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MeSH Review:

Mitotic Spindle Apparatus

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Disease relevance of Mitotic Spindle Apparatus

Localization of NGF and TrkA at mitotic apparatus in human glioma cell line U251 [1].
Mitotic apparatus and nucleoli compartmentalization of 50,000-dalton type II regulatory subunit of cAMP-dependent protein kinase in estrogen receptor negative MDA-MB-231 human breast cancer cells [2].

High impact information on Mitotic Spindle Apparatus

CPEB interacts with microtubules and is involved in the localization of cyclin B1 mRNA to the mitotic apparatus [3].
In interphase cells NuMA protein is restricted to the nucleus and is a constituent of isolated nuclear matrices, but in mitotic cells it is observed by indirect immunofluorescence microscopy to be concentrated at the polar regions of the mitotic apparatus [4].
The protein composition of mitotic apparatus was not dramatically altered by treatment with 0.5% Triton X-100, even though vesicles were destroyed and yolk was extracted [5].
Indanocine interacts with tubulin at the colchicine-binding site, potently inhibits tubulin polymerization in vitro, and disrupts the mitotic apparatus in dividing cells [6].
A human homologue of the Drosophila warts tumor suppressor, h-warts/LATS1, is an evolutionarily conserved serine/threonine kinase and a dynamic component of the mitotic apparatus [7].

Biological context of Mitotic Spindle Apparatus

Antibodies to cyclic AMP, cyclic GMP, and the type I regulatory and catalytic subunits of the cyclic AMP-dependent protein kinase did not label the mitotic apparatus [8].
During mitosis, CaM kinase II was found to be a dynamic component of the mitotic apparatus, particularly present at microtubule-organizing centers [9].
This paper reports that there are major differences between mammalian cell lines in the propensity to progress into subsequent cell cycles when mitosis is inhibited with agents that disrupt the assembly of the mitotic spindle apparatus (Colcemid, nocodazole, and taxol) [10].
When 2-mercaptoethanol is removed, the chromosomes reorient to the poles of a tetrapolar (sometimes tripolar) mitotic apparatus [11].
The striking presence of PKC zeta in the central portion of the mitotic apparatus suggests a functional role for this kinase isoform in cell division [12].

Anatomical context of Mitotic Spindle Apparatus

Previous studies suggest that kinesin heavy chain (KHC) is associated with ER-derived membranes that accumulate in the mitotic apparatus in cells of early sea urchin embryos (Wright, B. D., J. H. Henson, K. P. Wedaman, P. J. Willy, J. N. Morand, and J. M. Scholey. 1991. J. Cell Biol. 113:817-833) [13].
Associating with the mitotic apparatus, EB1 may play a physiologic role connecting APC to cellular division, coordinating the control of normal growth and differentiation processes in the colonic epithelium [14].
The localization of CaM kinase II in the cell nucleus and the mitotic apparatus suggests that the enzyme may play a role in the cell cycle progression of mammalian cells [9].
To determine the function of mammalian ELPs, we generated antibodies against a 70-kDa human ELP and showed that ELP70 coassembled with MTs in HeLa cell extracts and colocalized with MTs in the mitotic apparatus [15].
Protein kinase C zeta is associated with the mitotic apparatus in primary cell cultures of the shark rectal gland [12].

Associations of Mitotic Spindle Apparatus with chemical compounds

To investigate the function of calmodulin (CaM) in the mitotic apparatus, the effect of microinjected CaM and chemically modified CaMs on nocodazole-induced depolymerization of spindle microtubules was examined [16].
Identification of nonmitochondrial creatine kinase enzymatic activity in isolated sea urchin mitotic apparatus [17].
Mitotic apparatus isolated with vesicles intact converted ADP to ATP if phosphocreatine was present [17].
We studied sea-urchin zygote mitotic apparatus (MA) isolated in hexylene glycol, transferred immediately to a glycerol-dimethylsulphoxide medium, and stored for 2 weeks at room temperature [18].
Mass isolation of mitotic apparatus using a glycerol/Mg2+/Triton X-100 medium [19].

