Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

RDR1 - RNA-dependent RNA polymerase 1

Arabidopsis thaliana

Synonyms: ATRDRP1, AtRDR1, F10B6.19, F10B6_19

Jakubiec, A. et al., Tang, G. et al., Dalmay, T. et al., Li, H. et al., Nakayashiki, H. et al., et al.

Nakayashiki, Matsuo, Chuma, Ikeda, Betsuyaku, Kusaba, Tosa, Mayama,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of RDR1

Here we show that Turnip yellow mosaic virus (TYMV) RdRp is phosphorylated, when expressed both individually and in the context of viral infection [1].
Phosphorylation of viral RNA-dependent RNA polymerase and its role in replication of a plus-strand RNA virus [1].
A WCCH motif-like sequence is found in the precoat protein of some geminiviruses, viral RNA-dependent RNA polymerase and also in an Arabidopsis protein of unknown function [2].

High impact information on RDR1

One of these, SDE1, is a plant homolog of QDE-1 in Neurospora crassa that encodes an RNA-dependent RNA polymerase [3].
An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus [3].
We find that standard wheat germ extract contains Dicer-like enzymes that convert double-stranded RNA (dsRNA) into two classes of small interfering RNAs, as well as an RNA-dependent RNA polymerase activity that can convert exogenous single-stranded RNA into dsRNA [4].
MicroRNA-targeted and small interfering RNA-mediated mRNA degradation is regulated by argonaute, dicer, and RNA-dependent RNA polymerase in Arabidopsis [5].
The Putative RNA-dependent RNA polymerase RDR6 acts synergistically with ASYMMETRIC LEAVES1 and 2 to repress BREVIPEDICELLUS and MicroRNA165/166 in Arabidopsis leaf development [6].

Biological context of RDR1

It also was dependent on transcription of the transgene and on the putative RNA-dependent RNA polymerase, SDE1/SGS2 [7].
Our results also highlighted the role of another TYMV-encoded replication protein as an antagonistic protein that may prevent the inhibitory effect of RdRp phosphorylation on viral infectivity [1].
Based on these data, we propose that phosphorylation-dependent regulatory mechanisms are essential for viral RdRp function and virus replication [1].
Central to the process of plus-strand RNA virus genome amplification is the viral RNA-dependent RNA polymerase (RdRp) [1].
Identification of trans-acting siRNAs in moss and an RNA-dependent RNA polymerase required for their biogenesis [8].

Other interactions of RDR1

Analysis of the involvement of an inducible Arabidopsis RNA-dependent RNA polymerase in antiviral defense [9].

References

Phosphorylation of viral RNA-dependent RNA polymerase and its role in replication of a plus-strand RNA virus. Jakubiec, A., Tournier, V., Drugeon, G., Pflieger, S., Camborde, L., Vinh, J., Héricourt, F., Redeker, V., Jupin, I. J. Biol. Chem. (2006) [Pubmed]
Pyret, a Ty3/Gypsy retrotransposon in Magnaporthe grisea contains an extra domain between the nucleocapsid and protease domains. Nakayashiki, H., Matsuo, H., Chuma, I., Ikeda, K., Betsuyaku, S., Kusaba, M., Tosa, Y., Mayama, S. Nucleic Acids Res. (2001) [Pubmed]
An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Dalmay, T., Hamilton, A., Rudd, S., Angell, S., Baulcombe, D.C. Cell (2000) [Pubmed]
A biochemical framework for RNA silencing in plants. Tang, G., Reinhart, B.J., Bartel, D.P., Zamore, P.D. Genes Dev. (2003) [Pubmed]
MicroRNA-targeted and small interfering RNA-mediated mRNA degradation is regulated by argonaute, dicer, and RNA-dependent RNA polymerase in Arabidopsis. Ronemus, M., Vaughn, M.W., Martienssen, R.A. Plant Cell (2006) [Pubmed]
The Putative RNA-dependent RNA polymerase RDR6 acts synergistically with ASYMMETRIC LEAVES1 and 2 to repress BREVIPEDICELLUS and MicroRNA165/166 in Arabidopsis leaf development. Li, H., Xu, L., Wang, H., Yuan, Z., Cao, X., Yang, Z., Zhang, D., Xu, Y., Huang, H. Plant Cell (2005) [Pubmed]
Spreading of RNA targeting and DNA methylation in RNA silencing requires transcription of the target gene and a putative RNA-dependent RNA polymerase. Vaistij, F.E., Jones, L., Baulcombe, D.C. Plant Cell (2002) [Pubmed]
Identification of trans-acting siRNAs in moss and an RNA-dependent RNA polymerase required for their biogenesis. Talmor-Neiman, M., Stav, R., Klipcan, L., Buxdorf, K., Baulcombe, D.C., Arazi, T. Plant J. (2006) [Pubmed]
Analysis of the involvement of an inducible Arabidopsis RNA-dependent RNA polymerase in antiviral defense. Yu, D., Fan, B., MacFarlane, S.A., Chen, Z. Mol. Plant Microbe Interact. (2003) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

RDR2	Arabidopsis thaliana
RDR6	Arabidopsis thaliana
MGG_02748	Magnaporthe oryzae 70-15
NCU02178	Neurospora crassa OR74A
Os02g0736200	Oryza sativa Japonica Group
rdp1	Schizosaccharomyces pombe 972h-

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.