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Gene Review

GUS3  -  glucuronidase 3

Arabidopsis thaliana

Synonyms: AtGUS3, T2L5.6
 
 
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Disease relevance of AT5G34940

  • To gain a better understanding of the regulatory mechanism of plant metallothionein (MT) genes, a chimeric expression unit consisting of the beta-glucuronidase (gusA) reporter gene under the control of a 1,324 bp fragment of the rice MT (ricMT) promoter was introduced into Arabidopsis via Agrobacterium tumefaciens [1].
 

High impact information on AT5G34940

  • To identify mutants involved in the regulation of PR genes and the onset of SAR, we transformed Arabidopsis with a reporter gene containing the promoter of a beta-1,3-glucanase-encoding PR gene (BGL2) and the coding region of beta-glucuronidase (GUS) [2].
  • An AtAmt1.1-Gal4 transgene using three 5x upstream activating sequence-driven reporters (luciferase, green fluorescent protein, and beta-glucuronidase) facilitated in vivo profiling at the whole-plant and cellular levels [3].
  • To analyze DSB repair, we used a homologous recombination (HR) and point mutation reversion assays based on nonfunctional beta-glucuronidase reporter genes [4].
  • Initially, we used the ABA-responsive Em promoter from wheat linked to beta-glucuronidase (GUS) to determine whether ABI3/VP1, transcriptional regulators in the ABA-signaling pathway in angiosperms, were similarly active in the ABA response of P. patens [5].
  • On fusion with beta-glucuronidase (GUS), the C-terminal domain of either OsCRY1a (OsCCT1a) or OsCRY1b (OsCCT1b) mediated a constitutive photomorphogenic (COP) phenotype in both Arabidopsis and rice, whereas OsCCT1b mutants corresponding to missense mutations in previously described Arabidopsis cry1 alleles failed to confer a COP phenotype [6].
 

Biological context of AT5G34940

  • Light microscopy analysis revealed that the beta-glucuronidase from the enhancer trap was expressed in a seven-layer zone, encompassing the valve margin and the replum border [7].
  • Furthermore we show that blue and far-red light can suppress the ploidy increase in ipd1-1D and also suppress the reporter expression in IPD1-promoter beta-glucuronidase transgenic plants [8].
  • Homologous recombination events were detected in transgenic Arabidopsis plants that carried in their genome a beta-glucuronidase recombination marker [9].
  • The fragment was used to substitute the 35S promoter of the pBI121 plasmid to construct a beta-glucuronidase gene (GUS) expression system [10].
  • Promoter analysis of the sesame FAD2 gene (SeFAD2) using the beta-glucuronidase (GUS) reporter system demonstrated that the - 660 to - 180 promoter region functions as a negative cis-element in the seed-specific expression of the SeFAD2 gene [11].
 

Associations of AT5G34940 with chemical compounds

  • Transcripts for beta-glucuronidase (GUS) driven by BiP promoter respond to tunicamycin and sugar, being similar with endogenous BiP transcripts in transgenic A. thaliana [12].
  • Estrogen-dependent interaction between the two effector proteins triggers transcriptional activation of reporter gene, beta-glucuronidase [13].
  • The AtNMT1 uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream beta-glucuronidase gene translation in the calli when the cells were treated with choline [14].

References

  1. The GUS reporter-aided analysis of the promoter activities of a rice metallothionein gene reveals different regulatory regions responsible for tissue-specific and inducible expression in transgenic Arabidopsis. Lü, S., Gu, H., Yuan, X., Wang, X., Wu, A.M., Qu, L., Liu, J.Y. Transgenic Res. (2007) [Pubmed]
  2. A mutation in Arabidopsis that leads to constitutive expression of systemic acquired resistance. Bowling, S.A., Guo, A., Cao, H., Gordon, A.S., Klessig, D.F., Dong, X. Plant Cell (1994) [Pubmed]
  3. Reciprocal Leaf and Root Expression of AtAmt1.1 and Root Architectural Changes in Response to Nitrogen Starvation. Engineer, C.B., Kranz, R.G. Plant Physiol. (2007) [Pubmed]
  4. Double-strand break repair in plants is developmentally regulated. Boyko, A., Zemp, F., Filkowski, J., Kovalchuk, I. Plant Physiol. (2006) [Pubmed]
  5. Characterization and functional analysis of ABSCISIC ACID INSENSITIVE3-like genes from Physcomitrella patens. Marella, H.H., Sakata, Y., Quatrano, R.S. Plant J. (2006) [Pubmed]
  6. Functional and signaling mechanism analysis of rice CRYPTOCHROME 1. Zhang, Y.C., Gong, S.F., Li, Q.H., Sang, Y., Yang, H.Q. Plant J. (2006) [Pubmed]
  7. The INDEHISCENT protein regulates unequal cell divisions in Arabidopsis fruit. Wu, H., Mori, A., Jiang, X., Wang, Y., Yang, M. Planta (2006) [Pubmed]
  8. Light-dependent polyploidy control by a CUE protein variant in Arabidopsis. Tsumoto, Y., Yoshizumi, T., Kuroda, H., Kawashima, M., Ichikawa, T., Nakazawa, M., Yamamoto, N., Matsui, M. Plant Mol. Biol. (2006) [Pubmed]
  9. Increase of homologous recombination frequency in vascular tissue of Arabidopsis plants exposed to salt stress. Boyko, A., Hudson, D., Bhomkar, P., Kathiria, P., Kovalchuk, I. Plant Cell Physiol. (2006) [Pubmed]
  10. Expression of NAC1 up-stream regulatory region and its relationship to the lateral root initiation induced by gibberellins and auxins. Wang, Y., Duan, L., Lu, M., Li, Z., Wang, M., Zhai, Z. Sci. China, C, Life Sci. (2006) [Pubmed]
  11. Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the SeFAD2 promoter and enhancers in the 5'-UTR intron. Kim, M.J., Kim, H., Shin, J.S., Chung, C.H., Ohlrogge, J.B., Suh, M.C. Mol. Genet. Genomics (2006) [Pubmed]
  12. Induction of BiP by sugar independent of a cis-element for the unfolded protein response in Arabidopsis thaliana. Tajima, H., Koizumi, N. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
  13. A simple and extremely sensitive system for detecting estrogenic activity using transgenic Arabidopsis thaliana. Tojo, T., Tsuda, K., Wada, T.S., Yamazaki, K. Ecotoxicol. Environ. Saf. (2006) [Pubmed]
  14. Posttranscriptional regulation by the upstream open reading frame of the phosphoethanolamine N-methyltransferase gene. Tabuchi, T., Okada, T., Azuma, T., Nanmori, T., Yasuda, T. Biosci. Biotechnol. Biochem. (2006) [Pubmed]
 
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