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Gene Review

UGA4  -  Uga4p

Saccharomyces cerevisiae S288c

Synonyms: D1037, GABA-specific permease, GABA-specific transport protein, YDL210W
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High impact information on UGA4

  • The predicted protein contains 517 residues and shows 30.3% overall identity with a putative GABA permease of Arabidopsis thaliana, 29.6% identity with the yeast choline transporter and 23.4% identity with the yeast UGA4 GABA permease [1].
  • The inducible UGA4 gene, which encodes the specific gamma-aminobutyrate permease, undergoes strong repression by Uga43p [2].
  • The role of Gln3p is less predominant in UGA1 than in UGA4 gene expression [3].
  • Further analysis of the UGA4 gene revealed that Gln3p, a global nitrogen regulatory protein containing a GATA zinc-finger domain, is required in order to reach high levels of gamma-aminobutyrate-induced transcription [3].
  • Northern blotting of the nontransformed and the M-gamma-A-transformed 22574d cells with gene-specific probes for the three proline transporters showed the expression of an mRNA for UGA4 in both transformed and nontransformed cells but no evidence for the expression of GAP1 or PUT4 [4].

Biological context of UGA4

  • Here we show that DAL80 protein binds to DAL3 and UGA4 upstream DNA sequences, designated URSGATA, consisting of two GATAA-containing sites separated by at least 15 bp [5].
  • (a) Recessive mutations in the UGA43 gene suggest that the product of the UGA43 gene behaves like a trans-acting negative regulator of UGA4 gene expression [6].
  • The predicted amino acid sequence shows that UGA4 encodes a 62 kDa protein having 9-12 putative membrane-spanning regions [7].
  • METHODS: we analyzed the effect of pH on the expression of UGA4 gene measuring beta-galactosidase activity in cells carrying a UGA4::lacZ fusion gene [8].
  • Analysis of the 5' region of the UGA1 and UGA4 genes led to the identification of a conserved GC-rich sequence (UASGABA) essential to induction by gamma-aminobutyrate [3].

Anatomical context of UGA4


Associations of UGA4 with chemical compounds


Other interactions of UGA4

  • In the absence of the pleiotropic UGA43/DAL80 repressor, UGA4 is constitutively expressed at high level [7].
  • In Saccharomyces cerevisiae, the pathway of 4-aminobutyric acid catabolism, for use as a nitrogen source, involves a specific permease (encoded by the UGA4 gene) and two enzymes (encoded by the UGA1 and UGA2 genes, respectively) [6].
  • Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p [11].
  • This regulation is probably mediated by Rim101p through the consensus site 5'-GCCARG-3' at 237 bp preceding the UGA4 coding sequence (201) [8].

Analytical, diagnostic and therapeutic context of UGA4


  1. The multiply-regulated gabA gene encoding the GABA permease of Aspergillus nidulans: a score of exons. Hutchings, H., Stahmann, K.P., Roels, S., Espeso, E.A., Timberlake, W.E., Arst, H.N., Tilburn, J. Mol. Microbiol. (1999) [Pubmed]
  2. Two mutually exclusive regulatory systems inhibit UASGATA, a cluster of 5'-GAT(A/T)A-3' upstream from the UGA4 gene of Saccharomyces cerevisiae. André, B., Talibi, D., Soussi Boudekou, S., Hein, C., Vissers, S., Coornaert, D. Nucleic Acids Res. (1995) [Pubmed]
  3. Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae. Talibi, D., Grenson, M., André, B. Nucleic Acids Res. (1995) [Pubmed]
  4. Mutated-gamma-actin restores growth to a yeast amino acid transport defective mutant. Khamessan, A., Naghibalhossaini, F., Vedadi, M., Johnstone, R.M. J. Cell. Physiol. (2001) [Pubmed]
  5. The Saccharomyces cerevisiae DAL80 repressor protein binds to multiple copies of GATAA-containing sequences (URSGATA). Cunningham, T.S., Cooper, T.G. J. Bacteriol. (1993) [Pubmed]
  6. Positive and negative regulatory elements control the expression of the UGA4 gene coding for the inducible 4-aminobutyric-acid-specific permease in Saccharomyces cerevisiae. Vissers, S., Andre, B., Muyldermans, F., Grenson, M. Eur. J. Biochem. (1989) [Pubmed]
  7. Cloning and expression of the UGA4 gene coding for the inducible GABA-specific transport protein of Saccharomyces cerevisiae. André, B., Hein, C., Grenson, M., Jauniaux, J.C. Mol. Gen. Genet. (1993) [Pubmed]
  8. UGA4 gene encoding the gamma-aminobutyric acid permease in Saccharomyces cerevisiae is an acid-expressed gene. Moretti, M.B., Batlle, A., Garcia, S.C. Int. J. Biochem. Cell Biol. (2001) [Pubmed]
  9. Uptake of GABA and putrescine by UGA4 on the vacuolar membrane in Saccharomyces cerevisiae. Uemura, T., Tomonari, Y., Kashiwagi, K., Igarashi, K. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
  10. Carbon and nitrogen sources regulate delta-aminolevulinic acid and gamma-aminobutyric acid transport in Saccharomyces cerevisiae. Correa García, S., Bermúdez Moretti, M., Ramos, E., Batlle, A. Int. J. Biochem. Cell Biol. (1997) [Pubmed]
  11. Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p. Garcia, S.C., Moretti, M.B., Batlle, A. FEMS Microbiol. Lett. (2000) [Pubmed]
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