The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

SPT2  -  Spt2p

Saccharomyces cerevisiae S288c

Synonyms: EXA1, Negative regulator of Ty transcription, Protein SPT2, SIN1, SPM2, ...
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Psychiatry related information on SPT2

  • Administration of NOS inhibitors and NO scavenger Hb increased the pain threshold capacity significantly, whereas NO donors SIN-1, SNP and NO precursor L-arginine were found to be hyperalgesic [1].
 

High impact information on SPT2

  • Deletion of the SIN1 gene, which codes for a DNA-binding protein that negatively regulates HO transcription, restores INO1 transcription and reduces the cold sensitivity of such strains [2].
  • Recruitment of mRNA cleavage/polyadenylation machinery by the yeast chromatin protein Sin1p/Spt2p [3].
  • This conclusion is based on the following findings: Sin1p/Spt2 frequently binds specifically downstream of many ORFs but almost always upstream of the first polyadenylation site [3].
  • Furthermore, using recombinant molecules in vitro, we show that the N terminal of SIN1 is sufficient to bind a portion of CDC23 consisting solely of tetratrico peptide repeats [4].
  • In an effort to understand the basis for these phenotypes, we employed the yeast two-hybrid system to identify proteins that interact with SIN1 in vivo [4].
 

Biological context of SPT2

  • We show here that several alleles carrying C-terminal deletions as well as point mutations in the C-terminal domain of the SPT2 protein exhibit a dominant suppressor phenotype [5].
  • We have cloned SIN1 and found that it is identical to the previously identified gene SPT2, mutations in which allow transcription from certain mutated regulatory regions [6].
  • Because sin1 mutants exhibit increased loss of chromosome III, SIN1 may also play a role in fidelity of chromosome segregation [6].
  • To assess the significance of this structural similarity and identify domains of SPT2 functionally important in the regulation of his4-912 delta, we have studied recessive and dominant spt2 mutations created by in vitro mutagenesis [5].
  • When the DNA sequence to which the protein complex binds is placed in a CYC1 promoter lacking a UAS (upstream activating sequence), it can serve as a weak UAS in a SIN1 dependent way [7].
 

Anatomical context of SPT2

 

Associations of SPT2 with chemical compounds

 

Physical interactions of SPT2

  • SIN1 interacts with a protein that binds the URS1 region of the yeast HO gene [7].
  • Furthermore, we show, using recombinant molecules in vitro, that a short 27 amino acid sequence near the N-terminal of SIN1 is sufficient to bind SAP1 [13].
 

Regulatory relationships of SPT2

  • The ability of spt2 mutations to suppress the transcriptional interference caused by the delta promoter insertion his-4-912 delta correlates with an increase in wild-type HIS4 mRNA levels [5].
  • Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner [14].
 

Other interactions of SPT2

  • Addition of random DNA segments to a test promoter alters regulation by SIN1 in a manner similar to addition of a segment from the HO upstream region [6].
  • The Saccharomyces cerevisiae SPT2 gene was identified by genetic screens for mutations which are suppressors of Ty and delta insertional mutations at the HIS4 locus [5].
  • The predicted SIN1/SPT2 protein has a distinctive amino acid composition (45% charged residues, 25% basic and 20% acidic) and has similarity to the mammalian HMG1 protein, a nonhistone component of chromatin [6].
  • We therefore attempted to walk to RAD4 from the neighboring SPT2 gene and obtained an integrating derivative of a plasmid isolated by Roeder et al [15].
  • Association of yeast SAP1, a novel member of the 'AAA' ATPase family of proteins, with the chromatin protein SIN1 [13].
 

Analytical, diagnostic and therapeutic context of SPT2

  • In addition, genome-wide and gene-specific chromatin immunoprecipitation analyses suggest that Spt2 is primarily associated with coding regions in a transcription-dependent fashion [16].
  • Sequence alignment of Sin1p/Spt2p homologues from 11 different yeast species showed conservation of several domains [9].
  • Reliability and sensitivity of the SPT1- and SPT2-based biosensors were good, but varied with the wastewater [17].

