High impact information on PLLP

• Sequence analysis of plasmolipin cDNA shows an open reading frame encoding a 157-amino acid protein of 17.4 kDa [1].
• Northern blot analysis 1) shows that plasmolipin is encoded by a 1.7-kilobase mRNA, 2) confirms that the distribution of plasmolipin is restricted to kidney and brain, and 3) indicates that the expression of plasmolipin mRNA in cultured oligodendrocytes increases with cell maturation consistent with changes in the level of the protein [1].
• Presence of the plasma membrane proteolipid (plasmolipin) in myelin [2].
• In this study, we examined the distribution of plasmolipin in brain regions, myelin, and oligodendroglial membranes [2].
• When myelin or white matter was extracted with chloroform/methanol, it contained, in addition to PLP, a significant amount of plasmolipin [2].

Biological context of PLLP

• We have cloned and sequenced the mouse plasmolipin gene, revealing the common organization of the 4TM gene group with four exons and a large first intron [3].
• With respect to intrafamiliar variations in the manifestation of BBS, we suggest that plasmolipin might be either another candidate gene or a modifier of the BBS2 phenotype [3].
• By means of radiation hybrid mapping and FISH analysis, we could localize the human plasmolipin gene to Chromosome 16q13 within the putative region of the Bardet-Biedl syndrome type 2 (BBS2) gene locus [3].

Anatomical context of PLLP

• Plasmolipin is a membrane protein and belongs to the tetraspan molecule (4TM) family, an expanding group of myelin proteins many of which could be linked to human hereditary demyelinating neuropathies [3].
• Western blot analysis with an antibody raised against the C-terminal intracellular part of the protein showed that plasmolipin is expressed not only in the nervous system and kidney, but also in a number of other tissues such as thymus, testis, lung, and thyroid gland [3].
• Removal of the clathrin coat proteins or exposure of the coated vesicles to 0.1 M Na2CO3 showed that the plasma membrane proteolipid protein is not removed during uncoating and lysis but is intrinsic to the membrane bilayer of these vesicles [4].
• We have analyzed brain coated vesicles and synaptic plasma membrane for the presence of the plasma membrane proteolipid protein [4].
• Comparison of the central and peripheral nervous systems of mammals showed only minor differences in the level of plasmolipin in these two regions [5].

Other interactions of PLLP

• CKLFSF8 shares 39.3% amino acid identity with TM4SF11 [6].
• Plasmolipin: the other myelin proteolipid. A review of studies on its structure, expression, and function [7].

Analytical, diagnostic and therapeutic context of PLLP

• Restriction enzyme digestion of DNA followed by Southern blot analysis indicates that plasmolipin is encoded by a single gene [1].
• Isolation of synaptic plasma membranes proteolipid adult rat brain and estimation of the plasma membrane proteolipid protein content using the immunoblotting method confirmed earlier studies that show this protein is present in this membrane fraction at high levels as well (approximately 1-2%) [4].

References