High impact information on AQP10

• A new member of the aquaporin family (AQP10) has recently been identified in the human small intestine by molecular cloning and in situ hybridization [1].
• Ribonuclease protection assay and northern blotting have demonstrated that AQP10 is expressed in the human duodenum and jejunum [1].
• The objective of this study was to determine the distribution of the AQP10 protein in the human ileum by immunohistochemistry and western blotting using a polyclonal antibody raised against a unique 17-amino acid peptide derived from the human AQP10 sequence [1].
• However, the subcellular distribution of the AQP10 protein and its plasma membrane polarization have not yet been established [1].
• Results revealed that the AQP10 protein is preferentially targeted to the apical membrane domain of absorptive intestinal epithelial cells, whereas AQP3 is located in the basolateral membrane of the cells and AQP1 expression is restricted to the mucosal microvascular endothelia [1].

Biological context of AQP10

• The results indicate that the mouse AQP10 gene is a pseudogene [2].

Anatomical context of AQP10

• AQP10 is the newest member of aquaporins in mammals and expressed selectively in the duodenum and the jejunum in human functioning as aquaglyceroporin [2].
• Xenopus oocytes expressing AQP10 exhibited an increased osmotic water permeability in a mercury-sensitive manner [3].
• Immunohistochemical localization of aquaporin 10 in the apical membranes of the human ileum: a potential pathway for luminal water and small solute absorption [1].

Associations of AQP10 with chemical compounds

• Although AQP10 belongs to the AQP subfamily, which has been characterized by permeability to water and neutral solutes such as urea and glycerol, it was not permeable to urea nor glycerol [3].
• Recently, a new member of aquaporins was reported as AQP10 [Biochem. Biophys. Res. Commun. 287 (2001) 814], which is incompletely spliced to lose the sixth transmembrane domain and has poor water and no glycerol/urea permeabilities [4].

Other interactions of AQP10

• AQP 4 was not detected by Western blot analysis, and we were unable to test for the recently identified AQP10 due to unavailability of antibodies [5].
• In mammalian cells, more than 10 isoforms (AQP0-AQP10) have been identified so far [6].

Analytical, diagnostic and therapeutic context of AQP10

• Genomic Southern blot revealed the mouse AQP10 gene as a single copy gene [2].
• Immunohistochemistry and immunoelectron microscopy demonstrated localization of the AQP10 with an insertion in the capillary endothelium in villi of the small intestine and the isoform without the insertion in the gastro-entero-pancrestic endocrine cells [7].

References