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Gene Review

tufA  -  translation elongation factor EF-Tu 1

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3326, JW3301, kirT, pulT
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Disease relevance of tufA


High impact information on tufA

  • Together with targeted mutagenesis of the tufA gene, conditions were found to overcome membrane impermeability, thereby allowing the selection of three mutants harbouring elongation factor (EF)-Tu Arg230-->Cys, Arg333-->Cys or Thr334-->Ala which confer pulvomycin resistance [6].
  • This gene maps in the same context as the tufA gene of Escherichia coli str operon [7].
  • Eleven independently isolated insertions are described, six in tufA and five in tufB [8].
  • Reverse transcription-PCR (RT-PCR) methods were developed for detecting mRNA from rpoH, groEL, and tufA genes. mRNA from all three genes was detected immediately after the cells had been killed by heat or ethanol but gradually disappeared with time when dead cells were held at room temperature [9].
  • An M13-based genetic retrieval system reported by Zeef and Bosch [Mol. Gen. Genet. 238 (1993) 252-260] was used to segregate and identify an extremely growth-defective kirromycin-resistant (KrR) tufA mutation, encoding Gln124-->Lys (Q124K), from a KrR parent strain [10].

Biological context of tufA


Anatomical context of tufA


Other interactions of tufA

  • Nucleotide differences between tufA and tufB were found at 13 positions [1].
  • Measurements of transcription rates from various regions of the str operon in cells carrying the fus gene and the beginning of the tufA gene on a high copy number plasmid confirmed that transcription was initiated within a 600 bp EcoRI fragment in the distal portion of the fus gene [15].

Analytical, diagnostic and therapeutic context of tufA


  1. The nucleotide sequence of the cloned tufA gene of Escherichia coli. Yokota, T., Sugisaki, H., Takanami, M., Kaziro, Y. Gene (1980) [Pubmed]
  2. Duplication of the tuf gene: a new insight into the phylogeny of eubacteria. Sela, S., Yogev, D., Razin, S., Bercovier, H. J. Bacteriol. (1989) [Pubmed]
  3. Sequence of the tufA gene encoding elongation factor EF-Tu from Thermus aquaticus and overproduction of the protein in Escherichia coli. Voss, R.H., Hartmann, R.K., Lippmann, C., Alexander, C., Jahn, O., Erdmann, V.A. Eur. J. Biochem. (1992) [Pubmed]
  4. tuf gene dosage effects on the intracellular concentration of EF-TuB. van der Meide, P.H., Kastelein, R.A., Vijgenboom, E., Bosch, L. Eur. J. Biochem. (1983) [Pubmed]
  5. Determination of the number of tuf genes in Chlamydia trachomatis and Neisseria gonorrhoeae. Goldstein, B.P., Zaffaroni, G., Tiboni, O., Amiri, B., Denaro, M. FEMS Microbiol. Lett. (1989) [Pubmed]
  6. Pulvomycin-resistant mutants of E.coli elongation factor Tu. Zeef, L.A., Bosch, L., Anborgh, P.H., Cetin, R., Parmeggiani, A., Hilgenfeld, R. EMBO J. (1994) [Pubmed]
  7. Structure and expression of elongation factor Tu from Bacillus stearothermophilus. Krásný, L., Mesters, J.R., Tieleman, L.N., Kraal, B., Fucík, V., Hilgenfeld, R., Jonák, J. J. Mol. Biol. (1998) [Pubmed]
  8. Both genes for EF-Tu in Salmonella typhimurium are individually dispensable for growth. Hughes, D. J. Mol. Biol. (1990) [Pubmed]
  9. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Sheridan, G.E., Masters, C.I., Shallcross, J.A., MacKey, B.M. Appl. Environ. Microbiol. (1998) [Pubmed]
  10. A growth-defective kirromycin-resistant EF-Tu Escherichia coli mutant and a spontaneously evolved suppression of the defect. Zeef, L.A., Mesters, J.R., Kraal, B., Bosch, L. Gene (1995) [Pubmed]
  11. Evidence for an internal promoter preceding tufA in the str operon of Escherichia coli. An, G., Lee, J.S., Friesen, J.D. J. Bacteriol. (1982) [Pubmed]
  12. The isolation and mapping of EF-Tu mutations in Salmonella typhimurium. Hughes, D. Mol. Gen. Genet. (1986) [Pubmed]
  13. Darkness and antibiotics increase the steady-state transcripts of the elongation factor gene (tuf) in Chlamydomonas reinhardtii. Silk, G.W., Wu, M. Curr. Genet. (1988) [Pubmed]
  14. Rates of growth, ribosome synthesis and elongation factor synthesis in a tufA defective strain of E. coli. Gausing, K. Mol. Gen. Genet. (1981) [Pubmed]
  15. Mapping of two promoters for elongation factor Tu within the structural gene for elongation factor G. Zengel, J.M., Lindahl, L. Biochim. Biophys. Acta (1990) [Pubmed]
  16. Genetic engineering, isolation and characterization of a truncated Escherichia coli elongation factor Tu comprising domains 2 and 3. Pieper, U., Ehbrecht, H.J., Fliess, A., Schick, B., Jurnak, F., Pingoud, A. Biochim. Biophys. Acta (1990) [Pubmed]
  17. Antibiotic resistance mechanisms of mutant EF-Tu species in Escherichia coli. Kraal, B., Zeef, L.A., Mesters, J.R., Boon, K., Vorstenbosch, E.L., Bosch, L., Anborgh, P.H., Parmeggiani, A., Hilgenfeld, R. Biochem. Cell Biol. (1995) [Pubmed]
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