Gene context of Mitotic Spindle Apparatus

Transforming acidic coiled-coil-containing protein 4 interacts with centrosomal AKAP350 and the mitotic spindle apparatus [20].
We have shown here that the kinesin-2 complex is localized with components of the mitotic apparatus such as spindle microtubules and centrosomes [21].
Cytocentrin is a Ral-binding protein involved in the assembly and function of the mitotic apparatus [22].
In dividing clonal striatal cells, HAP-1 localized to the mitotic spindle apparatus, especially at spindle poles and on vesicles and microtubules of the spindle body [23].
We propose that a pathway involving Ndc10 might monitor defects in the mitotic apparatus independently of the Mad and Bub proteins [24].

Analytical, diagnostic and therapeutic context of Mitotic Spindle Apparatus

In this report, we physically manipulated the proximity of the mitotic apparatus (MA) to the cell cortex in combination with microinjection of effectors of the spindle checkpoint and CDK1 activity to determine how the initiation of cytokinesis is coupled to the onset of anaphase and mitotic exit [25].
A total of 11 differentially expressed protein spots corresponding to 10 independent proteins were detected, and peptide fingerprinting combined with mass spectrometry of these proteins revealed them to include NuMA (nuclear protein that associates with the mitotic apparatus), heat shock proteins, and redox regulators [26].
The morphologies of interphase MTs and the mitotic spindle apparatus were analysed by immunofluorescence staining of alpha-tubulin [27].

