High impact information on gammaTub37C

• The murine PL10 cDNA corresponds to a transcript expressed only in the male germ line [1].
• Mitotic phosphorylation of GCP-WD is required for association of gamma-tubulin with the spindle, separately from association with the centrosome [2].
• Drosophila melanogaster gamma-TuRC is dispensable for targeting gamma-tubulin to the centrosome and microtubule nucleation [3].
• In Drosophila melanogaster there are two gamma-tubulin genes, gammaTUB23C and gammaTUB37C [4].
• Microtubule nucleation in vivo requires gamma-tubulin, a highly conserved component of microtubule-organizing centers [4].

Biological context of gammaTub37C

• Cytological characterisation of the mutant phenotypes produced during early embryogenesis by null and loss-of-function alleles of the gammaTub37C gene in Drosophila [5].
• Gamma-tub37CD is constantly detected at the centrosome and absent in the mitotic spindle, while gamma-tub23C is extensively recruited to the centrosome during mitosis and relocalizes in the mitotic spindle [6].
• Differential expression of two gamma-tubulin isoforms during gametogenesis and development in Drosophila [7].
• It is required for centrosome maturation on M-phase entry in order to recruit the gamma-tubulin ring complex and activate the abnormal spindle protein, Asp [8].
• Eggs lacking the maternal gamma-tubulin were arrested in meiosis, indicative of a deficiency in activation [9].

Anatomical context of gammaTub37C

• Unfertilised laid oocytes and fertilised embryos deficient for gammaTub37C do not contain polar bodies and have a few bipolar microtubule arrays [5].
• At stage 10b, we find gammaTub37C and Dgrip75 anteriorly concentrated and propose the formation of a microtubule-organizing center at the anterior pole of the oocyte [10].
• In contrast, gamma-tub37CD is undetectable in the soluble fraction and exhibits a tight binding to the centrosome [6].
• Using specific nucleotidic and immunological probes, we have demonstrated that the two divergent Drosophila gamma-tubulins, gamma-tub23C and gamma-tub37CD, are expressed in cultured cells [6].
• Conversely, gamma Tub37CD was not detected in proliferating germ cells, but appeared to accumulate in germ cells during egg chamber development [7].

Physical interactions of gammaTub37C

• Gamma-tubulin37C and gamma-tubulin ring complex protein 75 are essential for bicoid RNA localization during drosophila oogenesis [10].

Other interactions of gammaTub37C

• These MTOCs appear to be canonical centrosomes because they contain gamma-tubulin, CP190, and centrioles and they undergo duplication [11].

Analytical, diagnostic and therapeutic context of gammaTub37C

• Immunofluorescence analysis of laid eggs and activated oocytes derived from the mutants revealed microtubules and meiotic spindles that were close to normal even in the absence of gamma Tub37CD [9].

References