Disease relevance of hydroxyazanium

• Moreover, 15-60 mmol/L ammonium hydroxide produced severe macroscopic gastric lesions in the rats subjected to ischemia [1].
• An open, randomized, human-model study was conducted to compare the effects of topical antibiotics, a wound protectant, and antiseptics on the rate of wound healing and bacterial growth using a modification of a method employing ammonium hydroxide-induced intradermal blisters inoculated with Staphylococcus aureus [2].
• Cellular polysaccharide fractions of various representative members of genera of the family Spirochaetaceae were obtained by the ammonium hydroxide extraction method [3].

High impact information on hydroxyazanium

• The exposure of the stomach to 15-60 mmol/L ammonium hydroxide induced both a reduction in transmucosal potential difference and microscopic damage to the gastric mucosa in normotensive rats [1].
• Whereas ammonium hydroxide or hypotonic lysis resulted in relatively high levels of matrix-associated TF, virtually no TF was found on the matrix after mild enzymatic detachment of stimulated ECs [4].
• Extracellular matrix derived from TNF-treated endothelium, prepared after removing the cells by hypotonic lysis or ammonium hydroxide (0.1 N), also had similarly enhanced TF activity [5].
• PI10-containing complexes are dissociated with conditions known to separate classical protease-serpin complexes (i.e., 1.5 m ammonium hydroxide in the presence of SDS) [6].
• After removal of the cells in culture adherent to glass with 25 mM ammonium hydroxide, indirect immunofluorescence demonstrated an exuberant residual extracellular residue enriched with fibronectin, laminin, collagen type IV, and procollagen type III [7].

Biological context of hydroxyazanium

• The method combines ammonium hydroxide hydrolysis and matrix assisted laser desorption ionization (MALDI)-mass spectrometry analysis and has been successfully proven with five different Lipid A species [8].
• The acid metabolite of antrafenine was measured by gas-liquid chromatography with electron-capture detection using a 1 m column packed with 3% OV-225 on Gas-Chrom Q (100-120 mesh) at 240 degrees C and on-column methylation with trimethylphenyl ammonium hydroxide [9].
• We tested the hypothesis that AE conditions [60 mM tetraethyl ammonium hydroxide (TEA), 20 mM EDTA, pH 12.6, for 16 h at room temperature] dissociate DDC but not DPC using chromium(III)-treated plasmid DNA and the RAGE assay [10].
• During stimulation of flow by brief inhalation of ammonium hydroxide fumes, the concentration of each compound was lower [11].
• Kinetics of phthalate reactions with ammonium hydroxide in aqueous matrix [12].

Anatomical context of hydroxyazanium

• SDS or ammonium hydroxide was used to extract the epithelium, which was then removed by physical shearing [13].
• Preservation and visualization of these filaments and their arrangements improved considerably when cytoskeletons were imaged with phosphotungstic acid buffered with ammonium hydroxide (PTA(NH4] [14].
• METHODS: Bruch's membrane explants were prepared from 16 human cadaver eyes (7 eyes age <50 years; 9 eyes >50 years) by removing native RPE cells with ammonium hydroxide to expose the RPE cell basal lamina (BL) [15].
• Concentration of ammonium hydroxide is critical in chromatographic solvents for amniotic fluid phospholipids [16].
• The results indicate that hydrogen peroxide and windshield washer fluid or ammonium hydroxide disinfectant may be suitable laboratory disinfectants against C. parvum oocysts [17].

Associations of hydroxyazanium with other chemical compounds

• The testing of different solvents showed that addition of buffers such as ammonium acetate, ammonium hydroxide, or acetic acid may suppress ionization in APPI [18].
• LCACoAs were separated with high resolution using a C18 reversed-phase column at high pH (10.5) with an ammonium hydroxide and acetonitrile gradient [19].
• Aqueous ammonium hydroxide solutions of the analyte are shown to be preferable to sodium hydroxide solutions for negative-ion ESI due to the production of multiply sodiated protein species from the latter system [20].
• We examined the stability of uric acid in dilute aqueous ammonium hydroxide solution by mass spectrometry [21].
• Here, we investigated how backdiffusion of luminal HCl or NH(4)OH and pentagastrin-stimulated acid secretion interact in the communication between rat stomach and brain stem [22].

Gene context of hydroxyazanium

• The total activity of LPL, measured in ammonium hydroxide extracts of acetone ether tissue powders, was in the low normal range in the hypothyroid patients (0.68 +/- 0.42), but not significantly different from normal (1.10 +/- 0.58) and did not increase significantly (92 +/- 105%), with treatment [23].
• METHODS: Confluent first-to third-passage human RPE were harvested from tissue culture and plated onto RPE-derived extracellular matrix or human Bruch's membrane exoplants denuded of cells by treatment with 0.02 N ammonium hydroxide [24].
• We conclude that vagal afferents transmit physiological stimuli (gastrin) and pathological events (backdiffusion of luminal HCl or NH(4)OH) from the stomach to the brain stem [22].
• For concentrated ammonium hydroxide, an on-line electrochemical neutralizer (SP10 AutoNeutralization module) was used to neutralize the base prior to the IC analysis [25].
• 1. Ammonium hydroxide treatment of [des-Asn1]-EGF (beta-EGF) did not produce conversion to EGF-I [26].

Analytical, diagnostic and therapeutic context of hydroxyazanium

• The dansylated products, which are eluted from the resin with ammonium hydroxide, can be identified rapidly by thin layer chromatography [27].
• The adducts were characterized by their UV absorption spectra, electrospray ionization mass spectrometry (ESIMS), and facile conversion from 7-methoxy- to 7-amino-substituted mitosene type adducts upon 10% NH(4)OH treatment, which were identical with known adducts of MC [28].
• Separation of PLs was carried out on an HPLC silica gel column, with a mobile phase consisting of chloroform, methanol and ammonium hydroxide, and detection was performed with a light-scattering evaporative detector [29].
• Effect of ammonium hydroxide concentration on the recoveries of amino acids during preparation for gas-liquid chromatography [30].
• The total amount of water-soluble acids in the extract was validated by potentiometric titration with tetrabutyl ammonium hydroxide [31].

References