The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

SPAM1  -  sperm adhesion molecule 1 (PH-20...

Bos taurus

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on SPAM1

  • Bovine testicular hyaluronidase PH-20 activates JNK1 and protects L929 fibroblasts from staurosporine-mediated cell death [1].
  • In AtT-20 cell lines transfected with a complementary DNA (cDNA) encoding the full-length bPAM precursor, both SPAM and PAM activities were increased compared to wild type cells; both activities were decreased in a cell line expressing an antisense rat (r)PAM mRNA [2].
  • Like PAM, SPAM activity is localized to the secretory granule [2].
  • The soluble hyaluronidase from bull testes is a fragment of the membrane-bound PH-20 enzyme [3].
  • Characterization of PH-20 in canine spermatozoa and testis [4].

Biological context of SPAM1


Anatomical context of SPAM1

  • In marked contrast, an AtT-20 cell line transfected with a cDNA encoding a truncated, soluble form of bPAM had elevated levels of PAM activity, but levels of SPAM activity were not increased compared to wild-type cells [2].
  • This study provides evidence that PH-20 is present on the membrane of canine spermatozoa and in round spermatids [4].
  • For immunolabeling of PH-20 on canine sperm membranes, canine sperm were fixed and incubated with R-10 primary antibody, and an anti-rabbit IgG-FITC secondary antibody [4].

Associations of SPAM1 with chemical compounds

  • Our results thus show that the soluble 60-kDa hyaluronidase from bovine testes is a glycoprotein derived from the sperm PH-20 enzyme [3].
  • The hyaluronidase activity of canine PH-20 was determined with substrate gel electrophoresis based upon digestion of hyaluronic acid (HA) incorporated into the separating gels [4].
  • Canine spermatozoa were extracted with Triton X- 100 and the presence of PH-20 was determined by immunoblot with an antibody against recombinant macaque PH-20 [4].

Analytical, diagnostic and therapeutic context of SPAM1

  • In rat anterior pituitary this factor (denoted SPAM for stimulator of PAM activity) was a soluble protein with a mol wt of 44 K by gel filtration; its stimulatory activity could be reduced or eliminated by trypsin digestion or boiling [2].


  1. JNK1 physically interacts with WW domain-containing oxidoreductase (WOX1) and inhibits WOX1-mediated apoptosis. Chang, N.S., Doherty, J., Ensign, A. J. Biol. Chem. (2003) [Pubmed]
  2. pH-dependent stimulation of peptidylglycine alpha-amidating monooxygenase activity by a granule-associated factor. Perkins, S.N., Husten, E.J., Mains, R.E., Eipper, B.A. Endocrinology (1990) [Pubmed]
  3. The soluble hyaluronidase from bull testes is a fragment of the membrane-bound PH-20 enzyme. Meyer, M.F., Kreil, G., Aschauer, H. FEBS Lett. (1997) [Pubmed]
  4. Characterization of PH-20 in canine spermatozoa and testis. Sabeur, K., Foristall, K., Ball, B.A. Theriogenology (2002) [Pubmed]
  5. Cloning and characterization of the bovine testicular PH-20 hyaluronidase core domain. Chowpongpang, S., Shin, H.S., Kim, E.K. Biotechnol. Lett. (2004) [Pubmed]
WikiGenes - Universities