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Gene Review

ImpL3  -  Ecdysone-inducible gene L3

Drosophila melanogaster

Synonyms: CG10160, Dmel\CG10160, IMP-L3, IMPL3, Imp-L3, ...
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High impact information on ImpL3


Biological context of ImpL3

  • In embryos, IMP-L3 transcript and LDH activity appear in developing somatic muscles by late stage 13, well before the onset of muscular contraction [2].
  • Furthermore, exposure to agonists of ZE1-1 cells expressing the epsilon1/zeta1 NMDA receptor channel resulted in the release of lactate dehydrogenase (LDH) activity in the culture medium indicating agonist-induced cell death [3].
  • Analogous preparations from pupae and adults exhibit three LDH-staining bands (one major and two minor) in a similar pattern [4].
  • This fact indicates that the life-cycle stage-dependent LDH isozymic distribution is possibly due to a posttranslational effect(s) [4].

Anatomical context of ImpL3


Associations of ImpL3 with chemical compounds

  • Out of the nine electrophoretic zones of activity observed on polyacrylamide gel (or out of the six previously observed) using crude extract, only two (one major and one minor) belong to LDH, as revealed by purified enzyme preparations [7].
  • Homogeneous LDH preparations exhibit one band on neutral acrylamide gels when the substrate is either DL-lactic acid or L-(+)-lactate [8].
  • A UEP of 12 is approximately half that of lactate dehydrogenase and shows that aldehyde reductase is evolving at twice the rate of glycolytic enzymes [9].
  • The appearance of a band in an assay using lactic acid as a substrate is not sufficient evidence for the presence of LDH [7].
  • The activities of 2 enzymes (LDH and sMDH) were enhanced after puff induction, an effect that was abolished in the presence of cycloheximide [6].

Analytical, diagnostic and therapeutic context of ImpL3

  • Highly purified LDH from larvae, pupae, or adults, obtained by an affinity chromatography procedure, displays just one dispersed band, located in the area between the band 5 and the band 6 exhibited by crude extract preparations [4].


  1. A conserved family of prolyl-4-hydroxylases that modify HIF. Bruick, R.K., McKnight, S.L. Science (2001) [Pubmed]
  2. IMP-L3, A 20-hydroxyecdysone-responsive gene encodes Drosophila lactate dehydrogenase: structural characterization and developmental studies. Abu-Shumays, R.L., Fristrom, J.W. Dev. Genet. (1997) [Pubmed]
  3. Inducible expression of N-methyl-D-aspartate (NMDA) receptor channels from cloned cDNAs in CHO cells. Uchino, S., Kudo, Y., Watanabe, W., Nakajima-Iijima, S., Mishina, M. Brain Res. Mol. Brain Res. (1997) [Pubmed]
  4. Drosophila lactate dehydrogenase: developmental aspects. Alahiotis, S.N., Onoufriou, A., Fotaki, M., Pelecanos, M. Biochem. Genet. (1983) [Pubmed]
  5. Mutagenicity and dark toxicity of the second-generation photosensitizer bacteriochlorin a. Schuitmaker, J.J., Vogel, E.W., Nagelkerke, J.F., Bos, R.P. J. Photochem. Photobiol. B, Biol. (1998) [Pubmed]
  6. Changes of enzyme activity in larval salivary glands following the induction of respiration dependent puffs in giant chromosomes of Drosophila melanogaster. Behnel, H.J. Cell Differ. (1978) [Pubmed]
  7. Enzyme specificity: "pseudopolymorphism" of lactate dehydrogenase in Drosophila melanogaster. Onoufriou, A., Alahiotis, S.N. Biochem. Genet. (1981) [Pubmed]
  8. Drosophila lactate dehydrogenase: molecular and genetic aspects. Onoufriou, A., Alahiotis, S.N. Biochem. Genet. (1982) [Pubmed]
  9. Evolution of aldehyde reductase: an immunological approach to the relatedness of aldehyde reductase from different species. Davidson, W.S., Flynn, T.G. Adv. Exp. Med. Biol. (1980) [Pubmed]
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