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Gene Review

PMT1  -  Pmt1p

Saccharomyces cerevisiae S288c

Synonyms: D2390, Dolichyl-phosphate-mannose--protein mannosyltransferase 1, YDL095W
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High impact information on PMT1


Biological context of PMT1

  • A search of the Schizosaccharomyces pombe genome database revealed a total of three O-glycoside mannosyltransferase homologs (ogm1+, ogm2+, and ogm4+), closely related to Saccharomyces cerevisiae PMT1, PMT2, and PMT4 [5].
  • Aga1p and the alpha-agglutinin Agalpha1p were shown to be under glycosylated in cells lacking the protein mannosyltransferase genes PMT1 and PMT2, with phenotypes manifested only in MATalpha cells for single mutants but in both cell types when both genes are absent [6].
  • In addition, multiple specific deficiencies not known in Saccharomyces cerevisiae (including defective hyphal morphogenesis; supersensitivity to the antifungal agents hygromycin B, G418, clotrimazole, and calcofluor white; and reduced adherence to Caco-2 epithelial cells) were observed in pmt1 mutants [4].
  • We found that the lack of Pmt1 or Pmt2 protein O-mannosyltransferase activity limited the glycosylation of CBHII, but it did not affect its secretion [7].

Anatomical context of PMT1

  • Pmt1 mannosyl transferase is involved in cell wall incorporation of several proteins in Saccharomyces cerevisiae [8].
  • However, the plasma membrane protein Gas1p still receives its glycosyl-phosphatidylinositol anchor in pmt1 cells, and in this mutant strain an alpha-galactosidase-Cwp2 fusion protein was found linked to the cell wall but devoid of beta1,6-glucan side-chain, indicating an alternative mechanism of cell wall anchorage [8].
  • In Saccharomyces cerevisiae, O-mannosylation is initiated in the lumen of the endoplasmic reticulum by O-mannosyltransferase gene products (Pmt1p-7p) [5].

Associations of PMT1 with chemical compounds

  • Pmt1p and Pmt2p are mannosyltransferases involved in the transfer of a mannosyl residue from dolichyl phosphate-D-mannose (Dol-P-Man) to seryl and threonyl residues in proteins [9].
  • These results also demonstrate that maximal O-mannosylation of serine/threonine residues in yeast glycoproteins catalyzed by the partially purified preparation of PMT1 requires the presence of a saturated alpha-isoprene unit in the dolichyl moiety of Man-P-Dol [10].
  • These very stable, partially purified preparations of PMT1 catalyzed the transfer of mannosyl units from exogenous Man-P-Dol to serine/threonine residues in the synthetic peptide acceptor, Tyr-Asn-Pro-Thr-Ser-Val-NH2, forming O-mannosidic linkages of the alpha-configuration [10].
  • The chain length of the dolichyl moiety also influenced the mannolipid-enzyme interaction as the partially purified PMT1 had a higher affinity for Man-P-Dol95 than for Man-P-Dol55 [10].
  • Mutants lacking both PMT1 and PMT6 are viable and show pmt1 mutant phenotypes and an additional sensitivity to the iron chelator ethylenediamine-di(o-hydroxyphenylacetic acid) [11].

Physical interactions of PMT1

  • N-glycosylation is initiated by the oligosaccharyl transferase complex and O-mannosylation is initiated by distinct O-mannosyltransferase complexes of the protein mannosyl transferase Pmt1/Pmt2 and Pmt4 families [12].

Regulatory relationships of PMT1

  • The results suggest that Pmt6p regulates a more narrow subclass of proteins in C. albicans than Pmt1p, including secreted proteins responsible for morphogenesis and antifungal sensitivities [11].

Other interactions of PMT1

  • Protein O-glycosylation in yeast. The PMT2 gene specifies a second protein O-mannosyltransferase that functions in addition to the PMT1-encoded activity [13].
  • We found that a pmt1 deletion mutant was highly sensitive to zymolyase and that in this strain the alpha-galactosidase-Srp1 fusion proteins, an alpha-galactosidase-Sed1 hybrid protein and an alpha-galactosidase-alpha-agglutinin hybrid protein were absent from both the membrane and the cell wall fractions [8].
  • The glaA gene encoding glucoamylase I (GAI) of Aspergillus awamori var. kawachi was heterologously expressed in mannosyltransferase mutants of Saccharomyces cerevisiae, in which the pmt1 gene and the kre2 gene were disrupted [14].


