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Gene Review

ROM2  -  Rom2p

Saccharomyces cerevisiae S288c

Synonyms: L8039.3, RHO1 GDP-GTP exchange protein 2, YLR371W
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High impact information on ROM2

  • TOR2 activates this switch via ROM2, possibly via the ROM2 PH domain [1].
  • Rom2p, the GDP/GTP exchange factor of Rho1p, is preferentially localized on the plasma membrane even when vesicular transport is blocked [2].
  • Overexpression of Rom2p results in delocalization of Rom2p and accumulation of 1,3-beta-glucan in secretory vesicles [2].
  • Rom1p and Rom2p were found to contain a DH (Dbl homologous) domain and a PH (pleckstrin homologous) domain, both of which are conserved among the GDP/GTP exchange proteins (GEPs) for the Rho family small GTP binding proteins [3].
  • The glutathione-S-transferase (GST) fusion protein containing the DH domain of Rom2p showed the lipid-modified Rholp-specific GDP/GTP exchange activity which was sensitive to Rho GDP dissociation inhibitor [3].

Biological context of ROM2

  • Disruption of ROM2 resulted in a temperature-sensitive growth phenotype, whereas disruption of both ROM1 and ROM2 resulted in lethality [3].
  • In addition, overexpression of ROM2 could not rescue cells lacking the regulatory subunit of PKA, indicating that the Ras-adenylate, cyclase-PKA cascade is essential for Rom2p-mediated stress responses and cell growth [4].
  • The effects of the deletion of ROM2 on sensitivity to hydrogen peroxide, paraquat, and cobalt ions, but not to caffeine, were reduced when a constitutive allele of RHO1 was introduced on a single copy plasmid [4].
  • Rom1p and Rom2p are GDP/GTP exchange proteins (GEPs) for the Rho1p small GTP binding protein in Saccharomyces cerevisiae [3].
  • Taken together, our data indicate that Rom2p can regulate PKA activity by controlling cAMP levels via the Ras-cAMP pathway and that for those stresses related to oxidative stress, this cross-talk is probably mediated via the Rho1p-activated MAPK pathway [4].

Anatomical context of ROM2

  • Disruption of ROM2 results in temperature-sensitive growth defects at 11 degrees C and 37 degrees C. rom2 delta cells exhibit morphological defects [5].
  • Furthermore, rom2 delta strains exhibit increased sensitivity to the microtubule depolymerizing drug benomyl [5].
  • Cell wall integrity modulates RHO1 activity via the exchange factor ROM2 [6].

Associations of ROM2 with chemical compounds

  • Subsequent analysis of strains with a rom2 deletion showed that Rom2p is essential for resistance to a variety of stresses caused by freeze-thawing, oxidants, cycloheximide, NaCl, or cobalt ions [4].
  • The mechanism of RHO1 activation by a cell wall alteration is via the exchange factor ROM2 and could be analogous to signalling by integrin receptors in mammalian cells [6].
  • Consistent with this, deletion of the ROM2 gene, which encodes a major Rho1p guanine-nucleotide exchange factor, bypassed the lethal effect of PtdIns(3)P accumulation in ymr1Delta sjl2Delta sjl3Delta triple-mutant cells [7].
  • Yeast phosphatidylinositol 4-phosphate 5-kinases Mss4 and Its3 are involved in resistance against disturbance of sphingolipid biosynthesis and maintenance of cell integrity through the synthesis of PtdIns(4,5)P2 and downstream signalling through the Rom2/Rho2 and Rgf1/Rho pathways [8].

Physical interactions of ROM2


Regulatory relationships of ROM2

  • These results indicate that Rom1p and Rom2p are GEPs that activate Rho1p in S.cerevisiae [3].
  • Stress sensitivity and the growth defect caused by the rom2 deletion could be suppressed by depleting Ras or protein kinase A (PKA) activity or by overexpressing the high affinity cAMP phosphodiesterase Pde2p [4].

