Disease relevance of Pestivirus

• The predicted zinc binding motif consists of four cysteine residues, conserved among the Hepacivirus and Pestivirus genera, fitting the formula of CX17CXCX20C [1].
• Recently we tentatively identified, by sequence comparison, central domains of the NS3 proteins of flaviviruses and the respective portion of the pestivirus polyprotein as RNA helicases (A.E.G. et al., submitted) [2].
• Mutations abrogating the RNase activity in glycoprotein E(rns) of the pestivirus classical swine fever virus lead to virus attenuation [3].
• Proteomics computational analyses suggest that hepatitis C virus (HCV) envelope glycoprotein E1 and pestivirus envelope glycoprotein E2 are truncated class II fusion proteins [4].
• The pestivirus E(rns) glycoprotein interacts with E2 in both infected cells and mature virions [5].

High impact information on Pestivirus

• Possible role of pestiviruses in microcephaly [6].
• The pestivirus envelope glycoprotein E(rns) has RNase activity and therefore was suspected to enter cells to cleave RNA [7].
• Inspection of the context of AUGs revealed that the polyprotein initiation AUG of pestivirus has a weak context for efficient translation initiation [8].
• N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases [2].
• The core protein of pestiviruses is released from the polyprotein by viral and cellular proteinases [9].

Chemical compound and disease context of Pestivirus

• Thus, the acridone scaffold, when appropriately functionalized, can yield compounds with selective activity against pestiviruses and related viruses such as the HCV [10].
• Extensive analyses of amino acid sequences for the viral structural proteins and nonstructural protein p54 regions from five pestiviruses led to the identification of four conserved domains (designated as C1, C2, C3, C4) and three highly variable domains (designated as V1, V2, V3) within this region [11].
• Immunohistochemical detection of pestivirus was poor in cases of ovine abortion, ovine hairy shaker syndrome, and caprine abortion [12].
• Two months later the flock was reconstituted and after a further three weeks, the ewes were bred to pestivirus negative rams after synchronisation of oestrus using progesterone sponges [13].
• Action of celgosivir (6 O-butanoyl castanospermine) against the pestivirus BVDV: implications for the treatment of hepatitis C [14].

Biological context of Pestivirus

• Preceding the polyprotein start codon, multiple cryptic AUG codons and several small open reading frames are characteristic for all the five pestiviruses [8].
• The pestivirus bovine viral diarrhea virus (BVDV) p80 protein (referred to here as the NS3 protein) contains amino acid sequence motifs predictive of three enzymatic activities: serine proteinase, nucleoside triphosphatase, and RNA helicase [15].
• Ribosomal S27a coding sequences upstream of ubiquitin coding sequences in the genome of a pestivirus [16].

Gene context of Pestivirus

• Insertion of cellular NEDD8 coding sequences in a pestivirus [17].
• Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication [18].
• Processing of a pestivirus protein by a cellular protease specific for light chain 3 of microtubule-associated proteins [19].
• Comparison of the deduced amino acid sequence of X818 with the ones of other pestiviruses allowed the prediction of polyprotein cleavage sites which were conserved with regard to the structural proteins [20].
• Cell-derived sequences in the N-terminal region of the polyprotein of a cytopathogenic pestivirus [21].

References