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Gene Review

STE7  -  Ste7p

Saccharomyces cerevisiae S288c

Synonyms: D1525, Serine/threonine-protein kinase STE7, YDL159W
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Disease relevance of STE7

  • The presence of the interacting N1-Fus3p adapter increased the infectivity of Ste7p-N2-CT phages approximately 1400-fold, which makes SIP a promising technology for the detection and further investigation of interacting proteins [1].

High impact information on STE7

  • Overproduction decreases when haploid ROAM mutants also contain the ste7 mutation which prevents conjugation; other ste mutations do not affect the expression of ROAM mutations [2].
  • Kss1 and Fus3, the MAPK targets of Ste7, are required for mating, but their role in invasive growth has been unclear [3].
  • We show instead that Kss1 is the principal target of Ste7 in the invasive-growth response in both haploids and diploids [3].
  • FUS3/KSS1 phosphorylation depends on two additional kinases, STE11 and STE7 (refs 2, 5, 6) [4].
  • Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK [5].

Biological context of STE7

  • Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase [6].
  • The signal transduction pathway that mediates the response of haploid yeast cells to peptide mating pheromones involves several components including the protein kinases STE7 and STE11 [6].
  • Cell viability was not restored by a mutation in STE7 that blocks signaling downstream of the G protein [7].
  • CaSTE7 behaves as a hyperactive allele of STE7, suppressing the mating defects of the pheromone signal-transduction pathway by constitutively stimulating STE12, and hence STE12-dependent processes [8].
  • Partial protein sequencing of purified activator indicates that it contains a sequence homologous to kinase subdomains VI and VII of two yeast protein kinases, STE7 and byrl [9].

Anatomical context of STE7

  • Sph1p interacts in the two-hybrid system with three mitogen-activated protein (MAP) kinase kinases (MAPKKs): Mkk1p and Mkk2p, which function in the cell wall integrity/cell polarization MAP kinase pathway, and Ste7p, which operates in the pheromone and pseudohyphal signaling response pathways [10].
  • We now report that stimulation with serum of COS cells expressing this shares sequence homology with two yeast protein kinases byr1 and STE7 [11].

Associations of STE7 with chemical compounds


Physical interactions of STE7

  • We also found that both Ste11p and Ste7p interact with Fus3p and Kss1p [14].
  • Differences in the ability of constitutive Ste7 variants to bind the MAPKs and Ste5 account for the selective activation of Kss1 [15].

Enzymatic interactions of STE7

  • FUS3 and presumably KSS1 are phosphorylated and activated during pheromone induction by a STE7-dependent mechanism [16].

Regulatory relationships of STE7

  • MAP kinase-related FUS3 from S. cerevisiae is activated by STE7 in vitro [4].
  • Byr1 and Ste7 are in turn regulated by the protein kinases Byr2 and Ste11 [17].
  • Upon pheromone stimulation, ubp3 Delta mutants accumulate unconjugated polyubiquitin chains as well as polyubiquitinated forms of the mitogen-activated protein kinase kinase Ste7 [18].

Other interactions of STE7

  • In addition, the activity of the MAT alpha 1-LexA fusion protein is dependent on the functions of the STE7, STE11, and STE12 genes that encode components of the pheromone response pathway [19].
  • Genetic analysis indicates that MSG5 acts at a stage where the protein kinases STE7 and FUS3 function to transmit the pheromone-induced signal [20].
  • Neither protein altered the ability of the mitogen-activated protein kinase (MAPK) Fus3 to feedback phosphorylate a known substrate, the MAPK kinase Ste7 [21].
  • Additionally, the cellular projections formed by a pbs2-3 mutant on high osmolarity are absent in strains lacking KSS1 or STE7 [22].
  • The ste7, 11, and 12 mutations were not suppressed by ros1 or sst2 [23].

Analytical, diagnostic and therapeutic context of STE7

  • We report here the molecular cloning of STE7 and STE11 genes and show that expression of these genes is not regulated transcriptionally by the MAT locus [24].


