Disease relevance of INDOPHENOL

• An isolate of Pseudomonas aeruginosa lacking characteristic indophenol oxidase was recovered from a catheterized urine specimen [1].
• Arterial ammonia with Blood Ammonia Checker II and with indophenol reaction to assess presence of hepatic encephalopathy [2].
• A case of overwhelming neonatal meningitis and sepsis is discussed, and the use of the simple indophenol oxidase test on laboratory isolates of gram-negative rods is urged to distinguish this organism and its close relatives from the Enterobacteriaceae [3].

High impact information on INDOPHENOL

• We show that an indophenol-based colorimetric method for acetaminophen (Clin Toxicol 15: 67-73, 1979) has less than maximal sensitivity because of (a) incomplete hydrolysis, related to suboptimal acid concentration; (b) suboptimal hydrolysis time; and (c) possible deterioration of the ammonium hydroxide reagent after 2.5 months [4].
• A sensitive, optimized assay for serum AMP deaminase (EC 3.5.4.6) is presented, which is based on a colorimetric indophenol determination of ammonia liberated during the enzyme reaction [5].
• These isolates were gram-negative, oxidative rods which were motile by means of a single polar flagellum; gave positive catalase, indophenol oxidase, urease, and amylase reactions; and grew slowly at 30 degrees C. Fourteen isolates conformed to the designated type strains Pseudomonas mesophilica ATCC 29983 and Protaminobacter ruber ATCC 8457 [6].
• Two isolates of Pseudomonas aeruginosa lacking characteristic indophenol oxidase were recovered from a sputum specimen [7].
• 2,6-Dichloro-phenol indophenol prevents switch-over of electrons between the cyanide-sensitive and -insensitive pathway of the mitochondrial electron transport chain in the presence of inhibitors [8].

Biological context of INDOPHENOL

• The genetic diversity of each locus varied between 0.095 for indophenol oxidase and 0.881 for the fast variety of malic enzyme [9].

Anatomical context of INDOPHENOL

• Tris-washed, 2,6-dichlorophenol indophenol-treated grana are low in both Mn content and oxygen-evolving activity [10].
• Determination of ammonia in cerebrospinal fluid using the indophenol direct method [11].
• Isolated tonoplast and plasma membranes reduced ferricyanide, indophenol and various iron complexes with NADH or NADPH as electron donors [12].

Associations of INDOPHENOL with other chemical compounds

• Spectrophotometric determination of oxyphenbutazone in drug formulations using the indophenol reaction [13].
• The third assay involved urease-catalyzed hydrolysis of allantoate-derived urea, followed by reaction of the released ammonia to form indophenol [14].
• Sensitivity improvement of ammonia determination based on flow-injection indophenol spectrophotometry with manganese(II) ion as a catalyst and analysis of exhaust gas of thermal power plant [15].

Gene context of INDOPHENOL

• Urea produced by arginase was hydrolyzed to ammonia by urease, the ammonia was converted to indophenol, and the absorbance was measured at 570 nm [16].
• In reverse micelles, some inhibitors of AChE (propidium, and d-tubocurarine) have much less effect upon indophenol-acetate hydrolysis than they do in a dilute solution environment [17].

Analytical, diagnostic and therapeutic context of INDOPHENOL

• Analysis is carried out in end-point samples by Winkler's titration and indophenol-blue for oxygen and ammonia-N, respectively [18].

References