The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

asp  -  abnormal spindle

Drosophila melanogaster

Synonyms: ASP, Asp, CG6875, Dm Asp, Dmel\CG6875, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of asp

  • Two mutations in this domain, Asp 47----Glu, and Asp 64----Asn, present in patients with haemophilia B, reduce calcium binding to the domain greater than 4-fold and greater than 1,000-fold, respectively [1].

High impact information on asp


Biological context of asp


Anatomical context of asp

  • RNAi depletion of the abnormal spindle protein, Asp, which localizes to focused poles of control spindles, produced a severe loss of spindle pole focus, whereas depletion of the pole-associated microtubule depolymerase KLP10A increased spindle microtubule density [8].
  • A mutant beta-actin with an amino acid substitution from Gly-245 to Asp has been shown to be related to tumorigenic transformation of a human fibroblast cell line (Leavitt, J. et al. (1987) Mol. Cell. Biol. 7, 2467-2476) [9].
  • In contrast, a mutant in which the phosphorylation sites are replaced by phosphate-mimetic Asp residues, as well as a VP16-D-Jun fusion protein, can promote photoreceptor differentiation [10].

Associations of asp with chemical compounds

  • After a 4-h labeling period, we detected completed actin polypeptide chains with either an unblocked Asp or an Ac-Asp NH2 terminus [11].
  • Nevertheless, the most common variants within F and S are distinguished by only two amino acids (Asn/Asp at 237 and Thr/Ala at 247), and these are the most likely targets for the selection underlying complementary latitudinal clines in F and S frequencies [12].
  • Each protein contained a pair of active site motifs (Asp/Thr or Ser/Gly), which is a common characteristic of aspartic proteases including BACE1 [13].

Enzymatic interactions of asp

  • Previous studies with Drosophila actin showed that the first detectable intermediate is one with an Ac-Cys NH2 terminus which is subsequently cleaved in a novel reaction to expose the Asp [14].

Other interactions of asp

  • Centrosomin (CNN) remains in broad discrete bodies but only at the focused poles of such spindles, whereas Asp (abnormal spindle protein) is always present at the presumptive minus ends of microtubules, whether or not they are focused [15].
  • This can be rescued by addition of phosphorylated Asp or active Polo kinase [16].
  • abnormal spindle, a gene required for normal spindle structure and function in Drosophila melanogaster, lies immediately adjacent the gene tolloid at 96A/B [5].
  • However, when this Ser was changed to Asp or Glu, transformation and transcriptional repression by v-Rel were significantly inhibited and c-Rel showed a diffuse nuclear and cytoplasmic localization in CEF [17].
  • These thin central spindles exhibited diffuse localizations of both the Pav and Asp proteins, suggesting that these spindles comprise improperly oriented MTs [18].

Analytical, diagnostic and therapeutic context of asp

  • We have used site-directed mutagenesis to change all of the conserved Ser, His, and Asp residues and have found that Ser120, His157, and Asp135 are all required for activity [19].


  1. Key residues involved in calcium-binding motifs in EGF-like domains. Handford, P.A., Mayhew, M., Baron, M., Winship, P.R., Campbell, I.D., Brownlee, G.G. Nature (1991) [Pubmed]
  2. Nucleotide sequence of the rat skeletal muscle actin gene. Zakut, R., Shani, M., Givol, D., Neuman, S., Yaffe, D., Nudel, U. Nature (1982) [Pubmed]
  3. Abnormal spindle protein, Asp, and the integrity of mitotic centrosomal microtubule organizing centers. do Carmo Avides, M., Glover, D.M. Science (1999) [Pubmed]
  4. A novel motif governs APC-dependent degradation of Drosophila ORC1 in vivo. Araki, M., Yu, H., Asano, M. Genes Dev. (2005) [Pubmed]
  5. The Drosophila gene abnormal spindle encodes a novel microtubule-associated protein that associates with the polar regions of the mitotic spindle. Saunders, R.D., Avides, M.C., Howard, T., Gonzalez, C., Glover, D.M. J. Cell Biol. (1997) [Pubmed]
  6. A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila. Riparbelli, M.G., Callaini, G., Glover, D.M., Avides, M.d.o. .C. J. Cell. Sci. (2002) [Pubmed]
  7. Polo kinase and progression through M phase in Drosophila: a perspective from the spindle poles. Glover, D.M. Oncogene (2005) [Pubmed]
  8. Spindle pole organization in Drosophila S2 cells by dynein, abnormal spindle protein (Asp), and KLP10A. Morales-Mulia, S., Scholey, J.M. Mol. Biol. Cell (2005) [Pubmed]
  9. Actin with tumor-related mutation is antimorphic in Drosophila muscle: two distinct modes of myofibrillar disruption by antimorphic actins. Sakai, Y., Okamoto, H., Mogami, K., Yamada, T., Hotta, Y. J. Biochem. (1990) [Pubmed]
  10. Phosphorylation of Drosophila Jun by the MAP kinase rolled regulates photoreceptor differentiation. Peverali, F.A., Isaksson, A., Papavassiliou, A.G., Plastina, P., Staszewski, L.M., Mlodzik, M., Bohmann, D. EMBO J. (1996) [Pubmed]
  11. NH2-terminal processing of Drosophila melanogaster actin. Sequential removal of two amino acids. Rubenstein, P.A., Martin, D.J. J. Biol. Chem. (1983) [Pubmed]
  12. Amino acid polymorphisms for esterase-6 in Drosophila melanogaster. Cooke, P.H., Oakeshott, J.G. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  13. Characterization of Drosophila aspartic proteases that induce the secretion of a Golgi-resident transferase, heparan sulfate 6-O-sulfotransferase. Kotani, N., Kitazume, S., Kamimura, K., Takeo, S., Aigaki, T., Nakato, H., Hashimoto, Y. J. Biochem. (2005) [Pubmed]
  14. Alternate pathways for removal of the class II actin initiator methionine. Martin, D.J., Rubenstein, P.A. J. Biol. Chem. (1987) [Pubmed]
  15. Mutation of a Drosophila gamma tubulin ring complex subunit encoded by discs degenerate-4 differentially disrupts centrosomal protein localization. Barbosa, V., Yamamoto, R.R., Henderson, D.S., Glover, D.M. Genes Dev. (2000) [Pubmed]
  16. Polo kinase and Asp are needed to promote the mitotic organizing activity of centrosomes. do Carmo Avides, M., Tavares, A., Glover, D.M. Nat. Cell Biol. (2001) [Pubmed]
  17. A protein kinase-A recognition sequence is structurally linked to transformation by p59v-rel and cytoplasmic retention of p68c-rel. Mosialos, G., Hamer, P., Capobianco, A.J., Laursen, R.A., Gilmore, T.D. Mol. Cell. Biol. (1991) [Pubmed]
  18. Feo, the Drosophila homolog of PRC1, is required for central-spindle formation and cytokinesis. Vernì, F., Somma, M.P., Gunsalus, K.C., Bonaccorsi, S., Belloni, G., Goldberg, M.L., Gatti, M. Curr. Biol. (2004) [Pubmed]
  19. Peptidase E, a peptidase specific for N-terminal aspartic dipeptides, is a serine hydrolase. Lassy, R.A., Miller, C.G. J. Bacteriol. (2000) [Pubmed]
WikiGenes - Universities