  1. In vivo selectively infective phage as a tool to detect protein interactions: evaluation of a novel vector system with yeast Ste7p-Fus3p interacting proteins. Hertveldt, K., Robben, J., Volckaert, G. Yeast (2002) [Pubmed]
  2. Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes. Errede, B., Cardillo, T.S., Sherman, F., Dubois, E., Deschamps, J., Wiame, J.M. Cell (1980) [Pubmed]
  3. Inhibitory and activating functions for MAPK Kss1 in the S. cerevisiae filamentous-growth signalling pathway. Cook, J.G., Bardwell, L., Thorner, J. Nature (1997) [Pubmed]
  4. MAP kinase-related FUS3 from S. cerevisiae is activated by STE7 in vitro. Errede, B., Gartner, A., Zhou, Z., Nasmyth, K., Ammerer, G. Nature (1993) [Pubmed]
  5. Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK. Bardwell, L., Cook, J.G., Voora, D., Baggott, D.M., Martinez, A.R., Thorner, J. Genes Dev. (1998) [Pubmed]
  6. Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase. Cairns, B.R., Ramer, S.W., Kornberg, R.D. Genes Dev. (1992) [Pubmed]
  7. Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast. Flanary, P.L., DiBello, P.R., Estrada, P., Dohlman, H.G. J. Biol. Chem. (2000) [Pubmed]
  8. A novel MAP-kinase kinase from Candida albicans. Singh, P., Ghosh, S., Datta, A. Gene (1997) [Pubmed]
  9. Xenopus MAP kinase activator is a serine/threonine/tyrosine kinase activated by threonine phosphorylation. Kosako, H., Gotoh, Y., Matsuda, S., Ishikawa, M., Nishida, E. EMBO J. (1992) [Pubmed]
  10. The Spa2-related protein, Sph1p, is important for polarized growth in yeast. Roemer, T., Vallier, L., Sheu, Y.J., Snyder, M. J. Cell. Sci. (1998) [Pubmed]
  11. Functional expression of a MAP kinase kinase in COS cells and recognition by an anti-STE7/byr1 antibody. Haystead, C.M., Wu, J., Gregory, P., Sturgill, T.W., Haystead, T.A. FEBS Lett. (1993) [Pubmed]
  12. Dynamics and organization of MAP kinase signal pathways. Errede, B., Cade, R.M., Yashar, B.M., Kamada, Y., Levin, D.E., Irie, K., Matsumoto, K. Mol. Reprod. Dev. (1995) [Pubmed]
  13. Cloning and characterization of a dual-specificity kinase gene in rice (Oryza sative). Du, H., Liang, Y. Yi Chuan Xue Bao (2005) [Pubmed]
  14. Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Printen, J.A., Sprague, G.F. Genetics (1994) [Pubmed]
  15. Persistent activation by constitutive Ste7 promotes Kss1-mediated invasive growth but fails to support Fus3-dependent mating in yeast. Maleri, S., Ge, Q., Hackett, E.A., Wang, Y., Dohlman, H.G., Errede, B. Mol. Cell. Biol. (2004) [Pubmed]
  16. Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases. Zhou, Z., Gartner, A., Cade, R., Ammerer, G., Errede, B. Mol. Cell. Biol. (1993) [Pubmed]
  17. A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf. Lange-Carter, C.A., Pleiman, C.M., Gardner, A.M., Blumer, K.J., Johnson, G.L. Science (1993) [Pubmed]
  18. Pheromone-dependent ubiquitination of the mitogen-activated protein kinase kinase Ste7. Wang, Y., Dohlman, H.G. J. Biol. Chem. (2002) [Pubmed]
  19. MAT alpha 1 can mediate gene activation by a-mating factor. Sengupta, P., Cochran, B.H. Genes Dev. (1991) [Pubmed]
  20. MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae. Doi, K., Gartner, A., Ammerer, G., Errede, B., Shinkawa, H., Sugimoto, K., Matsumoto, K. EMBO J. (1994) [Pubmed]
  21. Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast. Burchett, S.A., Scott, A., Errede, B., Dohlman, H.G. J. Biol. Chem. (2001) [Pubmed]
  22. Activation of the Saccharomyces cerevisiae filamentation/invasion pathway by osmotic stress in high-osmolarity glycogen pathway mutants. Davenport, K.D., Williams, K.E., Ullmann, B.D., Gustin, M.C. Genetics (1999) [Pubmed]
  23. Saccharomyces cerevisiae mutants unresponsive to alpha-factor pheromone: alpha-factor binding and extragenic suppression. Jenness, D.D., Goldman, B.S., Hartwell, L.H. Mol. Cell. Biol. (1987) [Pubmed]
  24. Molecular cloning and characterization of the STE7 and STE11 genes of Saccharomyces cerevisiae. Chaleff, D.T., Tatchell, K. Mol. Cell. Biol. (1985) [Pubmed]
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