Disease relevance of Pycard

• A comparison of the initial rates of aminoacylation of tRNAs isolated from different sources for 17 amino acids was performed. tRNA was isolated from NMRI mouse liver (tRNA L) and from Krebs II ascites tumors (tRNA Asc), and aminoacyl-tRNA synthetases were prepared from the latter cells [1].
• Extracted antigen (Asc) and eggs that had been killed also were able to induce marked eosinophilia; however, their levels were significantly lower (p less than 0.001) [2].

High impact information on Pycard

• T cells primed with Asc in CFA showed helper function mainly on IgG antibody response but not on IgE antibody response [3].
• Carrier-specific T cells induced with a low dose of Asc (0.01 mug) showed helper function only on IgE antibody response, whereas T cells primed with a higher dose of Asc (10 mug) cooperated only with IgG-B cells [3].
• At E9.5, mASC was strongly expressed in the telencephalon, thalamic areas of the diencephalon, heart, and liver [4].
• We have cloned a murine ortholog of ASC (mASC) comprising 193 amino acids with a well-conserved pyrin N-terminal homology domain and caspase recruitment domain (CARD). mASC fused with green fluorescent protein appeared as a speck in transfected COS-7 cells and showed self-association [4].
• We analyzed mASC gene expression in developing embryos by in situ hybridization and found it to have a restricted distribution in mouse embryos [4].

Biological context of Pycard

• The aminoacylation of tRNA Asc was 31-88% slower than the charging of tRNA L [1].

Anatomical context of Pycard

• Upon stimulation with LPS, Asc(-/-) macrophages failed in the processing of procaspase-1 and maturation of pro-IL-1beta and pro-IL-18, but normally produced other pro-inflammatory cytokines including TNF-alpha and IL-6 [5].
• It is possible to measure Asc.- easily using ESR and a dialysis method in which Asc.- was collected at room temperature in the interstitial fluid through the dialysis membrane [6].
• To study the effect of antigenic competition on T and B cells, donor mice of one or the other cell type received in addition the competitor antigen (Asc) at immunization [7].

Associations of Pycard with chemical compounds

• These results suggest that the amount of HO. and O2.- produced is reflected in the Asc.- production [6].
• The addition of ferrous ions and ascorbic acid (Fe2+/ Asc) to mouse sperm suspensions increases the formation of thiobarbituric acid-reactive substances (TBARS), an indicator of lipid peroxide breakdown products, without significantly affecting the level of sulfhydryl groups [8].
• Also, the increase in Asc.- production when WR-2721 was administered prior to irradiation was less than for experiments in which irradiation only was performed [6].
• Asc.- increased after treatment with the agents H2O2 and FeCl2, while it decreased after treatment with SOD and catalase [6].

Analytical, diagnostic and therapeutic context of Pycard

• Northern blotting analysis revealed that the mASC gene was expressed ubiquitously in multiple organs in adult mice [4].

References