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Gene Review

UBE2B  -  ubiquitin-conjugating enzyme E2B

Homo sapiens

Synonyms: E2-17kDa, HHR6B, HR6B, RAD6 homolog B, RAD6B, ...
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Disease relevance of UBE2B

  • Whereas MCF10A cells engineered to overexpress ectopic RAD6B are significantly more resistant to ADR and cisplatin as compared to empty vector-transfected cells, MCF10A cells stably transfected with antisense RAD6B display hypersensitivity to these damage-inducing drugs [1].
  • Since heterozygous male mice and even knockout female mice are completely normal and fertile and thus able to transmit the defect, similar hHR6B mutations may cause male infertility in man [2].

High impact information on UBE2B

  • We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B [3].
  • Constitutive overexpression of exogenous human HR6B cDNA into normal-behaving MCF10A human breast epithelial cells induced cell-cell fusion that resulted in generation of multinucleated cells, centrosome amplification, multipolar mitotic spindles, aneuploidy, and ability for anchorage-independent growth [4].
  • A survey of E1 and E2 isoform distribution in selected cell lines demonstrates that Ubc2 isoforms are the predominant intracellular ubiquitin carrier protein [5].
  • Inclusion of actinomycin D abolishes both basal and ADR-induced RAD6B transcription indicating that ADR-induced effects on RAD6B transcription result from an increase in transcriptional activity rather than from regulation of RAD6B mRNA stability [1].
  • This review attempts to integrate recent knowledge regarding flagellar components that could be related to proteasome components and, therefore, could be targets of UBE2B in the spermatid [6].

Biological context of UBE2B


Associations of UBE2B with chemical compounds

  • Exposure of MCF10A cells to adriamycin (ADR) causes enhancement in the levels of RAD6B mRNA and protein [1].

Other interactions of UBE2B


Analytical, diagnostic and therapeutic context of UBE2B

  • Southern blot analysis points to an X and an autosomal localization of HHR6A and HHR6B, respectively, in the mouse [7].


  1. RAD6B overexpression confers chemoresistance: RAD6 expression during cell cycle and its redistribution to chromatin during DNA damage-induced response. Lyakhovich, A., Shekhar, M.P. Oncogene (2004) [Pubmed]
  2. Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification. Roest, H.P., van Klaveren, J., de Wit, J., van Gurp, C.G., Koken, M.H., Vermey, M., van Roijen, J.H., Hoogerbrugge, J.W., Vreeburg, J.T., Baarends, W.M., Bootsma, D., Grootegoed, J.A., Hoeijmakers, J.H. Cell (1996) [Pubmed]
  3. Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6. Koken, M.H., Reynolds, P., Jaspers-Dekker, I., Prakash, L., Prakash, S., Bootsma, D., Hoeijmakers, J.H. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  4. Rad6 overexpression induces multinucleation, centrosome amplification, abnormal mitosis, aneuploidy, and transformation. Shekhar, M.P., Lyakhovich, A., Visscher, D.W., Heng, H., Kondrat, N. Cancer Res. (2002) [Pubmed]
  5. Protein interactions within the N-end rule ubiquitin ligation pathway. Siepmann, T.J., Bohnsack, R.N., Tokgöz, Z., Baboshina, O.V., Haas, A.L. J. Biol. Chem. (2003) [Pubmed]
  6. New insights into the assembly of the periaxonemal structures in mammalian spermatozoa. Escalier, D. Biol. Reprod. (2003) [Pubmed]
  7. Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31. Koken, M.H., Smit, E.M., Jaspers-Dekker, I., Oostra, B.A., Hagemeijer, A., Bootsma, D., Hoeijmakers, J.H. Genomics (1992) [Pubmed]
  8. Molecular cloning and characterization of cDNA encoding a ubiquitin-conjugating enzyme from Clonorchis sinensis. Song, L., Chen, S., Yu, X., Wu, Z., Xu, J., Yang, G., Zheng, N., Hu, X., Guo, L., Dai, J., Xu, J., Ji, C., Gu, S., Ying, K. Parasitol. Res. (2004) [Pubmed]
  9. Specific aspects of the ubiquitin system in spermatogenesis. Baarends, W.M., van der Laan, R., Grootegoed, J.A. J. Endocrinol. Invest. (2000) [Pubmed]
  10. (CGG) trinucleotide repeat polymorphism in the 5' region of the HHR6B gene: the human homolog of the yeast DNA repair gene RAD6. Lench, N.J., Thompson, J., Markham, A.F., Robinson, P.A. Hum. Genet. (1995) [Pubmed]
  11. The human RAD18 gene product interacts with HHR6A and HHR6B. Xin, H., Lin, W., Sumanasekera, W., Zhang, Y., Wu, X., Wang, Z. Nucleic Acids Res. (2000) [Pubmed]
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