High impact information on BGL2

• The synergistic behavior of the disruption of SCW4 and SCW10 was partly antagonized by the disruption of BGL2 [1].
• Purification and characterization of the Saccharomyces cerevisiae BGL2 gene product, a cell wall endo-beta-1,3-glucanase [2].
• In each case the BGL2 signal peptide (or its variant) has proven equally productive to the corresponding MF alpha 1 peptide [3].
• Haploid yeast cells contained one copy of the gene (BGL2) [4].
• We isolated two genes for extracellular beta-glucosidase, BGL1 and BGL2, from the genomic library of the yeast Saccharomycopsis fibuligera [5].

Biological context of BGL2

• Phenotype in Candida albicans of a disruption of the BGL2 gene encoding a 1,3-beta-glucosyltransferase [6].
• Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene [7].
• The ability of a 23-amino acid (aa) signal peptide derived from the BGL2 gene to direct a heterologous protein to the secretory pathway of yeast has been compared to that of the MF alpha 1-encoded signal peptide in a series of gene fusions [3].
• Kinetics of beta-1,3 glucan interaction at the donor and acceptor sites of the fungal glucosyltransferase encoded by the BGL2 gene [8].

Anatomical context of BGL2

• Deletion of BGL2 results in an increased chitin level in the cell wall of Saccharomyces cerevisiae [9].

Associations of BGL2 with chemical compounds

• This result is consistent with the observation that the S. cerevisiae transformant carrying BGL1 fermented cellobiose to ethanol but the transformant carrying BGL2 did not [5].
• It was found that the lack of the non-covalently bound wall proteins Scw4p, Scw10p and Bgl2p increases the mortality of Saccharomyces cerevisiae cells grown exponentially under standard laboratory conditions, as assayed by methylene blue staining [10].

Regulatory relationships of BGL2

• This suggests that the PKC1 kinase cascade regulates BGL2 expression negatively and overproduction of the beta-glucanase is partially responsible for the growth defect.(ABSTRACT TRUNCATED AT 250 WORDS)[11]

Other interactions of BGL2

• The chitin level in the cell walls of a strain lacking both BGL2 and CHS3 genes was higher than that in chs3Delta and lower than that in bgl2Delta strains [9].
• Disruption of BGL2 in the hpo2 mutant partially rescued the growth rate defect [11].
• For Bgl2p, endoglucanase/glucanosyltransferase activity has been demonstrated, and Scw11p has been shown to be involved in cell separation [12].
• Bgl2p and Gas1p are the major glucan transferases forming the molecular ensemble of yeast cell wall [13].

Analytical, diagnostic and therapeutic context of BGL2

• Strain CACW-1 contained disruptions of both homologues of the BGL2 gene and lacked Bgl2p, as assessed by protein extraction, SDS-PAGE and Western blot analysis, and enzyme assay; however, residual non-Bgl2p transferase activity was detected [6].
• The 35 kDa putative surface protein was identified by N-terminal sequencing and immunoblotting, as the 1,3-beta glucosyltransferase, Bgl2p [14].

References