The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

NDC1  -  Ndc1p

Saccharomyces cerevisiae S288c

Synonyms: Nuclear division cycle protein 1, Nuclear pore protein NDC1, Nucleoporin NDC1, YML031W
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on NDC1

  • We describe the phenotypes caused by a cold-sensitive lethal mutation (ndc1-1) that defines the NDC1 gene of yeast [1].
  • Meiosis II, but not meiosis I, is sensitive to the ndc1-1 defect, suggesting that NDC1 is required for some feature common to mitosis and meiosis II. ndc1-1 appears to define a new class of cell cycle gene required for the attachment of chromosomes to the spindle pole [1].
  • We show by RNA interference (RNAi) and biochemical depletion that NDC1 plays an important role in NPC and NE assembly in vivo and in vitro [2].
  • The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells [2].
  • However, the role of Ndc1p in NPC assembly is partially redundant with Pom152p, as cells lacking both of these proteins show enhanced NPC disruption [3].

Biological context of NDC1


Anatomical context of NDC1

  • We report a novel connection between nuclear pore complexes (NPCs) and spindle pole bodies (SPBs) revealed by our studies of the Saccharomyces cerevisiae NDC1 gene [7].
  • Mutation of either the yeast MPS2 or the NDC1 gene leads to identical spindle pole body (SPB) duplication defects: The newly formed SPB is improperly inserted into the nuclear envelope (NE), preventing the cell from forming a bipolar mitotic spindle [8].
  • Here, we characterized the genetic interactions between EAP1 and NDC1, a gene whose function is required for duplication of the spindle pole body (SPB) [12], the centrosome-equivalent organelle in yeast that functions as the centrosome [5].
  • We show that Ndc1p is a shared component of NPCs and SPBs and propose a shared function in the assembly of these organelles into the NE [7].
  • Limited proteolysis of yeast Ndc1p in cellular membranes confirms the orientation of its C-terminus [9].

Physical interactions of NDC1

  • The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p [4].

Other interactions of NDC1

  • Finally, we have found that a deletion of POM152, which encodes an abundant but nonessential nucleoporin, suppresses the SPB duplication defect associated with a mutation in the NDC1 gene [7].
  • Additionally, distinct spots of Ndc1p localization colocalize with a known SPB component, Spc42p [7].
  • Indirect immunofluorescence microscopy shows that Ndc1p displays punctate, nuclear peripheral localization that colocalizes with a known NPC component, Nup49p [7].
  • Order-of-function experiments reveal that the NDC1 function is required in G1 after alpha-factor arrest but before the arrest caused by cdc34 [10].
  • Cells overexpressing NDC1 arrest with monopolar spindles and exhibit increase-in-ploidy phenotypes [6].

Analytical, diagnostic and therapeutic context of NDC1

  • Immunoelectron microscopy shows that Ndc1p localizes to the regions of NPCs and SPBs that interact with the NE [7].
  • Electron microscopy studies reveal that the absence of Ndc1p and Pom152p results in aberrant pores that have enlarged diameters and lack proteinaceous material, leading to an increased diffusion between the cytoplasm and the nucleus [3].


  1. A gene required for the separation of chromosomes on the spindle apparatus in yeast. Thomas, J.H., Botstein, D. Cell (1986) [Pubmed]
  2. The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells. Mansfeld, J., Güttinger, S., Hawryluk-Gara, L.A., Panté, N., Mall, M., Galy, V., Haselmann, U., Mühlhäusser, P., Wozniak, R.W., Mattaj, I.W., Kutay, U., Antonin, W. Mol. Cell (2006) [Pubmed]
  3. The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function. Madrid, A.S., Mancuso, J., Cande, W.Z., Weis, K. J. Cell Biol. (2006) [Pubmed]
  4. The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. Araki, Y., Lau, C.K., Maekawa, H., Jaspersen, S.L., Giddings, T.H., Schiebel, E., Winey, M. Mol. Biol. Cell (2006) [Pubmed]
  5. Yeast Eap1p, an eIF4E-associated protein, has a separate function involving genetic stability. Chial, H.J., Stemm-Wolf, A.J., McBratney, S., Winey, M. Curr. Biol. (2000) [Pubmed]
  6. Altered dosage of the Saccharomyces cerevisiae spindle pole body duplication gene, NDC1, leads to aneuploidy and polyploidy. Chial, H.J., Giddings, T.H., Siewert, E.A., Hoyt, M.A., Winey, M. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  7. Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies. Chial, H.J., Rout, M.P., Giddings, T.H., Winey, M. J. Cell Biol. (1998) [Pubmed]
  8. Mutant membrane protein of the budding yeast spindle pole body is targeted to the endoplasmic reticulum degradation pathway. McBratney, S., Winey, M. Genetics (2002) [Pubmed]
  9. Topology of yeast Ndc1p: predictions for the human NDC1/NET3 homologue. Lau, C.K., Delmar, V.A., Forbes, D.J. The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology. (2006) [Pubmed]
  10. NDC1: a nuclear periphery component required for yeast spindle pole body duplication. Winey, M., Hoyt, M.A., Chan, C., Goetsch, L., Botstein, D., Byers, B. J. Cell Biol. (1993) [Pubmed]
WikiGenes - Universities