Other interactions of ROM2

  • ROM2 was isolated as a suppresser of heat and NaCl sensitivity caused by the lack of the Ras-GTPase activator Ira2p or of cAMP phosphodiesterases [4].
  • A deletion of either ROM2 or WSC1 leads to a significant defect of 1,3-beta-glucan synthesis [10].
  • This result may pose Rom2p as a mediator in the interplay between Tor1p and the Ras/cAMP pathway [11].
  • Indeed, failure of Rom2 recruitment was observed in ISP-1-treated cells as well as in csg2-deleted cells, which have reduced mannosylated inositolphosphorylceramide [12].
  • Pkc1 and the upstream elements of the cell integrity pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, are required for cellular responses to oxidative stress [13].


  1. The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2. Schmidt, A., Bickle, M., Beck, T., Hall, M.N. Cell (1997) [Pubmed]
  2. Lack of GTP-bound Rho1p in secretory vesicles of Saccharomyces cerevisiae. Abe, M., Qadota, H., Hirata, A., Ohya, Y. J. Cell Biol. (2003) [Pubmed]
  3. Rom1p and Rom2p are GDP/GTP exchange proteins (GEPs) for the Rho1p small GTP binding protein in Saccharomyces cerevisiae. Ozaki, K., Tanaka, K., Imamura, H., Hihara, T., Kameyama, T., Nonaka, H., Hirano, H., Matsuura, Y., Takai, Y. EMBO J. (1996) [Pubmed]
  4. Rom2p, the Rho1 GTP/GDP exchange factor of Saccharomyces cerevisiae, can mediate stress responses via the Ras-cAMP pathway. Park, J.I., Collinson, E.J., Grant, C.M., Dawes, I.W. J. Biol. Chem. (2005) [Pubmed]
  5. The Rho-GEF Rom2p localizes to sites of polarized cell growth and participates in cytoskeletal functions in Saccharomyces cerevisiae. Manning, B.D., Padmanabha, R., Snyder, M. Mol. Biol. Cell (1997) [Pubmed]
  6. Cell wall integrity modulates RHO1 activity via the exchange factor ROM2. Bickle, M., Delley, P.A., Schmidt, A., Hall, M.N. EMBO J. (1998) [Pubmed]
  7. PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathway. Parrish, W.R., Stefan, C.J., Emr, S.D. J. Cell. Sci. (2005) [Pubmed]
  8. Supervised membrane swimming: small G-protein lifeguards regulate PIPK signalling and monitor intracellular PtdIns(4,5)P2 pools. Santarius, M., Lee, C.H., Anderson, R.A. Biochem. J. (2006) [Pubmed]
  9. Mutational analysis of the cytoplasmic domain of the Wsc1 cell wall stress sensor. Vay, H.A., Philip, B., Levin, D.E. Microbiology (Reading, Engl.) (2004) [Pubmed]
  10. Dissection of upstream regulatory components of the Rho1p effector, 1,3-beta-glucan synthase, in Saccharomyces cerevisiae. Sekiya-Kawasaki, M., Abe, M., Saka, A., Watanabe, D., Kono, K., Minemura-Asakawa, M., Ishihara, S., Watanabe, T., Ohya, Y. Genetics (2002) [Pubmed]
  11. Investigating the caffeine effects in the yeast Saccharomyces cerevisiae brings new insights into the connection between TOR, PKC and Ras/cAMP signalling pathways. Kuranda, K., Leberre, V., Sokol, S., Palamarczyk, G., François, J. Mol. Microbiol. (2006) [Pubmed]
  12. Disturbance of sphingolipid biosynthesis abrogates the signaling of Mss4, phosphatidylinositol-4-phosphate 5-kinase, in yeast. Kobayashi, T., Takematsu, H., Yamaji, T., Hiramoto, S., Kozutsumi, Y. J. Biol. Chem. (2005) [Pubmed]
  13. Pkc1 and the upstream elements of the cell integrity pathway in Saccharomyces cerevisiae, Rom2 and Mtl1, are required for cellular responses to oxidative stress. Vilella, F., Herrero, E., Torres, J., de la Torre-Ruiz, M.A. J. Biol. Chem. (2005) [Pubmed]
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