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Gene Review

PMT2  -  dolichyl-phosphate-mannose-protein...

Saccharomyces cerevisiae S288c

Synonyms: Dolichyl-phosphate-mannose--protein mannosyltransferase 2, FUN25, YAL023C
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High impact information on PMT2

  • These phenotypes are partially suppressed by overexpression of upstream elements of the protein kinase C (PKC1) cell integrity pathway, suggesting that the PKC1 pathway is defective in pmt2 pmt4Delta mutants [1].
  • Congruently, induction of Mpk1p/Slt2p tyrosine phosphorylation does not occur in pmt2 pmt4Delta mutants during exposure to mating pheromone or elevated temperature [1].
  • We found that pmt2 pmt4Delta cells lyse as small-budded cells in the absence of osmotic stabilization and that treatment with mating pheromone causes pheromone-induced cell death [1].
  • We demonstrate that Mid2 is not mannosylated in a pmt2 mutant and that this modification is important for signaling by Mid2 [2].
  • In a related line of investigation, we identified the PMT2 gene in a genetic screen for mutations that confer an additive cell lysis defect with a wsc1 null allele [2].

Biological context of PMT2

  • The triple disruption pmt2 pmt3 pmt4 and the quadruple disruption result in a lethal phenotype [3].
  • The PMT2 gene from Saccharomyces cerevisiae was identified as FUN25, a transcribed open reading frame on the left arm of chromosome I (Ouellette, B. F. F., Clark, M. W. C., Keng, T., Storms, R. G., Zhong, W., Zeng, B., Fortin, N., Delaney, S., Barton, A., Kaback, D.B., and Bussey, H. (1993) Genome 36, 32-42) [4].
  • We found that the lack of Pmt1 or Pmt2 protein O-mannosyltransferase activity limited the glycosylation of CBHII, but it did not affect its secretion [5].

Anatomical context of PMT2

  • We found that a fraction of mutant alpha-factor precursor, but not the wild type, was progressively O-mannosylated in microsomes and in intact yeast cells by protein O-mannosyl transferase 2 (Pmt2p) [6].
  • Using covalently linked cell-wall protein 5 (Ccw5) as a model, we show that the Pmt4 and Pmt1/Pmt2 mannosyltransferases glycosylate different domains of the Ccw5 protein, thereby mannosylating several consecutive serine and threonine residues [7].
  • The protein O-mannosyltransferases Pmt1p and Pmt2p are catalyzing the O-glycosylation of serine and threonine residues in the endoplasmic reticulum of yeast [8].

Associations of PMT2 with chemical compounds


Physical interactions of PMT2

  • N-glycosylation is initiated by the oligosaccharyl transferase complex and O-mannosylation is initiated by distinct O-mannosyltransferase complexes of the protein mannosyl transferase Pmt1/Pmt2 and Pmt4 families [7].

Other interactions of PMT2

  • Protein O-glycosylation in yeast. The PMT2 gene specifies a second protein O-mannosyltransferase that functions in addition to the PMT1-encoded activity [4].
  • Phylogenetic analysis indicates that PmtA is most closely related to the S. cerevisiae sub-family of PMTs containing Pmt2, Pmt3 and Pmt6 [9].


  1. Aberrant processing of the WSC family and Mid2p cell surface sensors results in cell death of Saccharomyces cerevisiae O-mannosylation mutants. Lommel, M., Bagnat, M., Strahl, S. Mol. Cell. Biol. (2004) [Pubmed]
  2. Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling that act through Rom2, a guanine nucleotide exchange factor for Rho1. Philip, B., Levin, D.E. Mol. Cell. Biol. (2001) [Pubmed]
  3. PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae. Immervoll, T., Gentzsch, M., Tanner, W. Yeast (1995) [Pubmed]
  4. Protein O-glycosylation in yeast. The PMT2 gene specifies a second protein O-mannosyltransferase that functions in addition to the PMT1-encoded activity. Lussier, M., Gentzsch, M., Sdicu, A.M., Bussey, H., Tanner, W. J. Biol. Chem. (1995) [Pubmed]
  5. Protein glycosylation in pmt mutants of Saccharomyces cerevisiae. Influence of heterologously expressed cellobiohydrolase II of Trichoderma reesei and elevated levels of GDP-mannose and cis-prenyltransferase activity. Górka-Nieć, W., Bańkowska, R., Palamarczyk, G., Krotkiewski, H., Kruszewska, J.S. Biochim. Biophys. Acta (2007) [Pubmed]
  6. O-mannosylation protects mutant alpha-factor precursor from endoplasmic reticulum-associated degradation. Harty, C., Strahl, S., Römisch, K. Mol. Biol. Cell (2001) [Pubmed]
  7. O-mannosylation precedes and potentially controls the N-glycosylation of a yeast cell wall glycoprotein. Ecker, M., Mrsa, V., Hagen, I., Deutzmann, R., Strahl, S., Tanner, W. EMBO Rep. (2003) [Pubmed]
  8. Protein O-glycosylation in Saccharomyces cerevisiae: the protein O-mannosyltransferases Pmt1p and Pmt2p function as heterodimer. Gentzsch, M., Immervoll, T., Tanner, W. FEBS Lett. (1995) [Pubmed]
  9. Aspergillus nidulans polarity mutant swoA is complemented by protein O-mannosyltransferase pmtA. Shaw, B.D., Momany, M. Fungal Genet. Biol. (2002) [Pubmed]
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