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Gene Review

PUB1  -  Pub1p

Saccharomyces cerevisiae S288c

Synonyms: ARS consensus-binding protein ACBP-60, N2842, Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1, Poly uridylate-binding protein, Poly(U)-binding protein, ...
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Disease relevance of PUB1

  • We examined the effects of deletions of genes encoding deadenylase components Ccr4p and Pan2p and putative RNA-binding proteins Pub1p and Puf4p on the genome-wide pattern of mRNA stability after inhibition of transcription by chemicals and/or heat stress [1].

High impact information on PUB1

  • The RNA binding protein Pub1 modulates the stability of transcripts containing upstream open reading frames [2].
  • We have examined global mRNA turnover in isogenic PUB1 and pub1delta strains through gene expression analysis and demonstrate that 573 genes exhibit a significant reduction in half-life in a pub1delta strain [3].
  • The carbon source-regulated effect on MFA2 3'-UTR-controlled translation involves the role of conserved ARE binding proteins, the ELAV/TIA-1-like Pub1p, which can interact with the cap/eIF4G complex, and the translation/mRNA stability factor poly(A) binding protein (Pab1p) [4].
  • These results suggest that the p38 MAPK/Hog1p pathway regulates 3'-UTR-mediated translation by modulating recruitment of Pab1p and Pub1p, which can interact with the translation machinery [4].
  • Among the Pub1p-associated mRNAs, 53 transcripts encoding proteins involved in ribosomal biogenesis and cellular metabolism are selectively destabilized in the pub1delta strain [3].

Biological context of PUB1

  • Even though the PUB1 protein is a major cellular polyadenylated RNA-binding protein, it is nonessential for cell growth [5].

Anatomical context of PUB1


Associations of PUB1 with chemical compounds


Other interactions of PUB1

  • Pan2p and Puf4p also contributed to the degradation rate of these mRNAs following transcriptional shutoff, while Pub1p preferentially stabilized transcripts encoding ribosomal proteins [1].

Analytical, diagnostic and therapeutic context of PUB1

  • UV cross-linking demonstrated that PUB1 is bound to poly(A)+ RNA (mRNA or pre-mRNA) in living cells, and it was detected primarily in the cytoplasm by indirect immunofluorescence [6].
  • This protein, PUB1 [for poly(U)-binding protein 1], was purified by affinity chromatography on immobilized poly(rU), and specific monoclonal antibodies to it were produced [6].
  • Indirect cellular immunofluorescence combined with digital image processing allowed a detailed comparison of the intracellular distributions of PUB1 and PAB1 [5].
  • We also examine the binding specificity of Pub1p using affinity purification followed by microarray analysis to comprehensively distinguish between direct and indirect targets and find that Pub1p significantly binds to 368 cellular transcripts [3].


  1. Genome-wide analysis of mRNA stability using transcription inhibitors and microarrays reveals posttranscriptional control of ribosome biogenesis factors. Grigull, J., Mnaimneh, S., Pootoolal, J., Robinson, M.D., Hughes, T.R. Mol. Cell. Biol. (2004) [Pubmed]
  2. The RNA binding protein Pub1 modulates the stability of transcripts containing upstream open reading frames. Ruiz-Echevarría, M.J., Peltz, S.W. Cell (2000) [Pubmed]
  3. Global analysis of Pub1p targets reveals a coordinate control of gene expression through modulation of binding and stability. Duttagupta, R., Tian, B., Wilusz, C.J., Khounh, D.T., Soteropoulos, P., Ouyang, M., Dougherty, J.P., Peltz, S.W. Mol. Cell. Biol. (2005) [Pubmed]
  4. p38 mitogen-activated protein kinase/Hog1p regulates translation of the AU-rich-element-bearing MFA2 transcript. Vasudevan, S., Garneau, N., Tu Khounh, D., Peltz, S.W. Mol. Cell. Biol. (2005) [Pubmed]
  5. PUB1 is a major nuclear and cytoplasmic polyadenylated RNA-binding protein in Saccharomyces cerevisiae. Anderson, J.T., Paddy, M.R., Swanson, M.S. Mol. Cell. Biol. (1993) [Pubmed]
  6. PUB1: a major yeast poly(A)+ RNA-binding protein. Matunis, M.J., Matunis, E.L., Dreyfuss, G. Mol. Cell. Biol. (1993) [Pubmed]
  7. The pub1 E3 ubiquitin ligase negatively regulates leucine uptake in response to NH(4)(+) in fission yeast. Karagiannis, J., Saleki, R., Young, P.G. Curr. Genet. (1999) [Pubmed]
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