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Ldha  -  lactate dehydrogenase A

Mus musculus

Synonyms: L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, Ldh-1, ...
 
 
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Disease relevance of Ldha

 

High impact information on Ldha

 

Biological context of Ldha

  • Thus, the gene order of Ldh-1 and ru-2 on chromosome 7 remains uncertain [1].
  • These results suggest that either the recombination suppression in the centromere area caused by Rb9 does not extend to the Gpi-1-Ldh-1 genetic region or, if it does, that the differing genetic backgrounds of these two crosses influence recombination [1].
  • The putative initiation sites of transcription and translation were deduced by comparing the nucleotide sequence of mouse LDH-A gene with those of a mouse LDH-A processed pseudogene and the LDH-A full-length cDNAs from rat and human [10].
  • Complete nucleotide sequence of the mouse lactate dehydrogenase-A functional gene: comparison of the exon-intron organization of dehydrogenase genes [11].
  • Thus, mouse LDH-A gene containing seven introns spans approx. 11 kilobases and its length of mature mRNA is 1582 nucleotides, excluding the poly(A) tail [10].
 

Anatomical context of Ldha

  • These data indicate that LDHA, in addition to its function in the metabolism in the glycolytic pathway, may have a novel function in the expression of a cell surface antigen during T cell development [9].
  • LDH-A mRNA levels increased gradually in testes from 16-45-day-old mice [12].
  • In Northern blots, the levels of LDH-A transcripts were low in total testis RNA obtained from 6-12-day-old mice, and in type A and B spermatogonia from 8-day-old mice [12].
  • Sertoli cells were further shown to exhibit comparable amounts of five tetrameric LDH isozymes formed by combination of muscle-type LDH-A and heart-type LDH-B subunits [13].
  • Both LDH-A and LDH-C mRNA levels were low in preleptotene spermatocytes and leptotene/zygotene spermatocytes and increased substantially in pachytene spermatocytes and round spermatids [12].
 

Associations of Ldha with chemical compounds

  • In addition to GAPDHS, ALDOA, and LDHA, this method determined that pyruvate kinase is also tightly bound to the fibrous sheath [14].
  • A mutation affecting the lactate dehydrogenase locus Ldh-1 in the mouse-I. Genetical and electrophoretical characterization [15].
  • The sequence of AAATCTTGCTCAA of mouse LDH-A gene has also been found to show striking homology to the cyclic AMP-responsive sequences of eukaryotic genes regulated by cyclic AMP [10].
  • The exon-intron organization of mouse LDH-A gene is compared with the organizations of other dehydrogenase genes, and the molecular evolution of the nicotinamide adenine dinucleotide binding domains is discussed [11].
  • Four electrophoretic and/or enzyme-activity variants of murine LDH-A subunit (Ldhla-m1Neu, Ldhla-m5Neu, Ldhla-m6Neu, Ldhla-m9Neu), induced by procarbazine hydrochloride or ethylnitrosourea (ENU), were analyzed at the DNA level [16].
 

Other interactions of Ldha

 

Analytical, diagnostic and therapeutic context of Ldha

  • Flow cytometry analysis of thymocyte subpopulations showed that some thymocytes at the CD4(-)CD8(-) double negative stage express both IMT-1 and LDHA on their surface [9].
  • Immunoprecipitation analysis revealed that LDHA could be co-precipitated with IMT-1 from the cell surface of EL4 cells that had been transfected with LDHA cDNA, suggesting that some of LDHA is associated with cell surface IMT-1 on the transfectants [9].
  • Exons of the Ldh1 gene were PCR amplified and sequenced to determine the molecular lesion in the mutant alleles [20].
  • In situ hybridization mapping of LDHA and IGF2 to cattle chromosome 29 [21].
  • This included isoelectric focusing of purified LDH A, B, and X, two-dimensional separation of LDH, urea treatment of LDH, staining with specific substrates, and comparison of different organs [22].