  1. Members of the evolutionarily conserved PMT family of protein O-mannosyltransferases form distinct protein complexes among themselves. Girrbach, V., Strahl, S. J. Biol. Chem. (2003) [Pubmed]
  2. Structure-function analysis of the dolichyl phosphate-mannose: protein O-mannosyltransferase ScPmt1p. Girrbach, V., Zeller, T., Priesmeier, M., Strahl-Bolsinger, S. J. Biol. Chem. (2000) [Pubmed]
  3. Transmembrane topology of pmt1p, a member of an evolutionarily conserved family of protein O-mannosyltransferases. Strahl-Bolsinger, S., Scheinost, A. J. Biol. Chem. (1999) [Pubmed]
  4. Multiple functions of Pmt1p-mediated protein O-mannosylation in the fungal pathogen Candida albicans. Timpel, C., Strahl-Bolsinger, S., Ziegelbauer, K., Ernst, J.F. J. Biol. Chem. (1998) [Pubmed]
  5. Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe. Tanaka, N., Fujita, Y., Suzuki, S., Morishita, M., Giga-Hama, Y., Shimoda, C., Takegawa, K. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
  6. Posttranslational modifications required for cell surface localization and function of the fungal adhesin Aga1p. Huang, G., Zhang, M., Erdman, S.E. Eukaryotic Cell (2003) [Pubmed]
  7. Protein glycosylation in pmt mutants of Saccharomyces cerevisiae. Influence of heterologously expressed cellobiohydrolase II of Trichoderma reesei and elevated levels of GDP-mannose and cis-prenyltransferase activity. Górka-Nieć, W., Bańkowska, R., Palamarczyk, G., Krotkiewski, H., Kruszewska, J.S. Biochim. Biophys. Acta (2007) [Pubmed]
  8. Pmt1 mannosyl transferase is involved in cell wall incorporation of several proteins in Saccharomyces cerevisiae. Bourdineaud, J.P., van der Vaart, J.M., Donzeau, M., de Sampaïo, G., Verrips, C.T., Lauquin, G.J. Mol. Microbiol. (1998) [Pubmed]
  9. PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae. Immervoll, T., Gentzsch, M., Tanner, W. Yeast (1995) [Pubmed]
  10. Mannosylphosphoryldolichol-mediated O-mannosylation of yeast glycoproteins: stereospecificity and recognition of the alpha-isoprene unit by a purified mannosyltransferase. Dotson, S.B., Rush, J.S., Ricketts, A.D., Waechter, C.J. Arch. Biochem. Biophys. (1995) [Pubmed]
  11. Morphogenesis, adhesive properties, and antifungal resistance depend on the Pmt6 protein mannosyltransferase in the fungal pathogen candida albicans. Timpel, C., Zink, S., Strahl-Bolsinger, S., Schröppel, K., Ernst, J. J. Bacteriol. (2000) [Pubmed]
  12. O-mannosylation precedes and potentially controls the N-glycosylation of a yeast cell wall glycoprotein. Ecker, M., Mrsa, V., Hagen, I., Deutzmann, R., Strahl, S., Tanner, W. EMBO Rep. (2003) [Pubmed]
  13. Protein O-glycosylation in yeast. The PMT2 gene specifies a second protein O-mannosyltransferase that functions in addition to the PMT1-encoded activity. Lussier, M., Gentzsch, M., Sdicu, A.M., Bussey, H., Tanner, W. J. Biol. Chem. (1995) [Pubmed]
  14. Functional analysis of O-linked oligosaccharides in threonine/serine-rich region of Aspergillus glucoamylase by expression in mannosyltransferase-disruptants of yeast. Goto, M., Tsukamoto, M., Kwon, I., Ekino, K., Furukawa, K. Eur. J. Biochem. (1999) [Pubmed]
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