Disease relevance of ZNF354A

• 3. Since the chromosome 5q22-qter region is duplicated in approximately 50% of conventional renal-cell carcinomas, it was suggested that Tcf17 is involved in the development of renal tumors [1].
• TCF17 mRNA levels are high in carcinoma in situ and in seminoma, a tumor derived from carcinoma in situ but still of low-grade malignancy [2].
• TCF17, the human homologue of the rat zinc finger gene Kid1, is highly expressed in neurons derived from the retinoic acid-treated human embryonal carcinoma (EC) cell line, NTERA-2 [2].

High impact information on ZNF354A

• Differential expression of the zinc finger gene TCF17 in testicular tumors [2].
• This differentiation-related up-regulation of TCF17 prompted us to investigate its expression during human spermatogenesis and in human testicular germ cell tumors considered to be precursors of EC [2].
• The differential regulation of TCF17 during neoplastic germ cell differentiation may be of predictive value in germ cell tumor diagnosis [2].
• Expression of TCF17 increases as spermatogonia differentiate into spermatocytes, indicating that this gene is developmentally regulated during spermatogenesis [2].
• The tcf17 gene at chromosome 5q is not involved in the development of conventional renal cell carcinoma [1].

Biological context of ZNF354A

• We found ubiquitous expression of human Tcf17 as well as rat Kid-1 in different types of human and rat tissue, indicating that the putative transcription-regulating activity of this zinc finger gene, in contrast to the published data, is not restricted to the kidney [1].
• The human Kid-1 homolog Tcf17 has been cloned and assigned to chromosome 5q35 [1].
• The deduced amino acid sequence showed 86.0% and 87.2% identity (91.6% and 92.8% similarity) with the rat Kid1 and mouse Tcf17 respectively [3].
• KID1 is a putative transcriptional repressor, containing a KRAB domain at its amino terminus that mediates transcriptional repression in transient cell transfection assays when fused to a heterologous DNA-binding domain [4].

Associations of ZNF354A with chemical compounds

• DNA sequence analysis revealed that HKL-1 clone was 448 bp long, and its putative amino acid sequence was identical with that of a human cysteine proteinase inhibitor, cystatin A. Western blot analysis showed that the commercially available recombinant cystatin A also reacted specifically with the anti-human keratolinin antibody [5].

Analytical, diagnostic and therapeutic context of ZNF354A

• Northern blot analysis indicated that HKL-1 clone hybridizes with mRNA of about 0.5 kb, consistent with the size of the HKL-1 clone [5].

References