Disease relevance of tufB

• The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli [1].
• The transcription of the tufB gene by purified RNA polymerase holoenzyme was studied using the transducing phage lambda rifd 18 DNA and the hybrid plasmid pTUB1 DNA (Miyajima et al. 1979) as templates [2].
• Mutations in the two genes for EF-Tu in Salmonella typhimurium and Escherichia coli, tufA and tufB, can confer resistance to the antibiotic kirromycin [3].

High impact information on tufB

• Eleven independently isolated insertions are described, six in tufA and five in tufB [4].
• The tRNA-tufB operon transcription termination and processing upstream from tufB [5].
• S1 nuclease fine mapping reveals that the processing site is located in the intergenic region at about 72 to 74 nucleotides upstream from the initiation codon of the tufB cistron [5].
• In particular, the duplicate genes encoding elongation factor Tu, tufA and tufB, from S. typhimurium have been compared to their E. coli homologues [6].
• The transformation experiments indicate that the rts gene is distinct from the flanking birA, tRNA and tufB genes [7].

Biological context of tufB

• This indicates that autoregulation of chromosomal tufB expression does not occur by modulating transcription initiation at the promoter of the tRNA-tufB operon [8].
• The mechanism underlying feedback inhibition of tufB expression has been studied in vivo by gene-dosage experiments and by gene and operon fusions involving lacZ [8].
• The mocimycin resistant phenotype is the result of mutations in each of two genes, tufA and tufB [9].
• Of particular relevance is, that it enabled us for the first time to obtain a mutant tufB on the chromosome, encoding an EF-TuB resistant to kirromycin [10].
• Several derivatives of this recombinant plasmid harboring deletions and/or inversions in the tufB regulatory region have been constructed and their properties are described [11].

Anatomical context of tufB

• We have studied the effect of guanosine-5'-diphosphate-3'-diphosphate (ppGpp) on the transcription of the E. coli tufB and recA operons in a cell-free system containing of purified RNA polymerase holoenzyme [12].

Other interactions of tufB

• Nucleotide differences between tufA and tufB were found at 13 positions [13].

References