Disease relevance of Fluorescin

• With binocular indirect ophthalmoscopy, fluorescin angiography and ultrasonography 68 choroidal metastases in 52 eyes of 39 patients were diagnosed [1].

High impact information on Fluorescin

• After exposure to a polyclonal rabbit antibody raised to purified brain TFA and subsequent exposure to a fluorescin-labeled murine anti-rabbit IgG, the cell fluorescent activity was analyzed by flow cytometry [2].
• The means of the corneal peak autofluorescence values in the NIDDM, IDDM and penetrating keratoplasty patient groups were significantly higher than that in the healthy control group (mean values in ng equivalent fluorescin ml-1: 18.0 +/- 4.2 S.D., 20.6 +/- 5.1 S.D., 17.9 +/- 5.5 S.D. and 13.7 +/- 3.7 S.D., respectively; P less than 0.01) [3].
• Distribution of microtubules and other cytoskeletal filaments in growing skeletal muscle cells (myotubes) was studied in vitro by fluorescence microscopy using fluorescin-labeled antibodies and phalloidin, a specific antiactin drug [4].
• Counting T-lymphocyte subpopulations (includes T3, T4, T8, T-IL2R, and NK cells) by using fluorescin isothiocyanate or phycoerythrin was performed preoperatively and on 1,2,3,5,7, and 10 d postoperatively [5].
• The increase in hemadsorption is accompanied by an alteration in distribution of receptors within the cell population as measured by flow microfluorometry using fluorescin conjugated concanavalin A, and by a decrease in the total number of receptors as measured by binding of radiolabelled concanavalin A [6].

Biological context of Fluorescin

• A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL-60 by assessing oxidation of 2',7'-dichloro fluorescin diacetate (DCFH-DA) [7].
• In the case of fluorescin (FL), which has a higher partition coefficient than CF, the percutaneous absorption of FL was more enhanced by the addition of AZ than in the case of CF [8].

Anatomical context of Fluorescin

• Fluorescin angiography showed multiple discrete lesions in the pigment epithelium not coincident with the ophthalmoscopically visible changes [9].
• Moderate to strong immunofluorescence of megakaryocytes was noticed in bone marrow smears stained with rabbit anticanine globulin conjugated with fluorescin isothiocyanate [10].
• In this study, assessment by a flow cytometric method using dichlorhydroxy fluorescin diacetate (DCFADH) in vitro revealed that human peripheral blood inhibits the production of active oxygen species by human peripheral neutrophils [11].

References