Disease relevance of Abpa

• The transmembrane transcriptional activators ToxR and TcpP modulate expression of Vibrio cholerae virulence factors by exerting control over toxT, which encodes the cytoplasmic transcriptional activator of the ctx, tcp, and acf virulence genes [1].
• This gene was also used to construct transfectants of the CH12 lymphoma clone CH12.LX, which express the Abp gene product in association with the endogenous A alpha k chain [2].

High impact information on Abpa

• The requirements of the virulence regulators, ToxR, TcpP, and ToxT, for expression of tcpA and ctxA were determined and were found to differ significantly during infection versus during growth in vitro [3].
• This is consistent with the elevated evolutionary rates observed for the Abpa gene, and the Abpb and Abpg genes whose products (ABPbeta and ABPgamma) form heterodimers with ABPalpha [4].
• Allelic variation within the mouse androgen-binding protein (ABP) alpha subunit gene (Abpa) has been suggested to promote assortative mating and thus prezygotic isolation [4].
• We have investigated the mouse sequence that contains the three Abpa/b/g genes, and orthologous regions in rat, human, and chimpanzee genomes [4].
• The ratio of polymorphism to divergence was significantly lower at Abpa than at Glra2 and Amg, despite the fact that all three genes experience similar rates of recombination [5].

Biological context of Abpa

• A study of recombinant inbred strains demonstrates that Abpa is located on chromosome 7 near Glucose phosphate isomerase-1 (Gpi-1) [6].
• Here we report a comparison of the DNA coding sequences and putative amino acid sequences they determine for the three common alleles of the Alpha subunit gene (Abpa), alleles that appear to be diagnostic for the three subspecies of Mus musculus [7].
• Reduced nucleotide variability at an androgen-binding protein locus (Abpa) in house mice: evidence for positive natural selection [5].
• We investigated patterns of DNA sequence variation at Abpa within and between species of mice to test several predictions of a model of neutral molecular evolution [5].
• Intraspecific variation among 10 Mus musculus domesticus alleles was compared with divergence between M. m. domesticus and M. caroli for Abpa and two X-linked genes, Glra2 and Amg [5].

Anatomical context of Abpa

• Finally, we also used reverse transcription-PCR of mRNAs isolated from submandibular salivary glands to determine the Abpa allele in the striped wood mouse and compare it to published information on the homologue in Mus musculus domesticus [8].
• We used a radiolabeled riboprobe and in situ hybridization to mouse (Mus) Abpa mRNA to localize Abpa synthesis to submandibular gland acinar cells [8].

Associations of Abpa with chemical compounds

• Previous work has shown that the gene for the alpha subunit of androgen-binding protein, Abpa, may be involved in premating isolation between different subspecies of the house mouse, Mus musculus [5].
• The SspS allele alters the electrophoretic mobility of Abp in males at puberty or in females which have received exogenous testosterone [Karn, R.C., Dlouhy, S.R., Springer, K.R., Hjorth, J.P., and Nielsen, J.T. (1982). Biochem Genet. 20:493] [9].

Other interactions of Abpa

• Following the inheritance of a restriction fragment length polymorphism associated with MAG coding sequences allowed the locus to be positioned 0.5 centimorgans from the locus Abpa (androgen binding protein alpha) on proximal chromosome 7 [10].

Analytical, diagnostic and therapeutic context of Abpa

• To test if this difference accounts for the MHC influence on susceptibility to collagen-induced arthritis, H-2p mice were made transgenic with an Abp gene altered to resemble the Abq gene [11].

References