High impact information on emb

• Antagonistic regulation of Yan nuclear export by Mae and Crm1 may increase the stringency of the Ras response [1].
• The activity of this NES is inhibited by the antibiotic Leptomycin B, suggesting that Exd is exported by a CRM1/exportin1-related export pathway [2].
• We propose that a major function of DNup88 is to anchor DNup214 and CRM1 on the nuclear envelope and thereby attenuate NES-mediated nuclear export [3].
• Overexpression of DNup88 is sufficient to relocalize DNup214 and CRM1 on the nuclear envelope and revert the mutant phenotypes [3].
• We show that the nuclear accumulation of the Drosophila Rel protein Dorsal requires CRM1 [3].

Biological context of emb

• Nuclear import of an nuclear localization signal-enhanced green fluorescent protein (NLS-EGFP) reporter is not affected in DNup88 (members only; mbo) mutants, whereas the level of CRM1-dependent EGFP-nuclear export signal (EGFP-NES) export is increased [3].
• Through mutagenesis screens we have recovered a series of recessive lethal emb mutations [4].

Anatomical context of emb

• The emb 2.7-kb transcript was expressed in mast cells, myeloid cells and B lymphocytes while the emt 4.6-kb mRNA in mast cells, myeloid cells and T lymphocytes [5].

Associations of emb with chemical compounds

• A leptomycin B-sensitive homologue of human CRM1 promotes nuclear export of nuclear export sequence-containing proteins in Drosophila cells [6].
• The CRM1 (Exportin 1) protein is a receptor for leucine-rich nuclear export signal sequences [4].

Physical interactions of emb

• Nup214 bound to CRM1 directly and anchored it to the nuclear envelope [7].

Other interactions of emb

• The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export [3].
• We demonstrate that the molecular mechanism underlying downregulation of YAN involves CRM1-mediated nuclear export and define a novel role in this context for MAE, a co-factor previously implicated in facilitating MAPK phosphorylation of YAN [8].
• The Drosophila embargoed gene is required for larval progression and encodes the functional homolog of schizosaccharomyces Crm1 [4].

References