High impact information on TAS2R65P

• There was a high level of PS4 mRNA in rabbit fetal muscle, esophagus, kidney, and lung, a low level in fetal aorta and heart, and an undetectable level in fetal liver, brain, as well as, in the placenta [1].
• The induction of PS4 by serum was completely blocked by cycloheximide, indicating its expression required prior protein synthesis [1].
• Serum stimulation of quiescent smooth muscle cells in culture induced a rapid increase in the level of PS4 mRNA [1].
• We have used subtraction cloning to isolate a cDNA (PS4) that identified a serum-inducible mRNA of 1.9 kilobases in rabbit vascular smooth muscle cells [1].
• Our analysis indicate that PS4 expression is developmentally regulated and tightly controlled by growth factors, suggesting this novel gene has a role in cell growth and differentiation [1].

Biological context of TAS2R65P

• A serological response to PS3 and PS4 was found in 29% and 49% of the patients, respectively, and was correlated with CD4 + cell count [2].

Anatomical context of TAS2R65P

• The PS4 levels in hippocampus and amygdala were significantly correlated with the immobility time in the Porsolt forced swim test, an established animal model for antidepressant effects [3].
• We found that electroconvulsive seizures significantly elevated the PS4 levels in hippocampus, amygdala, pyriform (olfactory) cortex, and anterior cortex but not in striatum, motor cortex, locus ceruleus, or ventral lateral medulla [3].
• The PS4 levels in peripheral blood, hypothalamus, anterior cortex, amygdala, and eyes increased severalfold at 20 min following intracisternal injection of 228 microg of this peptide, suggesting that it readily crosses the blood-brain barrier [3].
• In order to gain insight into the relatively late appearance in ontogeny of responsiveness to T-cell independent (TI) antigens, the in vitro human B-cell response to type 4 pneumococcal polysaccharides (PS4, a human TI-2 antigen) was studied [4].
• B cells obtained from adults differentiate into anti-PS4 antibody forming cells upon culturing with PS4, but neonatal B cells obtained from cord blood fail to respond [4].

Associations of TAS2R65P with chemical compounds

• The screw helices are built up from uranium atoms interconnected by PS4 tetrahedral units [5].
• Fresh rat and human serum rapidly degraded PS4, indicating that it may act primarily as a paracrine modulator of TRH effects in pituitary, brain, and reproductive system [3].
• Serum samples were collected before vaccination and at weeks 1, 2, 4, and 12 after vaccination and were tested for the presence of antibodies against a mixture of capsular polysaccharide antigens (pool) and against type 3 and type 4 antigens (PS3 and PS4), using an ELISA [2].

Analytical, diagnostic and therapeutic context of TAS2R65P

• Sera from 20% of S. mansoni-infected HIV-seronegative individuals tested recognized the PS4 peptide in an ELISA as did sera from S. mansoni-infected rats [6].
• We report the development of a radioimmunoassay for prepro-TRH(160-169) (PS4), a thyrotropin-releasing hormone (TRH) enhancing peptide, and its use in characterizing the effect of electroconvulsive seizures on the levels of this peptide in various brain regions of male Wistar rats [3].

References