Disease relevance of GUCY2F

• We identified somatic mutations in GUCY2F, EPHA3, and NTRK3 in breast, lung, and pancreatic cancers [1].
• The major form of GCF has a molecular weight of approximately M(r) 97,000, identical to that of GCF transiently expressed in CV1 cells by the vaccinia virus system [2].
• These findings indicate that GCF negatively regulates gene expression of not only the EGFR but also several other growth factor and receptor genes and can inhibit the growth of gastric carcinomas in immunodeficient mice [3].

High impact information on GUCY2F

• By in situ hybridization, mRNA encoding RetGC-2 is found only in the outer nuclear layer and inner segments of photoreceptor cells [4].
• By using synthetic peptide antiserum specific for each RetGC subtype, RetGC-2 can be distinguished from RetGC-1 as a slightly smaller protein in immunoblots of bovine rod outer segments [4].
• Substitution of these three domains in GCAP-2 with corresponding neurocalcin sequences also affects activation of individual recombinant RetGC-1 and RetGC-2 expressed in HEK293 cells [5].
• These results suggested that GC-E and GC-F could form either homomers or heteromers, at least when overexpressed in COS-7 cells [6].
• 1. The human GC-F gene was localized to the long arm of chromosome Xq22 by fluorescence in situ hybridization [7].

Biological context of GUCY2F

• In this study, by the use of GCF transfection, we examined whether GCF represses the gene expression of several other growth factors and receptors and causes growth inhibition of cancer cells [3].
• GCF antibodies recognize GCF present in extracts from human cells and causes a "supershift" of a protein DNA complex containing a GCF oligonucleotide binding site [2].
• The GC factor (GCF) binds to specific GC-rich sequences in the epidermal growth factor receptor (EGFR) gene promoter and represses its transcription [3].
• GC-E and GC-F are colocalized within the same photoreceptor cells of the retina and have an important role in phototransduction [8].

Anatomical context of GUCY2F

• In addition, GCF is a phosphoprotein, and the phosphorylated form is found to be associated with the nuclear compartment in both HUT102 and KB cells [2].
• GCF is highly expressed in EGF receptor-negative human cell lines (HUT102, U266, and CA46) and in lower amounts in several EGF receptor-expressing cells (KB, A431, TMK, and HeLa) [2].

Associations of GUCY2F with chemical compounds

• Both cyclases named ROS-GC1 and ROS-GC2 form a subclass of membrane bound cyclases and differ in many aspects from hormone peptide receptor guanylate cyclases [9].

Other interactions of GUCY2F

• Somatic mutations of GUCY2F, EPHA3, and NTRK3 in human cancers [1].

Analytical, diagnostic and therapeutic context of GUCY2F

• Immunoprecipitation with GC-E and GC-F antibody followed by Western analysis confirmed that both homomers and heteromers could be formed [6].
• Cell fractionation studies indicate that GCF is predominantly localized in the nucleus [2].

References