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Gene Review

MCM2  -  MCM DNA helicase complex subunit MCM2

Saccharomyces cerevisiae S288c

Synonyms: DNA replication licensing factor MCM2, Minichromosome maintenance protein 2, YBL023C, YBL0438
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High impact information on MCM2

  • A family of related yeast replication proteins, MCM2, 3 and 5 (also called, after cell-division cycle, CDC46), resemble licensing factor, entering the nucleus only during mitosis [1].
  • Cell proliferation and DNA replication defects in a Drosophila MCM2 mutant [2].
  • Once inside the nucleus, a fraction of the MCM2 and MCM3 proteins becomes tightly associated with DNA [3].
  • We show further that the nuclear and subnuclear localizations of the MCM2 and MCM3 proteins are temporally regulated with respect to the cell cycle [3].
  • MCM2 encodes a protein of 890 amino acids containing a putative zinc-finger domain that is essential for Mcm2 function [4].

Biological context of MCM2


Anatomical context of MCM2


Associations of MCM2 with chemical compounds


Physical interactions of MCM2

  • Here we show that both Cdc45p and replication protein A (RPA) bind to Mcm2p at the G(1)-S transition in an S-CDK-dependent manner [10].

Other interactions of MCM2

  • Furthermore, overproduction of Mcm3 accentuates the deleterious effect of the mcm2-1 mutation, whereas overproduction of Mcm2 partially complements the mcm3-1 mutation [4].
  • Class 1 includes genes that encode components of the pre-RC and pre-IC and are represented by SLM3, 4 and 5 which are allelic to MCM7, MCM2 and CDC45, respectively [11].
  • Significantly, when pretreated with alkaline phosphatase, Mcm2p became completely inactive as a substrate, suggesting that it must be phosphorylated by other protein kinase(s) to be a substrate for the Cdc7p/Dbf4p complex [12].
  • Cdc45p is required for recruiting DNA polymerase alpha onto chromatin, and it associates with Mcm2p, RPA, and DNA polymerase epsilon only during S phase [10].
  • We have assayed the kinase activity of endogenous levels of Cdc7p kinase by using a likely physiological target, Mcm2p, as a substrate [13].

Analytical, diagnostic and therapeutic context of MCM2


  1. MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells. Madine, M.A., Khoo, C.Y., Mills, A.D., Laskey, R.A. Nature (1995) [Pubmed]
  2. Cell proliferation and DNA replication defects in a Drosophila MCM2 mutant. Treisman, J.E., Follette, P.J., O'Farrell, P.H., Rubin, G.M. Genes Dev. (1995) [Pubmed]
  3. Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast. Yan, H., Merchant, A.M., Tye, B.K. Genes Dev. (1993) [Pubmed]
  4. Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function. Yan, H., Gibson, S., Tye, B.K. Genes Dev. (1991) [Pubmed]
  5. Phosphorylation of MCM4 by Cdc7 Kinase Facilitates Its Interaction with Cdc45 on the Chromatin. Masai, H., Taniyama, C., Ogino, K., Matsui, E., Kakusho, N., Matsumoto, S., Kim, J.M., Ishii, A., Tanaka, T., Kobayashi, T., Tamai, K., Ohtani, K., Arai, K. J. Biol. Chem. (2006) [Pubmed]
  6. Fission yeast cdc21+ belongs to a family of proteins involved in an early step of chromosome replication. Coxon, A., Maundrell, K., Kearsey, S.E. Nucleic Acids Res. (1992) [Pubmed]
  7. Identification of Cdc6 protein domains involved in interaction with Mcm2 protein and Cdc4 protein in budding yeast cells. Jang, S.W., Elsasser, S., Campbell, J.L., Kim, J. Biochem. J. (2001) [Pubmed]
  8. Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replication. Eide, T., Taskén, K.A., Carlson, C., Williams, G., Jahnsen, T., Taskén, K., Collas, P. J. Biol. Chem. (2003) [Pubmed]
  9. Overlapping roles of the spindle assembly and DNA damage checkpoints in the cell-cycle response to altered chromosomes in Saccharomyces cerevisiae. Garber, P.M., Rine, J. Genetics (2002) [Pubmed]
  10. Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled by S-phase cyclin-dependent kinases and Cdc7p-Dbf4p kinase. Zou, L., Stillman, B. Mol. Cell. Biol. (2000) [Pubmed]
  11. Budding yeast mcm10/dna43 mutant requires a novel repair pathway for viability. Araki, Y., Kawasaki, Y., Sasanuma, H., Tye, B.K., Sugino, A. Genes Cells (2003) [Pubmed]
  12. Characterization of the yeast Cdc7p/Dbf4p complex purified from insect cells. Its protein kinase activity is regulated by Rad53p. Kihara, M., Nakai, W., Asano, S., Suzuki, A., Kitada, K., Kawasaki, Y., Johnston, L.H., Sugino, A. J. Biol. Chem. (2000) [Pubmed]
  13. Cell cycle control of Cdc7p kinase activity through regulation of Dbf4p stability. Oshiro, G., Owens, J.C., Shellman, Y., Sclafani, R.A., Li, J.J. Mol. Cell. Biol. (1999) [Pubmed]
  14. The role and regulation of the preRC component Cdc6 in the initiation of premeiotic DNA replication. Ofir, Y., Sagee, S., Guttmann-Raviv, N., Pnueli, L., Kassir, Y. Mol. Biol. Cell (2004) [Pubmed]
  15. The roles of the MCM, ORC, and Cdc6 proteins in determining the replication competence of chromatin in quiescent cells. Madine, M.A., Swietlik, M., Pelizon, C., Romanowski, P., Mills, A.D., Laskey, R.A. J. Struct. Biol. (2000) [Pubmed]
  16. Identification and complete cDNA sequence of the missing Drosophila MCMs: DmMCM3, DmMCM6 and DmMCM7. Feger, G. Gene (1999) [Pubmed]
  17. Genome-wide mapping of ORC and Mcm2p binding sites on tiling arrays and identification of essential ARS consensus sequences in S. cerevisiae. Xu, W., Aparicio, J.G., Aparicio, O.M., Tavar??, S. BMC Genomics (2006) [Pubmed]
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