High impact information on IDH1

• In addition, we found that the trans-10, cis-12 CLA, but not the cis-9, trans-11 CLA isomer, inhibited basal- and PRL-induced IDH1 mRNA expression [1].
• We propose that the inhibitory effects of CLA on milk fat synthesis in mammary epithelial cells may derive, at least in part, from repression of IDH1 expression [1].
• In previous studies, we documented that the expression of IDH1 in bovine mammary epithelium was modulated by regulators of mammary differentiation and metabolic effectors [1].
• The cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDH1) plays a critical role in cholesterol and fatty acid biosynthesis by providing reducing equivalents as NADPH [1].
• Next, we examined the effects of extracellular matrix and lactogenic hormones on the expression of IDH1 in cultured BME-UV bovine mammary epithelial cells [2].

Biological context of IDH1

• The enzymatic activity of IDH1 increases dramatically in early lactation in bovine mammary tissue [2].
• IDH1 mRNA levels increased by 2.3-fold after parturition compared to late pregnancy and remained elevated thereafter [2].

Anatomical context of IDH1

• We found that expression of IDH1 mRNA increased in parallel with beta-casein expression induced by extracellular matrix [2].
• The cytosolic NADP(+)-dependent isocitrate dehydrogenase (IDH1) catalyzes the conversion of isocitrate to alpha-ketoglutarate in the cytosol, and generates NADPH as a primary source of reducing equivalents for de novo fatty acid synthesis in bovine mammary gland [2].

Associations of IDH1 with chemical compounds

• Finally, we report that treatment of BME-UV cells with alpha-ketoglutarate and palmitic acid reduced IDH1 transcript levels [2].

Other interactions of IDH1

• In this study, we investigated the short-term effects of prolactin (PRL) and CLA on IDH1 expression in BME-UV bovine mammary epithelial cells [1].

Analytical, diagnostic and therapeutic context of IDH1

• The inhibitory effects of the trans-10, cis-12 CLA isomer on PRL-induced IDH1 expression accumulation were confirmed by quantitative real time PCR and western-blotting analysis [1].

References