Disease relevance of Reuterin

• Recent findings indicate that the newly discovered Lactobacillus reuteri reuterin system may be used for this purpose [1].
• This substance, termed reuterin, inhibits the growth of gram-positive and gram-negative bacteria as well as yeasts, fungi, and protozoa [2].
• Natural antimicrobial agent (reuterin) produced by lactobacillus reuteri for sanitization of biological tissues inoculated with pseudomonas aeruginosa [3].
• Strains of L. monocytogenes were more resistant to reuterin than E. coli strains [4].
• The effect of Reuterin on Listeria monocytogenes and Escherichia coli O157:H7 in milk and cottage cheese [4].

High impact information on Reuterin

• Trimethylsilyl derivatives of reuterin were analyzed by gas chromatography-mass spectrometry, and a molecule was identified which had a molecular weight corresponding to a disilylated dimeric structure [2].
• Nuclear magnetic resonance studies of purified reuterin carried out with deuterium oxide confirmed the presence of two three-carbon compounds, beta-hydroxypropionaldehyde and the corresponding hydrated acetal, and a six-carbon cyclic dimer of the aldehyde [2].
• The latter was obtained from a sequence which commenced from the silyl ether 24 of 3-hydroxypropionaldehyde and entailed cyclization of (Z)-zeta-hydroxy-alpha,beta-unsaturated ester 82 [5].
• Additionally, the rate of tissue fixation by reuterin was investigated using glutaraldehyde as a control [6].
• Furthermore, for a large proportion of cells, reuterin completely prevented the cell division within the time of observation [7].

Chemical compound and disease context of Reuterin

• To overcome the aforementioned deficiency with the glutaraldehyde-fixed bioprostheses, a fixation technique using a natural compound (reuterin) produced by Lactobacillus reuteri to crosslink biological tissues was developed by our group [8].
• Glycerol metabolism in Lactobacillus collinoides: production of 3-hydroxypropionaldehyde, a precursor of acrolein [9].
• The impact of glucose on glycerol metabolism, especially on 3-hydroxypropionaldehyde (3-HPA) accumulation by resting cells of Lactobacillus reuteri has been investigated [10].

Biological context of Reuterin

• The lyophilized supernatants (approximately 4000 activity units (AU) of reuterin per ml) were diluted in distilled water for decontamination and preservation trials [11].
• A deterministic mathematical model consisting of four coupled differential equations describing the concentrations of biomass, glucose, glycerol and reuterin with time was developed [12].

Anatomical context of Reuterin

• This strain showed a high survival after exposition to conditions simulating those existing in the gastrointestinal tract as well as a notable ability to adhere to intestinal cells, which suggests that its reuterin-producing ability may be used for the host benefit [13].

Gene context of Reuterin

• The inhibitory effect of reuterin on Escherichia coli K12 was investigated at different conditions of temperature (10-30 degrees C), pH (4.5-6.5), and NaCl (0.5-3% (w/v)) [14].

Analytical, diagnostic and therapeutic context of Reuterin

• In this report, we describe procedures for (i) producing reuterin in sufficient amounts to isolate from a fermentation mixture and (ii) isolating this substance by high-performance liquid chromatography [15].
• The degree of tissue fixation by reuterin is significantly affected by its fixation conditions [6].

References