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Gene Review

tufB  -  translation elongation factor EF-Tu 2

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3971, JW3943, kirT, pulT
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Disease relevance of tufB


High impact information on tufB


Biological context of tufB

  • This indicates that autoregulation of chromosomal tufB expression does not occur by modulating transcription initiation at the promoter of the tRNA-tufB operon [8].
  • The mechanism underlying feedback inhibition of tufB expression has been studied in vivo by gene-dosage experiments and by gene and operon fusions involving lacZ [8].
  • The mocimycin resistant phenotype is the result of mutations in each of two genes, tufA and tufB [9].
  • Of particular relevance is, that it enabled us for the first time to obtain a mutant tufB on the chromosome, encoding an EF-TuB resistant to kirromycin [10].
  • Several derivatives of this recombinant plasmid harboring deletions and/or inversions in the tufB regulatory region have been constructed and their properties are described [11].

Anatomical context of tufB

  • We have studied the effect of guanosine-5'-diphosphate-3'-diphosphate (ppGpp) on the transcription of the E. coli tufB and recA operons in a cell-free system containing of purified RNA polymerase holoenzyme [12].

Other interactions of tufB

  • Nucleotide differences between tufA and tufB were found at 13 positions [13].


  1. The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli. An, G., Friesen, J.D. Gene (1980) [Pubmed]
  2. Transcription of the E. coli tufB gene: cotranscription with four tRNA genes and inhibition by guanosine-5'-diphosphate-3'-diphosphate. Miyajima, A., Shibuya, M., Kuchino, Y., Kaziro, Y. Mol. Gen. Genet. (1981) [Pubmed]
  3. Mutant ribosomes can generate dominant kirromycin resistance. Tubulekas, I., Buckingham, R.H., Hughes, D. J. Bacteriol. (1991) [Pubmed]
  4. Both genes for EF-Tu in Salmonella typhimurium are individually dispensable for growth. Hughes, D. J. Mol. Biol. (1990) [Pubmed]
  5. The tRNA-tufB operon transcription termination and processing upstream from tufB. Van Delft, J.H., Schmidt, D.S., Bosch, L. J. Mol. Biol. (1987) [Pubmed]
  6. Determinants of DNA sequence divergence between Escherichia coli and Salmonella typhimurium: codon usage, map position, and concerted evolution. Sharp, P.M. J. Mol. Evol. (1991) [Pubmed]
  7. The nucleotide sequence of the Escherichia coli rts gene. Flamm, J.A., Friesen, J.D., Otsuka, A.J. Gene (1988) [Pubmed]
  8. Control of the tRNA-tufB operon in Escherichia coli. 2. Mechanisms of the feedback inhibition of tufB expression studied in vivo and in vitro. Van Delft, J.H., Talens, A., De Jong, P.J., Schmidt, D.S., Bosch, L. Eur. J. Biochem. (1988) [Pubmed]
  9. The isolation and mapping of EF-Tu mutations in Salmonella typhimurium. Hughes, D. Mol. Gen. Genet. (1986) [Pubmed]
  10. Transfer of plasmid-borne tuf mutations to the chromosome as a genetic tool for studying the functioning of EF-TuA and EF-TuB in the E. coli cell. Vijgenboom, E., Bosch, L. Biochimie (1987) [Pubmed]
  11. In vitro construction of the tufB-lacZ fusion: analysis of the regulatory mechanism of tufB promoter. Takebe, Y., Kaziro, Y. Mol. Gen. Genet. (1982) [Pubmed]
  12. Selective inhibition of transcription of the E. coli tufB operon by guanosine-5'-diphosphate-3'-diphosphate. Mizushima-Sugano, J., Miyajima, A., Kaziro, Y. Mol. Gen. Genet. (1983) [Pubmed]
  13. The nucleotide sequence of the cloned tufA gene of Escherichia coli. Yokota, T., Sugisaki, H., Takanami, M., Kaziro, Y. Gene (1980) [Pubmed]
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