High impact information on RUVBL2

• In this study, we isolated a new TIP49a-related gene, termed TIP49b, from human and yeast cells [1].
• Interestingly, TIP48 formed oligomers in the presence of adenine nucleotides, whilst TIP49 did not [2].
• Three-dimensional reconstruction at 20 A resolution revealed that the TIP48/TIP49 complex consisted of two stacked hexameric rings with C6 symmetry [2].
• Relocalization of human chromatin remodeling cofactor TIP48 in mitosis [3].
• This redeployment of TIP48 during anaphase and cytokinesis was independent of microtubule assembly [3].

Associations of RUVBL2 with chemical compounds

• On the other hand, HuH7 cells with an overexpression of RUVBL2 grew better in soft agar (P < 0.03), had increased resistance to C2 ceramide-induced apoptosis (P < 0.001), and gave rise to significantly larger tumors when injected into immunodeficient Rag2/gammac mice (P = 0.016) [4].

Biological context of RUVBL2

• Analysis of the nucleotide sequences revealed that ECP-51 and ECP-54 are homologous (44.2% amino acid identity) and contain ATP-binding sites [5].
• Accumulation of TIP48 in the midzone and the midbody was observed in late telophase and cytokinesis [3].
• As the cells entered mitosis, TIP48 was excluded from the condensing chromosomes but showed association with the mitotic apparatus [3].
• Our studies demonstrate that in interphase cells TIP48 was found mainly in the nucleus and exhibited a distinct localization in the nuclear periphery [3].
• However, we observed distinct changes in the subcellular localization of TIP48 during M phase using immunofluorescence microscopy [3].

Anatomical context of RUVBL2

• TIP49b/RUVBL2, like RUVBL1, was expressed ubiquitously in all human tissues examined and more strongly in testis [6].
• We isolated two proteins, ECP-51 and ECP-54, from human erythrocyte cytosol by affinity chromatography using a peptide of the integral membrane protein stomatin as bait [5].
• Like TIP49a, TIP49b was abundantly expressed in the testis and thymus [1].
• We show that TIP48 abundance in HeLa cells did not change during the cell cycle, nor did its distribution in various biochemical fractions [3].
• During anaphase, some TIP48 was detected in the centrosome colocalizing with tubulin but the strongest staining appeared in the mitotic equator associated with the midzone central spindle [3].

Physical interactions of RUVBL2

• The structure of the TIP48/TIP49 complex was examined by negative stain electron microscopy [2].

Other interactions of RUVBL2

• The very close co-location of the mouse homologues of the human TIP49b/RUVBL2 and LHB genes was also conserved on mouse chromosome 7 [6].

Analytical, diagnostic and therapeutic context of RUVBL2

• 3. Genomic sequence analysis revealed that the TIP49b/RUVBL2 gene is very close (55 nucleotides in length) to the LHB gene, in the opposite orientation [6].

References