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Gene Review

nadC  -  quinolinate phosphoribosyltransferase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0108, JW0105
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Disease relevance of nadC

  • Genetic and molecular characterization of the guaC-nadC-aroP region of Escherichia coli K-12 [1].
  • Several types of transducing phages, lambda nadC and lambda lpd, carrying the nadC and lpd genes were isolated from populations of artificially constructed transducing phages containing R.HindIII or R.EcoRI fragments of bacterial DNA, by selecting for their ability to complement the metabolic lesions of the corresponding mutants [2].
  • The Salmonella typhimurium nadC gene and its product, quinolinic acid phosphoribosyltransferase (QAPRTase), were characterized at the molecular and biochemical levels [3].
  • The gene associated with the loss of growth promotion in H41 was shown to exhibit 65% identity at the amino acid level to the nadC gene encoding quinolinate phosphoribosyltransferase (QAPRTase) in Ralstonia solanacearum [4].

High impact information on nadC

  • The procedure, a form of runoff DNA synthesis on molecules released from lysed bacterial cells, mapped precisely the position of cleavage of the plasmid pMV158 leading strand origin in Streptococcus pneumoniae and the site of strand scission, nic, at the transfer origins of F and the F-like plasmid R1 in Escherichia coli [5].
  • Cleavage at the F plasmid nic site within the origin of transfer (oriT) requires the F-encoded proteins TraY and TraI and the host-encoded protein integration host factor in vitro [6].
  • Site-specific recombination between wild-type and mutant pCU1 oriTs also demonstrated that point mutations to the right of nic reduced both initiation and termination of transfer while point mutations to the left of nic reduced termination but had little or no effect on initiation [7].
  • A 28-bp deletion within the AT-rich region 39 bases to the right of nic reduced both initiation and termination, while deletion of a 6-bp inverted repeat sequence at the right-most boundary of the minimal oriT region reduced initiation but not termination [7].
  • Adjacent to cooA are two genes, nadB and nadC, with predicted products similar to proteins in other bacteria that catalyze reactions in the de novo synthesis of NAD.(ABSTRACT TRUNCATED AT 250 WORDS)[8]

Chemical compound and disease context of nadC

  • The guaC (GMP reductase), nadC (quinolinate phosphoribosyltransferase), and aroP (aromatic amino acid permease) genes of Escherichia coli K-12 were located in the 2.5-min region of the chromosome (muT-guaC-nadC-aroP-aceE) by a combination of linkage analysis, deletion mapping, restriction analysis, and plasmid subcloning [1].
  • The mutation ampD2 in one such mutant was caused by an IS1 insertion into the hitherto unknown ampD gene, located between nadC and aroP at minute 2.4 on the E. coli chromosome [9].
  • Introduction of the human cDNA into a QPRTase defective (nadC) E. coli strain brought about an abrupt increase in QPRTase activity and allowed the cells to grow in the absence of nicotinic acid [10].

Biological context of nadC

  • The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing [11].
  • The region that retains the origin of replication, nic, mob, and rep genes of the broad-host-range plasmid RSF1010 was isolated as either an HincII or a PstI-PvuII restriction fragment [12].

Associations of nadC with chemical compounds

  • Evidence is also presented which indicates that the product of the nadC locus in S. typhimurium LT-2 is the enzyme quinolinic acid phosphoribosyltransferase [13].

Other interactions of nadC


Analytical, diagnostic and therapeutic context of nadC


  1. Genetic and molecular characterization of the guaC-nadC-aroP region of Escherichia coli K-12. Roberts, R.E., Lienhard, C.I., Gaines, C.G., Smith, J.M., Guest, J.R. J. Bacteriol. (1988) [Pubmed]
  2. Molecular cloning of the pyruvate dehydrogenase complex genes of Escherichia coli. Guest, J.R., Stephens, P.E. J. Gen. Microbiol. (1980) [Pubmed]
  3. The Salmonella typhimurium nadC gene: sequence determination by use of Mud-P22 and purification of quinolinate phosphoribosyltransferase. Hughes, K.T., Dessen, A., Gray, J.P., Grubmeyer, C. J. Bacteriol. (1993) [Pubmed]
  4. Involvement of quinolinate phosphoribosyl transferase in promotion of potato growth by a Burkholderia strain. Wang, K., Conn, K., Lazarovits, G. Appl. Environ. Microbiol. (2006) [Pubmed]
  5. Specific cleavage of chromosomal and plasmid DNA strands in gram-positive and gram-negative bacteria can be detected with nucleotide resolution. Zechner, E.L., Prüger, H., Grohmann, E., Espinosa, M., Högenauer, G. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  6. Mobilization of chimeric oriT plasmids by F and R100-1: role of relaxosome formation in defining plasmid specificity. Fekete, R.A., Frost, L.S. J. Bacteriol. (2000) [Pubmed]
  7. Localization of the nic site of IncN conjugative plasmid pCU1 through formation of a hybrid oriT. Paterson, E.S., Iyer, V.N. J. Bacteriol. (1997) [Pubmed]
  8. Carbon monoxide-induced activation of gene expression in Rhodospirillum rubrum requires the product of cooA, a member of the cyclic AMP receptor protein family of transcriptional regulators. Shelver, D., Kerby, R.L., He, Y., Roberts, G.P. J. Bacteriol. (1995) [Pubmed]
  9. Inactivation of the ampD gene causes semiconstitutive overproduction of the inducible Citrobacter freundii beta-lactamase. Lindberg, F., Lindquist, S., Normark, S. J. Bacteriol. (1987) [Pubmed]
  10. Characterization and functional expression of the cDNA encoding human brain quinolinate phosphoribosyltransferase. Fukuoka, S.I., Nyaruhucha, C.M., Shibata, K. Biochim. Biophys. Acta (1998) [Pubmed]
  11. The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Chambers, S.P., Prior, S.E., Barstow, D.A., Minton, N.P. Gene (1988) [Pubmed]
  12. A broad-host-range vector system for cloning and translational lacZ fusion analysis. Tai, T.N., Havelka, W.A., Kaplan, S. Plasmid (1988) [Pubmed]
  13. Mapping and characterization of the nad genes in Salmonella typhimurium LT-2. Foster, J.W., Moat, A.G. J. Bacteriol. (1978) [Pubmed]
  14. Conjugative transfer of promiscuous IncP plasmids: interaction of plasmid-encoded products with the transfer origin. Fürste, J.P., Pansegrau, W., Ziegelin, G., Kröger, M., Lanka, E. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
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