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Gene Review:

CBF2 - dehydration-responsive element-binding...

Arabidopsis thaliana

Synonyms: ATCBF2, C-repeat/DRE binding factor 2, DRE/CRT-BINDING PROTEIN, DRE/CRT-BINDING PROTEIN 1C, DREB1C, ...

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Disease relevance of CBF2

Here, we show that cbf2 plants have higher capacity to tolerate freezing than WT ones before and after cold acclimation and are more tolerant to dehydration and salt stress [1].

High impact information on CBF2

The transcript levels of approximately 8000 genes were determined at multiple times after plants were transferred from warm to cold temperature and in warm-grown plants that constitutively expressed CBF1, CBF2, or CBF3 [2].
An analysis of CBF2 promoter-reporter gene fusions indicated that this control included transcriptional regulation [3].
Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis [4].
The low freezing tolerance of FTQ4-Cvi alleles was associated with a deletion of the promoter region of Cvi CBF2, and with low RNA expression of CBF2 and of several CBF target genes [4].
Of the 25 core cold-induced genes that were most highly upregulated (induced over 15-fold), 19 genes (84%) were induced by CBF2 and another two genes (8%) were regulated by both CBF2 and ZAT12 [5].

Biological context of CBF2

Taken together, all of these data suggest that CBF2 and CBF3 may function as transcriptional activators, controlling the level of low-temperature gene expression and promoting freezing tolerance through an abscisic acid-independent pathway [6].
Like CBF1, both CBF2 and CBF3 activated expression of reporter genes in yeast that contained the CRT/DRE as an upstream activator sequence [7].
The CBF2 and CBF3 genes are linked to CBF1, constituting a cluster on the bottom arm of chromosome IV [6].
A quantification of mRNA levels in leaves of parental and F(1) lines using quantitative real-time RT-PCR showed no clear indication for an involvement of the investigated genes (CBF (C-repeat binding factor)1, CBF2, (cold-regulated protein (COR) 6.6, COR15a, COR15b, COR47 and COR78) in the heterosis effect [8].
Genetic complementation of FTQ4-Cvi plants with a CBF2-Ler transgene suggests that such CBF2 allelic variation is the cause of CBF2 misexpression and the molecular basis of FTQ4 [4].

Associations of CBF2 with chemical compounds

A total of 125 bp of the CBF2 promoter, which has previously been shown to be sufficient for cold-, mechanical-, and cycloheximide-induced expression, was also sufficient for ABA-induced expression [9].

Other interactions of CBF2

Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis [5].

References

CBF2/DREB1C is a negative regulator of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in stress tolerance in Arabidopsis. Novillo, F., Alonso, J.M., Ecker, J.R., Salinas, J. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Fowler, S., Thomashow, M.F. Plant Cell (2002) [Pubmed]
Low temperature induction of Arabidopsis CBF1, 2, and 3 is gated by the circadian clock. Fowler, S.G., Cook, D., Thomashow, M.F. Plant Physiol. (2005) [Pubmed]
Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis. Alonso-Blanco, C., Gomez-Mena, C., Llorente, F., Koornneef, M., Salinas, J., Martínez-Zapater, J.M. Plant Physiol. (2005) [Pubmed]
Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis. Vogel, J.T., Zarka, D.G., Van Buskirk, H.A., Fowler, S.G., Thomashow, M.F. Plant J. (2005) [Pubmed]
The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression Is regulated by low temperature but not by abscisic acid or dehydration. Medina, J., Bargues, M., Terol, J., Pérez-Alonso, M., Salinas, J. Plant Physiol. (1999) [Pubmed]
Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Gilmour, S.J., Zarka, D.G., Stockinger, E.J., Salazar, M.P., Houghton, J.M., Thomashow, M.F. Plant J. (1998) [Pubmed]
Heterosis in the freezing tolerance of crosses between two Arabidopsis thaliana accessions (Columbia-0 and C24) that show differences in non-acclimated and acclimated freezing tolerance. Rohde, P., Hincha, D.K., Heyer, A.G. Plant J. (2004) [Pubmed]
Abscisic acid induces CBF gene transcription and subsequent induction of cold-regulated genes via the CRT promoter element. Knight, H., Zarka, D.G., Okamoto, H., Thomashow, M.F., Knight, M.R. Plant Physiol. (2004) [Pubmed]

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