finally, immunoprecipitation analysis of [35S]sulfate-labeled rat lymph nodes with either a mouse L selectin IgG chimera or a peptide antiserum directed against a relatively conserved portion of mouse GlyCAM 1 demonstrates a approximately 45-kDa sulfated ligand in rat lymph nodes that is analogous to that previously described for mouse lymph nodes [1].
The sequence of this clone reveals a serine/threonine-rich protein that is highly homologous with the mouse GlyCAM 1 [1].
HEV express several L-selectin ligands including CD34, GlyCAM-1, MAdCAM-1 and two sulfated glycoproteins (Sgp) of 200 kDa and 170 kDa which remain to be identified [2].

Biological context of Glycam1

These observations suggest that GlyCAM-1 expressed in the cochlear region is heterogenous in terms of its glycosylation [3].

Anatomical context of Glycam1

Here we report that GlyCAM-1 is also expressed in the cochlea [3].
Electron microscopic investigation revealed the presence of GlyCAM-1 over the entire lumenal surface of the vessels, and the basolateral infoldings in stria vascularis [3].
Glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1) is a mucin-like glycoprotein previously identified on high endothelial venules (HEV) of lymph nodes and also in lactating mammary glands [3].

Analytical, diagnostic and therapeutic context of Glycam1

Moreover, RT-PCR of the cochlear tissue by the use of specific oligonucleotide primers for rat GlyCAM-1 generated a 378 bp product which was then verified by nucleotide sequencing to represent GlyCAM-1 [3].

References

Cloning of a rat homologue of mouse GlyCAM 1 reveals conservation of structural domains. Dowbenko, D., Watson, S.R., Lasky, L.A. J. Biol. Chem. (1993) [Pubmed]
Novel chondroitin sulfate-modified ligands for L-selectin on lymph node high endothelial venules. Derry, C.J., Faveeuw, C., Mordsley, K.R., Ager, A. Eur. J. Immunol. (1999) [Pubmed]
Constitutive expression of GlyCAM-1 core protein in the rat cochlea. Kanoh, N., Dai, C.F., Tanaka, T., Izawa, D., Li, Y.F., Kawashima, H., Miyasaka, M. Cell Adhes. Commun. (1999) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.