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RFA1  -  Rfa1p

Saccharomyces cerevisiae S288c

Synonyms: BUF2, DNA-binding protein BUF2, FUN3, RF-A protein 1, RPA1, ...
 
 
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High impact information on RFA1

 

Biological context of RFA1

  • RFA1 regulates DNA repair genes at the transcriptional level [6].
  • Downregulation of RFA1 in rho(0) cells is consistent with our finding that mitochondrial dysfunction leads to instability of the nuclear genome [6].
  • The RFA1 gene encodes the large subunit of the yeast trimeric single-stranded DNA binding protein replication protein A (RPA), which is known to play a critical role in DNA replication [7].
  • Presented is a model accounting for this genetic interaction in which Rfa1, in a complex with Rad52, serves to assemble other proteins of the recombination-repair machinery at the site of DSBs and other kinds of DNA damage [8].
  • An allele of RFA1, the largest subunit of the single-stranded DNA-binding complex RP-A, was identified as a suppressor of decreased direct-repeat recombination in rad1 rad52 double mutants [9].
 

Associations of RFA1 with chemical compounds

 

Physical interactions of RFA1

 

Regulatory relationships of RFA1

 

Other interactions of RFA1

  • Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA [7].
  • In addition, we identified physical and functional interactions between these proteins and found that RPA binds Dna2 predominantly through its large subunit, Rpa1 [19].
  • A mutation in RFA1, the largest subunit of a single-stranded DNA-binding protein complex (RP-A), suppresses the recombination deficiency of rad1 rad52 strains (J. Smith and R. Rothstein, Mol. Cell. Biol. 15:1632-1641, 1995) [20].
  • We have previously shown that replication- protein A (RPA), the heterotrimeric single-stranded DNA binding protein of eukaryotes, plays a role in Okazaki fragment processing by acting as a molecular switch between the two endonucleases, Dna2 and Fen1, to ensure the complete removal of primer RNAs in Saccharomyces cerevisiae [19].
  • To identify proteins interacting with the Mec1-Ddc2 complex, we performed a modified two-hybrid screen and isolated RFA1 and RFA2, genes that encode subunits of replication protein A (RPA) [21].

References

  1. RPA regulates telomerase action by providing Est1p access to chromosome ends. Schramke, V., Luciano, P., Brevet, V., Guillot, S., Corda, Y., Longhese, M.P., Gilson, E., Géli, V. Nat. Genet. (2004) [Pubmed]
  2. Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins. Lisby, M., Barlow, J.H., Burgess, R.C., Rothstein, R. Cell (2004) [Pubmed]
  3. Telomere elongation by hnRNP A1 and a derivative that interacts with telomeric repeats and telomerase. LaBranche, H., Dupuis, S., Ben-David, Y., Bani, M.R., Wellinger, R.J., Chabot, B. Nat. Genet. (1998) [Pubmed]
  4. Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase. Brill, S.J., Stillman, B. Genes Dev. (1991) [Pubmed]
  5. Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells. Din, S., Brill, S.J., Fairman, M.P., Stillman, B. Genes Dev. (1990) [Pubmed]
  6. Genome-wide analysis of signal transducers and regulators of mitochondrial dysfunction in Saccharomyces cerevisiae. Singh, K.K., Rasmussen, A.K., Rasmussen, L.J. Ann. N. Y. Acad. Sci. (2004) [Pubmed]
  7. Studies of the interaction between Rad52 protein and the yeast single-stranded DNA binding protein RPA. Hays, S.L., Firmenich, A.A., Massey, P., Banerjee, R., Berg, P. Mol. Cell. Biol. (1998) [Pubmed]
  8. A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52. Firmenich, A.A., Elias-Arnanz, M., Berg, P. Mol. Cell. Biol. (1995) [Pubmed]
  9. An allele of RFA1 suppresses RAD52-dependent double-strand break repair in Saccharomyces cerevisiae. Smith, J., Rothstein, R. Genetics (1999) [Pubmed]
  10. A mutation in the gene encoding the Saccharomyces cerevisiae single-stranded DNA-binding protein Rfa1 stimulates a RAD52-independent pathway for direct-repeat recombination. Smith, J., Rothstein, R. Mol. Cell. Biol. (1995) [Pubmed]
  11. ATP utilization by yeast replication factor C. I. ATP-mediated interaction with DNA and with proliferating cell nuclear antigen. Gomes, X.V., Burgers, P.M. J. Biol. Chem. (2001) [Pubmed]
  12. Phosphorylation of the replication protein A large subunit in the Saccharomyces cerevisiae checkpoint response. Brush, G.S., Kelly, T.J. Nucleic Acids Res. (2000) [Pubmed]
  13. Rfc4 interacts with Rpa1 and is required for both DNA replication and DNA damage checkpoints in Saccharomyces cerevisiae. Kim, H.S., Brill, S.J. Mol. Cell. Biol. (2001) [Pubmed]
  14. A single-stranded DNA-binding protein is needed for efficient presynaptic complex formation by the Saccharomyces cerevisiae Rad51 protein. Sugiyama, T., Zaitseva, E.M., Kowalczykowski, S.C. J. Biol. Chem. (1997) [Pubmed]
  15. CTBP1/RBP1, a Saccharomyces cerevisiae protein which binds to T-rich single-stranded DNA containing the 11-bp core sequence of autonomously replicating sequence, is a poly(deoxypyrimidine)-binding protein. Ikeda, M., Arai, K., Masai, H. Eur. J. Biochem. (1996) [Pubmed]
  16. Saccharomyces cerevisiae proteins involved in hybrid DNA formation in vitro. Heyer, W.D., Johnson, A.W., Norris, D.N., Tishkoff, D., Kolodner, R.D. Biochimie (1991) [Pubmed]
  17. Isolation, DNA sequence, and regulation of a Saccharomyces cerevisiae gene that encodes DNA strand transfer protein alpha. Clark, A.B., Dykstra, C.C., Sugino, A. Mol. Cell. Biol. (1991) [Pubmed]
  18. A Saccharomyces cerevisiae DNA helicase associated with replication factor C. Li, X., Yoder, B.L., Burgers, P.M. J. Biol. Chem. (1992) [Pubmed]
  19. Bimodal interaction between replication-protein A and Dna2 is critical for Dna2 function both in vivo and in vitro. Bae, K.H., Kim, H.S., Bae, S.H., Kang, H.Y., Brill, S., Seo, Y.S. Nucleic Acids Res. (2003) [Pubmed]
  20. Rsp5, a ubiquitin-protein ligase, is involved in degradation of the single-stranded-DNA binding protein rfa1 in Saccharomyces cerevisiae. Erdeniz, N., Rothstein, R. Mol. Cell. Biol. (2000) [Pubmed]
  21. Role of the C terminus of Mec1 checkpoint kinase in its localization to sites of DNA damage. Nakada, D., Hirano, Y., Tanaka, Y., Sugimoto, K. Mol. Biol. Cell (2005) [Pubmed]
 
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