Disease relevance of Bacteriophage Typing

• The results showed good discriminatory ability of antibiotic-resistance pattern phenotyping and phage-typing when the phages used were adapted to epidemic-associated MRSA strains [1].
• Methicillin-resistant strains of Staphylococcus aureus isolated at the Royal Prince Alfred Hospital since 1965 were differentiated by phage-typing and by their lysogenic status [2].

High impact information on Bacteriophage Typing

• Molecular analysis showed two distinct MRSA phenotypes with similar phage-typing patterns [3].
• The nearly 700 methicillin-resistant cultures that have isolated since 1965 exhibited, with rare exceptions, conventional group-II patterns of lysis in phage-typing and similar antibiotypes [4].
• There were associations with AFLP results and those from phage-typing and cadmium and arsenic resistance typing, although each method showed some independence [5].
• Isolates were characterised at the sub-species level by Penner serology, Preston biotyping, Preston phage-typing, as well as E3CJC2 restriction fragment length polymorphism (RFLP), 16S ribotyping, flagellin (flaA) RFLP and single-stranded conformational polymorphism (SSCP) analyses [6].
• Serotyping yielded five, phage-typing eight, OMP profile analysis nine and PFGE seven distinct types of P. aeruginosa [7].

Biological context of Bacteriophage Typing

• Two distinct strains of S. aureus could be defined, differing in phage-typing patterns, the exfoliative toxin produced, plasmid profile, cadmium resistance and bacteriocin production [8].
• This report also presents data from 172 cases of MRSA, including carriage rate, infection site, antibiogram, phage-typing, treatment and/or clearance outcome and preliminary evaluation of pathogenicity [9].

Associations of Bacteriophage Typing with chemical compounds

• Five other patients were colonized by a second strain, gentamicin-resistant and non-typable by phage-typing, probably introduced into the hospital 12 months previously by a patient from Nairobi, Kenya. A 12th patient was colonized by both strains simultaneously [10].
• The cluster followed discontinuation of hexachlorophene for scrub of the extremity prior to cannulation; phage-typing suggested the three cases were caused by the patients' own strains of Staphylococcus [11].

Gene context of Bacteriophage Typing

• The isolates from this HCW and patient contacts were shown to be indistinguishable by antibiogram and repetitive extragenic palindromic polymerase chain reaction (REP/PCR); none were typeable by lytic phage-typing [12].

Analytical, diagnostic and therapeutic context of Bacteriophage Typing

• A representative selection of 65 isolates of MRSA over a period spanning five years (1994-1998) was subjected to antibiogram comparison, phage-typing and pulsed field gel electrophoresis (PFGE) using SmaI [13].

References