Disease relevance of appY

• The appY gene was mapped to 13 min on the E. coli genetic map [1].

High impact information on appY

• A second DpiA target, the promoter for a gene (appY) implicated in E. coli's response to anaerobiosis, is repressed by DpiA [2].
• The expression and transcriptional regulation of the Escherichia coli cyx-appA operon and the appY gene have been investigated under different environmental conditions with single-copy transcriptional lacZ fusions [3].
• Effect of growth conditions on expression of the acid phosphatase (cyx-appA) operon and the appY gene, which encodes a transcriptional activator of Escherichia coli [3].
• The transcriptional activator AppY is required for anaerobic and stationary-phase induction of the cyx-appA and hya operons of Escherichia coli, and expression of the appY gene itself is induced by these environmental conditions [4].
• The sequence of the appY gene and its promoter region is unusually AT rich [4].

Chemical compound and disease context of appY

• For the CitA-CitB (DpiB-DpiA) two-component signal transduction system from Escherichia coli, three diverse functions have been reported: induction of the citrate fermentation genes citCDEFXGT, repression of the regulator gene appY, and destabilization of the inheritance of iteron-containing plasmids such as pSC101 [5].

Biological context of appY

• The product of the previously identified appY gene, which when present on a high-copy-number plasmid stimulates synthesis of acid phosphatase, was shown to activate the cyx promoter [6].
• An insertion mutation in the appY gene was constructed in vitro and recombined into the chromosome [6].
• The position of the appY gene on the 4.9-kilobase HindIII fragment of the original clone was located by Tn5 mutagenesis and deletion analysis, and the nucleotide sequence of a 1.9-kilobase region containing the gene was determined [1].
• Isolation, characterization, and nucleotide sequence of appY, a regulatory gene for growth-phase-dependent gene expression in Escherichia coli [1].
• The expression of the appY gene is not affected significantly by the anaerobic energy metabolism, i.e., fermentation versus anaerobic respiration [3].

Other interactions of appY

• Location of the ompT gene relative to that of appY on the physical map of the Escherichia coli chromosome [7].
• The appY and crl genes encode transcriptional regulators related to anaerobic energy metabolism and the formation of curli surface fibers, respectively [8].

Analytical, diagnostic and therapeutic context of appY

• Judged by two-dimensional gel electrophoresis, the appY promoter fragment exhibits the features characteristic of curved DNA [4].
• Gel retardation assays showed that purified H-NS protein bound with high affinity to two different segments of the appY promoter region [4].

References