Disease relevance of ZNF85

• Here, we show that the ZNF85 gene is highly expressed in normal adult testis, in seminomas, and in the NT2/D1 teratocarcinoma cell line [1].
• In contrast, 6B4 was a weak inhibitor in this ex-vivo thrombosis model, and HTF1 displayed no inhibition at all [2].
• However, all 22 patients who had thromboembolism had HPF4/A [3].

High impact information on ZNF85

• Rapid detection of heparin-induced platelet antibodies with particle gel immunoassay (ID-HPF4) [4].
• The common epitope of the antibodies 6B4 and HTF1 included residues Tyr94 and Phe76 both of which make critical contacts to the catalytic domain of F.VIIa [2].
• A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements cAMP responsive element, HTF-1, and acute phase signal sequences [5].
• Heparin-induced platelet aggregation, C-serotonin release assay, and enzyme-linked immmunosorbent assay (ELISA) tests were performed in all the patients to detect the antibodies formed against the complex of heparin and platelet factor 4 (HPF4) [6].
• HIT II was diagnosed clinically; heparin-induced platelet activation test (HIPA) and serum IgG, IgA, and IgM to heparin-platelet factor 4 complexes (HPF4) were both negative [7].

Chemical compound and disease context of ZNF85

• We analyzed the occurrence of HPF4/A and thromboembolism in patients with heparin-coated and noncoated VADs [3].

Biological context of ZNF85

• Functional analysis of ZNF85 KRAB zinc finger protein, a member of the highly homologous ZNF91 family [1].
• Generation and expression of the autoantigen, HPF4 complexes, is a key feature for triggering antibody binding and pathogenicity [8].

Associations of ZNF85 with chemical compounds

• We have examined the effects of seven proteases on human placental tissue factor in Triton X-100, focusing on extracellular and cytoplasmic domains recognized by monoclonal antibodies HTF1, C28 1.1, and C28 2 [9].

Analytical, diagnostic and therapeutic context of ZNF85

• HPF4/A testing was performed before and 2 and 12 weeks after implantation by the heparin platelet factor 4 enzyme-linked immunosorbent assay [3].
• 1. Plasmin produced peptides recognized on Western blots by C281.1 but not HTF1 [9].

References