References

Localization of NGF and TrkA at mitotic apparatus in human glioma cell line U251. Zhang, Z., Yang, Y., Gong, A., Wang, C., Liang, Y., Chen, Y. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
Mitotic apparatus and nucleoli compartmentalization of 50,000-dalton type II regulatory subunit of cAMP-dependent protein kinase in estrogen receptor negative MDA-MB-231 human breast cancer cells. Kapoor, C.L., Cho-Chung, Y.S. Cell Biol. Int. Rep. (1983) [Pubmed]
CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: implications for local translational control of cell division. Groisman, I., Huang, Y.S., Mendez, R., Cao, Q., Theurkauf, W., Richter, J.D. Cell (2000) [Pubmed]
Human-specific nuclear protein that associates with the polar region of the mitotic apparatus: distribution in a human/hamster hybrid cell. Lydersen, B.K., Pettijohn, D.E. Cell (1980) [Pubmed]
Isolation of mitotic apparatus containing vesicles with calcium sequestration activity. Silver, R.B., Cole, R.D., Cande, W.Z. Cell (1980) [Pubmed]
Indanocine, a microtubule-binding indanone and a selective inducer of apoptosis in multidrug-resistant cancer cells. Leoni, L.M., Hamel, E., Genini, D., Shih, H., Carrera, C.J., Cottam, H.B., Carson, D.A. J. Natl. Cancer Inst. (2000) [Pubmed]
Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor. Hirota, T., Morisaki, T., Nishiyama, Y., Marumoto, T., Tada, K., Hara, T., Masuko, N., Inagaki, M., Hatakeyama, K., Saya, H. J. Cell Biol. (2000) [Pubmed]
Immunofluorescent localization of cyclic nucleotide-dependent protein kinases on the mitotic apparatus of cultured cells. Browne, C.L., Lockwood, A.H., Su, J.L., Beavo, J.A., Steiner, A.L. J. Cell Biol. (1980) [Pubmed]
Ca2+/calmodulin-dependent protein kinase II: localization in the interphase nucleus and the mitotic apparatus of mammalian cells. Ohta, Y., Ohba, T., Miyamoto, E. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
Cell line-specific differences in the control of cell cycle progression in the absence of mitosis. Kung, A.L., Sherwood, S.W., Schimke, R.T. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
Centrosome detection in sea urchin eggs with a monoclonal antibody against Drosophila intermediate filament proteins: characterization of stages of the division cycle of centrosomes. Schatten, H., Walter, M., Mazia, D., Biessmann, H., Paweletz, N., Coffe, G., Schatten, G. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
Protein kinase C zeta is associated with the mitotic apparatus in primary cell cultures of the shark rectal gland. Lehrich, R.W., Forrest, J.N. J. Biol. Chem. (1994) [Pubmed]
Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection. Wright, B.D., Terasaki, M., Scholey, J.M. J. Cell Biol. (1993) [Pubmed]
The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules. Berrueta, L., Kraeft, S.K., Tirnauer, J.S., Schuyler, S.C., Chen, L.B., Hill, D.E., Pellman, D., Bierer, B.E. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
The human EMAP-like protein-70 (ELP70) is a microtubule destabilizer that localizes to the mitotic apparatus. Eichenmuller, B., Everley, P., Palange, J., Lepley, D., Suprenant, K.A. J. Biol. Chem. (2002) [Pubmed]
Calmodulin stabilization of kinetochore microtubule structure to the effect of nocodazole. Sweet, S.C., Rogers, C.M., Welsh, M.J. J. Cell Biol. (1988) [Pubmed]
Identification of nonmitochondrial creatine kinase enzymatic activity in isolated sea urchin mitotic apparatus. Silver, R.B., Saft, M.S., Taylor, A.R., Cole, R.D. J. Biol. Chem. (1983) [Pubmed]
Spindle birefringence of isolated mitotic apparatus: further evidence for two birefringent spindle components. Forer, A., Kalnins, V.I., Zimmerman, A.M. J. Cell. Sci. (1976) [Pubmed]
Mass isolation of mitotic apparatus using a glycerol/Mg2+/Triton X-100 medium. Sakai, H., Shimoda, S., Hiramoto, Y. Exp. Cell Res. (1977) [Pubmed]
Transforming acidic coiled-coil-containing protein 4 interacts with centrosomal AKAP350 and the mitotic spindle apparatus. Steadman, B.T., Schmidt, P.H., Shanks, R.A., Lapierre, L.A., Goldenring, J.R. J. Biol. Chem. (2002) [Pubmed]
Role of the kinesin-2 family protein, KIF3, during mitosis. Haraguchi, K., Hayashi, T., Jimbo, T., Yamamoto, T., Akiyama, T. J. Biol. Chem. (2006) [Pubmed]
Cytocentrin is a Ral-binding protein involved in the assembly and function of the mitotic apparatus. Quaroni, A., Paul, E.C. J. Cell. Sci. (1999) [Pubmed]
Analysis of Huntingtin-associated protein 1 in mouse brain and immortalized striatal neurons. Martin, E.J., Kim, M., Velier, J., Sapp, E., Lee, H.S., Laforet, G., Won, L., Chase, K., Bhide, P.G., Heller, A., Aronin, N., Difiglia, M. J. Comp. Neurol. (1999) [Pubmed]
Role of the kinetochore protein Ndc10 in mitotic checkpoint activation in Saccharomyces cerevisiae. Fraschini, R., Beretta, A., Lucchini, G., Piatti, S. Mol. Genet. Genomics (2001) [Pubmed]
Transitions regulating the timing of cytokinesis in embryonic cells. Shuster, C.B., Burgess, D.R. Curr. Biol. (2002) [Pubmed]
Proteomic analysis of hematopoietic stem cell-like fractions in leukemic disorders. Ota, J., Yamashita, Y., Okawa, K., Kisanuki, H., Fujiwara, S., Ishikawa, M., Lim Choi, Y., Ueno, S., Ohki, R., Koinuma, K., Wada, T., Compton, D., Kadoya, T., Mano, H. Oncogene (2003) [Pubmed]
Photodynamic effects of the cationic porphyrin, mesotetra(4N-methylpyridyl)porphine, on microtubules of HeLa cells. Juarranz, A., Villanueva, A., Díaz, V., Cañete, M. J. Photochem. Photobiol. B, Biol. (1995) [Pubmed]

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