References

  1. Analysis of recombination in the centromere region of mouse chromosome 7 using ovarian teratoma and backcross methods. Eppig, J.T., Eicher, E.M. J. Hered. (1988) [Pubmed]
  2. A mutation affecting the lactate dehydrogenase locus Ldh-1 in the mouse. II. Mechanism of the LDH-A deficiency associated with hemolytic anemia. Pretsch, W., Merkle, S., Favor, J., Werner, T. Genetics (1993) [Pubmed]
  3. Assignment of the gene for lactic dehydrogenase A to mouse chromosome 7 using mouse-human hybrids. O'Brien, D., Linnenbach, A., Croce, C.M. Cytogenet. Cell Genet. (1978) [Pubmed]
  4. Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl2 in Qinghai-Tibetan plateau mammals and sea level mice. Chen, X.Q., Wang, S.J., Du, J.Z., Chen, X.C. Am. J. Physiol. Regul. Integr. Comp. Physiol. (2007) [Pubmed]
  5. Expression of the mouse lactate dehydrogenase-A promoter fused with the bacterial gpt gene in Chinese hamster ovary cells. Hou, E.W., Tindall, K.R., Li, S.S. Int. J. Biochem. (1988) [Pubmed]
  6. Rhabdomyosarcoma-associated locus and MYOD1 are syntenic but separate loci on the short arm of human chromosome 11. Scrable, H.J., Johnson, D.K., Rinchik, E.M., Cavenee, W.K. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  7. Tumor induction by the c-Myc target genes rcl and lactate dehydrogenase A. Lewis, B.C., Prescott, J.E., Campbell, S.E., Shim, H., Orlowski, R.Z., Dang, C.V. Cancer Res. (2000) [Pubmed]
  8. Transgenic mice demonstrate a testis-specific promoter for lactate dehydrogenase, LDHC. Li, S., Zhou, W., Doglio, L., Goldberg, E. J. Biol. Chem. (1998) [Pubmed]
  9. Lactate dehydrogenase A-dependent surface expression of immature thymocyte antigen-1: an implication for a novel trafficking function of lactate dehydrogenase-A during T cell development. Fujishiro, Y., Kishi, H., Matsuda, T., Fuse, H., Muraguchi, A. Eur. J. Immunol. (2000) [Pubmed]
  10. Nucleotide sequence of the putative regulatory region of mouse lactate dehydrogenase-A gene. Fukasawa, K.M., Li, S.S. Biochem. J. (1986) [Pubmed]
  11. Complete nucleotide sequence of the mouse lactate dehydrogenase-A functional gene: comparison of the exon-intron organization of dehydrogenase genes. Fukasawa, K.M., Li, S.S. Genetics (1987) [Pubmed]
  12. DNA methylation and expression of the genes coding for lactate dehydrogenases A and C during rodent spermatogenesis. Alcivar, A.A., Trasler, J.M., Hake, L.E., Salehi-Ashtiani, K., Goldberg, E., Hecht, N.B. Biol. Reprod. (1991) [Pubmed]
  13. Differential activity and synthesis of lactate dehydrogenase isozymes A (muscle), B (heart), and C (testis) in mouse spermatogenic cells. Li, S.S., O'Brien, D.A., Hou, E.W., Versola, J., Rockett, D.L., Eddy, E.M. Biol. Reprod. (1989) [Pubmed]
  14. Multiple glycolytic enzymes are tightly bound to the fibrous sheath of mouse spermatozoa. Krisfalusi, M., Miki, K., Magyar, P.L., O'Brien, D.A. Biol. Reprod. (2006) [Pubmed]
  15. A mutation affecting the lactate dehydrogenase locus Ldh-1 in the mouse-I. Genetical and electrophoretical characterization. Charles, D.J., Pretsch, W. Biochem. Genet. (1981) [Pubmed]
  16. Molecular analysis of four lactate dehydrogenase-A mutants in the mouse. Sandulache, R., Pretsch, W., Chatterjee, B., Gimbel, W., Graw, J., Favor, J. Mamm. Genome (1994) [Pubmed]
  17. Abundance of repetitive sequence elements in the mouse testis-specific lactate dehydrogenase-C gene. Olsson, P.G., Tsujioka, H., Narisawa, S., Goldberg, E., Millán, J.L. J. Androl. (2003) [Pubmed]
  18. TEM-induced gene mutations at enzyme loci in the mouse. Soares, E.R. Environmental mutagenesis. (1979) [Pubmed]
  19. Cyclic AMP-induced expression of the mouse lactate dehydrogenase-A promoter-cat fusion gene in Chinese hamster ovary wild-type cells, but not in cAMP-dependent protein kinase mutant cells. Hou, E.W., Li, S.S. Biochem. Biophys. Res. Commun. (1987) [Pubmed]
  20. Molecular, genetic and biochemical characterization of lactate dehydrogenase-A enzyme activity mutations in Mus musculus. Pretsch, W., Chatterjee, B., Favor, J., Merkle, S., Sandulache, R. Mamm. Genome (1998) [Pubmed]
  21. In situ hybridization mapping of LDHA and IGF2 to cattle chromosome 29. Schmutz, S.M., Moker, J.S., Gallagher, D.S., Kappes, S.M., Womack, J.E. Mamm. Genome (1996) [Pubmed]
  22. Gel isoelectric focusing of mouse lactate dehydrogenase: heterogeneity of the isoenzymes A4 and X4. Klose, J., Spielmann, H. Biochem. Genet. (1975) [Pubmed]
 
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