Disease relevance of ANGPTL7

• Human ANGPTL7 mRNA was expressed in neural tissues, keratoconus cornea, trabecular meshwork, melanotic melanoma and uterus endometrial cancer, while mouse Angptl7 mRNA was expressed in four-cell embryo, synovial fibroblasts, thymus, uterus and testis [1].
• Expression of CDT6 led to a reduction in tumor growth and aberrant blood vessel formation by inducing massive fibrosis [2].
• MATERIALS AND METHODS: In this report we describe the generation of a stably CDT6-expressing clone of the murine melanoma cell line B16-F10 [3].

High impact information on ANGPTL7

• These observations strongly suggest a role as morphogen for CDT6 in inducing a corneal phenotype in vivo [2].
• One abundant TM transcript was from the gene for the angiopoietin-like factor CTD6, which is located at on the long arm of chromosome 1, area 36.2-36.1 in the region of the glaucoma locus GLC3B, whereas other transcripts were from genes close to known glaucoma loci [4].
• METHODS: Rapid amplification of cDNA ends (5'-RACE) was used to isolate the CDT6 promoter from an adaptor-ligated genomic DNA fragment library and to identify the transcription initiation site of the CDT6 gene [5].
• The human CDT6 promoter contains four interferon (IFN)-stimulated response elements (ISREs) [5].
• The sequence similarity, binding sites for putative transcription factors, and transcriptional activity of human and mouse CDT6 promoters were compared [5].

Biological context of ANGPTL7

• Human ANGPTL7, characterized as potent target gene of WNT/ beta-catenin signaling pathway, is a pharmacogenomics target in the fields of oncology and regenerative medicine [1].
• Because four TCF/LEF-binding sites were identified within the human ANGPTL7 promoter, comparative genomics analyses on ANGPTL7 orthologs were further performed [1].
• ANGPTL7 gene at human chromosome 1p36.22 was located within intron 28 of FRAP1 gene encoding mTOR protein [1].
• Subsequently, the full cDNA was sequenced, and the identified open reading frame was named cornea-derived transcript 6 (CDT6) [6].
• The major transcription initiation sites of the human and mouse CDT6 genes were located at 224 and 168 bp, respectively, upstream of the translation initiation site [5].

Anatomical context of ANGPTL7

• In addition no effect of the CDT6 gene product was found on proliferation of endothelial cells in vitro [3].

Associations of ANGPTL7 with chemical compounds

• Similar to the angiopoietins, CDT6 contains a hydrophobic NH2-terminal sequence, a coiled-coil domain, and a COOH-terminal fibrinogenlike domain [6].

Analytical, diagnostic and therapeutic context of ANGPTL7

• To test this hypothesis and to assay the effect of the protein on a growing tissue with high vascular density, CDT6 was expressed in a mouse xenograft model [2].

References