References

  1. Effect of centrally administered nitric oxide modulators in Brewer's yeast-induced nociception in rats. Sarma, J., Tandan, S.K., Hajare, S.W., Kumar, D., Raviprakash, V. Indian J. Exp. Biol. (2000) [Pubmed]
  2. A functional interaction between the C-terminal domain of RNA polymerase II and the negative regulator SIN1. Peterson, C.L., Kruger, W., Herskowitz, I. Cell (1991) [Pubmed]
  3. Recruitment of mRNA cleavage/polyadenylation machinery by the yeast chromatin protein Sin1p/Spt2p. Hershkovits, G., Bangio, H., Cohen, R., Katcoff, D.J. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  4. Association of yeast SIN1 with the tetratrico peptide repeats of CDC23. Shpungin, S., Liberzon, A., Bangio, H., Yona, E., Katcoff, D.J. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  5. Mutational and functional analysis of dominant SPT2 (SIN1) suppressor alleles in Saccharomyces cerevisiae. Lefebvre, L., Smith, M. Mol. Cell. Biol. (1993) [Pubmed]
  6. A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1. Kruger, W., Herskowitz, I. Mol. Cell. Biol. (1991) [Pubmed]
  7. SIN1 interacts with a protein that binds the URS1 region of the yeast HO gene. Katcoff, D.J., Yona, E., Hershkovits, G., Friedman, H., Cohen, Y., Dgany, O. Nucleic Acids Res. (1993) [Pubmed]
  8. On-line detection of nitric oxide formation in liquid aqueous phase by electron paramagnetic resonance spectroscopy. Mordvintcev, P., Mülsch, A., Busse, R., Vanin, A. Anal. Biochem. (1991) [Pubmed]
  9. Functional domains of the yeast chromatin protein Sin1p/Spt2p can bind four-way junction and crossing DNA structures. Novoseler, M., Hershkovits, G., Katcoff, D.J. J. Biol. Chem. (2005) [Pubmed]
  10. NO accounts completely for the oxygenated nitrogen species generated by enzymic L-arginine oxygenation. Mülsch, A., Vanin, A., Mordvintcev, P., Hauschildt, S., Busse, R. Biochem. J. (1992) [Pubmed]
  11. Sensitivity of the essential zinc-thiolate moiety of yeast alcohol dehydrogenase to hypochlorite and peroxynitrite. Crow, J.P., Beckman, J.S., McCord, J.M. Biochemistry (1995) [Pubmed]
  12. Virulence of Sporothrix schenckii conidia and yeast cells, and their susceptibility to nitric oxide. Fernandes, K.S., Coelho, A.L., Lopes Bezerra, L.M., Barja-Fidalgo, C. Immunology (2000) [Pubmed]
  13. Association of yeast SAP1, a novel member of the 'AAA' ATPase family of proteins, with the chromatin protein SIN1. Liberzon, A., Shpungin, S., Bangio, H., Yona, E., Katcoff, D.J. FEBS Lett. (1996) [Pubmed]
  14. Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner. Yona, E., Bangio, H., Friedman, Y., Shpungin, S., Katcoff, D.J. FEBS Lett. (1996) [Pubmed]
  15. RAD4 gene of Saccharomyces cerevisiae: molecular cloning and partial characterization of a gene that is inactivated in Escherichia coli. Fleer, R., Nicolet, C.M., Pure, G.A., Friedberg, E.C. Mol. Cell. Biol. (1987) [Pubmed]
  16. Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae. Nourani, A., Robert, F., Winston, F. Mol. Cell. Biol. (2006) [Pubmed]
  17. Characterization of two novel yeast strains used in mediated biosensors for wastewater. Trosok, S.P., Luong, J.H., Juck, D.F., Driscoll, B.T. Can. J. Microbiol. (2002) [Pubmed]
 
WikiGenes - Universities