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Gene Review

RAD59  -  Rad59p

Saccharomyces cerevisiae S288c

Synonyms: DNA repair protein RAD59, YDL059C
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High impact information on RAD59

  • Overexpression of RAD52 was found to suppress the DNA repair and recombination defects conferred by the rad59 mutation, suggesting that these proteins have overlapping roles or function as a complex [1].
  • One mutation identified, rad59, reduced recombination 1200-fold in the presence of a rad51 mutation, but only 4- to 5-fold in a wild-type background [1].
  • The rad59 mutant strain was sensitive to ionizing radiation, and this phenotype was used to clone the RAD59 gene by complementation [1].
  • However, the rate of interchromosomal recombination was increased in a rad59 homozygous diploid [1].
  • We found that Rad59p plays a role in removing nonhomologous sequences from the ends of single-stranded DNA when it invades a homologous DNA template, in a manner similar to that previously seen with srs2 mutants [2].

Biological context of RAD59

  • Such a mechanism of recombination is RAD1-RAD59-dependent and yields gene conversions highly associated with the inversion of the repeat [3].
  • The RAD59 gene product also participates in the RAD1-dependent pathway of recombination between direct repeats [4].
  • A mutant with a deletion of RAD59, a homologue of RAD52, was defective for SSA, especially when the homologous-sequence length was short; however, even with 1.17-kb substrates, SSA was reduced fourfold [2].
  • The differences between the RAD51- and RAD50/RAD59-dependent pathways account for the distinct ways that two different recombination processes maintain yeast telomeres in the absence of telomerase [5].
  • Plasmid gap repair was reduced synergistically in rad51 rad59 double mutants, indicating an important role for RAD59 in RAD51-independent repair [6].

Physical interactions of RAD59

  • The yeast recombinational repair protein Rad59 interacts with Rad52 and stimulates single-strand annealing [7].
  • A novel yeast mutation, rad52-L89F, causes a specific defect in Rad51-independent recombination that correlates with a reduced ability of Rad52-L89F to interact with Rad59 [8].

Other interactions of RAD59

  • In the budding yeast Saccharomyces cerevisiae, the RAD52 gene is essential for all homologous recombination events and its homologue, the RAD59 gene, is important for those that occur independently of RAD51 [9].
  • In addition, the regulation of homeologous recombination in rad51 and rad59 mutants displayed distinct patterns of inhibition by different members of the MMR pathway [10].
  • RAD50 and RAD59, genes required for BIR, are required for X-ray-associated SCE but not for SCE stimulated by HO-induced DSBs [11].

Analytical, diagnostic and therapeutic context of RAD59


  1. A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae. Bai, Y., Symington, L.S. Genes Dev. (1996) [Pubmed]
  2. DNA length dependence of the single-strand annealing pathway and the role of Saccharomyces cerevisiae RAD59 in double-strand break repair. Sugawara, N., Ira, G., Haber, J.E. Mol. Cell. Biol. (2000) [Pubmed]
  3. Yeast spt6-140 mutation, affecting chromatin and transcription, preferentially increases recombination in which Rad51p-mediated strand exchange is dispensable. Malagón, F., Aguilera, A. Genetics (2001) [Pubmed]
  4. Characterization of the role played by the RAD59 gene of Saccharomyces cerevisiae in ectopic recombination. Jablonovich, Z., Liefshitz, B., Steinlauf, R., Kupiec, M. Curr. Genet. (1999) [Pubmed]
  5. Characterization of RAD51-independent break-induced replication that acts preferentially with short homologous sequences. Ira, G., Haber, J.E. Mol. Cell. Biol. (2002) [Pubmed]
  6. RAD51 is required for the repair of plasmid double-stranded DNA gaps from either plasmid or chromosomal templates. Bärtsch, S., Kang, L.E., Symington, L.S. Mol. Cell. Biol. (2000) [Pubmed]
  7. The yeast recombinational repair protein Rad59 interacts with Rad52 and stimulates single-strand annealing. Davis, A.P., Symington, L.S. Genetics (2001) [Pubmed]
  8. A novel yeast mutation, rad52-L89F, causes a specific defect in Rad51-independent recombination that correlates with a reduced ability of Rad52-L89F to interact with Rad59. Cortés-Ledesma, F., Malagón, F., Aguilera, A. Genetics (2004) [Pubmed]
  9. DNA annealing mediated by Rad52 and Rad59 proteins. Wu, Y., Sugiyama, T., Kowalczykowski, S.C. J. Biol. Chem. (2006) [Pubmed]
  10. Role of mismatch repair in the fidelity of RAD51- and RAD59-dependent recombination in Saccharomyces cerevisiae. Spell, R.M., Jinks-Robertson, S. Genetics (2003) [Pubmed]
  11. Multiple recombination pathways for sister chromatid exchange in Saccharomyces cerevisiae: role of RAD1 and the RAD52 epistasis group genes. Dong, Z., Fasullo, M. Nucleic Acids Res. (2003) [Pubmed]
  12. The DNA Binding Preference of RAD52 and RAD59 Proteins: IMPLICATIONS FOR RAD52 AND RAD59 PROTEIN FUNCTION IN HOMOLOGOUS RECOMBINATION. Wu, Y., Siino, J.S., Sugiyama, T., Kowalczykowski, S.C. J. Biol. Chem. (2006) [Pubmed]
  13. Rad52 and Rad59 exhibit both overlapping and distinct functions. Feng, Q., D??ring, L., de Mayolo, A.A., Lettier, G., Lisby, M., Erdeniz, N., Mortensen, U.H., Rothstein, R. DNA Repair (Amst.) (2007) [Pubmed]
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